Talk:Azide: Difference between revisions
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:::'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en®ion=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; M = 65.01 gยทmol<sup>-1</sup>. | :::'''Azd: Azide''' (Sodium azide, NaN<sub>3</sub>); [http://www.sigmaaldrich.com/catalog/product/sial/s2002?lang=en®ion=GB Sigma S2002], [http://www.sigmaaldrich.com/MSDS/MSDS/DisplayMSDSPage.do?country=GB&language=en&productNumber=S2002&brand=SIAL&PageToGoToURL=http%3A%2F%2Fwww.sigmaaldrich.com%2Fcatalog%2Fproduct%2Fsial%2Fs2002%3Flang%3Den SDS] 25 g, store commercial stock at RT; M = 65.01 gยทmol<sup>-1</sup>. | ||
:::: 2021-02: The preparation instructions were updated to take the volume of the solute into account. The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations. | :::: 2021-02: The preparation instructions were updated to take the volume of the solute into account (see [[User:Spitzer_Gudrun/Volume_of_the_solute]]). The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations. | ||
::::<span style="color:#8B008B"> '''Caution:''' Very toxic!</span> | ::::<span style="color:#8B008B"> '''Caution:''' Very toxic!</span> | ||
Revision as of 11:08, 17 February 2021
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Application in HRR (updated instructions)
- Azd: Azide (Sodium azide, NaN3); Sigma S2002, SDS 25 g, store commercial stock at RT; M = 65.01 gยทmol-1.
- 2021-02: The preparation instructions were updated to take the volume of the solute into account (see User:Spitzer_Gudrun/Volume_of_the_solute). The concentrations prepared following the former instructions (see Discussion section) are sufficiently high for SUIT protocol titrations.
- Caution: Very toxic!
- Azd: Azide (Sodium azide, NaN3); Sigma S2002, SDS 25 g, store commercial stock at RT; M = 65.01 gยทmol-1.
- Preparation of 4 M storage and stock solution (1 mL, dissolved in H2O) for 2-mL O2k-chamber:
- Weigh 260 mg of sodium azide.
- Dissolve in 900 ยตL H20.
- Divide into 0.5 mL portions.
- Store at -20 ยฐC.
- ยปO2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): โฅ50 ยตL using a 100 ยตL Hamilton syringe.
- Final concentration: โฅ100 mM.
- Preparation of 2 M stock solution (dissolved in H2O) for 0.5-mL O2k-chamber:
- Take 400 ยตL of the 4 M stock solution.
- Add 400 ยตL H2O.
- ยปO2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): โฅ25 ยตL using a 100 ยตL Hamilton syringe.
- Final concentration: โฅ100 mM.