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Difference between revisions of "Template:Current week ecosystem agenda"

From Bioblast
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* '''May-04 Mo'''
 
2020 Week 20
 
* '''May-11 Mo'''
 
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
|'''Do you load the correct instrumental background correction according to the actual range of experimental O<sub>2</sub> concentrations?'''<br>
|'''Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.'''<br>
Perform and apply the instrumental background test:<br>
Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis <br>
- »[[Flux / Slope| O2 background correction in DatLab]]« and »[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]«
- »[[Steady state]]«
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|}


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* '''May-05 Tu'''  
* '''May-12 Tu'''  
::::[[Image:O2k-Publications.jpg|60px|left|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]''' Expression of alternative oxidase prevents reverse electron transfer'''
:{|
::::::: - from the O2k-Network »[[FI Helsinki Jacobs HT]]«  »[[DE Magdeburg Gellerich FN]]« »[[FI Tampere Dufour E]]« »[[DE Magdeburg Debska-Vielhaber G]]« »[[DE Frankfurt Wittig I]]« and »[[UK Brighton Moore AL]]«
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
::::::* Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. - [[Szibor 2019 Biochim Biophys Acta Bioenerg|»Bioblast link«]]
| '''Mitochondria - a driving force in adipocyte differentiation''' <br>
:from the O2k-Network »[[CZ Pilsen Kuncova J]]« <br>
* Kladnická I, Čedíková M, Kripnerová M, Dvořáková J, Kohoutová M, Tůma Z, Müllerová D, Kuncová J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96. [[Kladnicka 2019 Physiol Res|»Bioblast link«]]
|}
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* '''May-06 We'''  
* '''May-13 We'''


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* '''May-07 Th'''
* '''May-14 Th'''
:{|
::::* Abstract submission deadline - »[[EMC2020 Prague CZ]]«
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
| Quantify '''[[Fatty acid oxidation pathway control state|fatty acid oxidation capacity (FAO, F-pathway)''']], avoiding a common artefact of overestimation'''. Malate anaplerotic pathways ([[Malic enzyme|see '''mt-malic enzyme''']]) are active in various tissues and cultures cells, which requires caution: »'''[[Malate anaplerotic pathway control state]]'''«


- '''DatLab 7.4''' provides a step-by-step guide to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«


- Since [[Fatty acid oxidation pathway control state|F-pathway capacity]] can be overestimated due to anaplerotic reactions, a reference protocol was designed to avoid this artefact - »'''[[SUIT-002 |SUIT-002]]'''«
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|60px|MitoPedia]]
|How can I measure '''ATP production''' with the O2k?  <br>
Learn more about how to measure ATP production in [[mitochondrial preparations]] with Magnesium Green <br>
- »'''[[Magnesium Green]]'''«
|}
|}
<br />
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* '''May-15 Fr'''
:{|
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
|''' Restoration of mitochondrial oxygen consumption and decrease of mitochondrial ROS by AOX in the post-ischaemic heart are not sufficient to confer acute or chronic cardioprotection in mouse ''' <br>
: from the O2k-Network » [[ FI Helsinki Jacobs HT]] « , » [[ DE Magdeburg Gellerich FN]] « , » [[FI Tampere Dufour E]] « , » [[DE Magdeburg Debska-Vielhaber G]] « and » [[DE Frankfurt Wittig I]]«<br>
* Szibor Marten, Schreckenberg Rolf, Gizatullina Zemfira, Dufour Eric, Wiesnet Marion, Dhandapani Praveen Kumar, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Nyman Tuula A, Gaertner Ulrich, Hall Andrew R, Pell Victoria, Viscomi Carlo, Krieg Thomas, Murphy Michael P, Braun Thomas, Gellerich Frank Norbert, Schlueter Klaus-Dieter, Jacobs Howard T(2020) Respiratory chain signalling is essential for adaptive remodelling following cardiac ischaemia. J Cell Mol Med [Epub ahead of print].  - [[Szibor 2020 J Cell Mol Med|»Bioblast link«]]


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* '''May-08 Fr'''

Revision as of 08:07, 11 May 2020

2020 Week 20
  • May-11 Mo
O2k-Open SupportTake care Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.

Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis
- »Steady state«


  • May-12 Tu
O2k-Publications in the MiPMap Mitochondria - a driving force in adipocyte differentiation
from the O2k-Network »CZ Pilsen Kuncova J« 
  • Kladnická I, Čedíková M, Kripnerová M, Dvořáková J, Kohoutová M, Tůma Z, Müllerová D, Kuncová J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96. »Bioblast link«

  • May-13 We

  • May-14 Th


MitoPedia
How can I measure ATP production with the O2k?

Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green
- »Magnesium Green«


  • May-15 Fr
O2k-brief repository Restoration of mitochondrial oxygen consumption and decrease of mitochondrial ROS by AOX in the post-ischaemic heart are not sufficient to confer acute or chronic cardioprotection in mouse
from the O2k-Network » FI Helsinki Jacobs HT « , » DE Magdeburg Gellerich FN « , » FI Tampere Dufour E « , » DE Magdeburg Debska-Vielhaber G « and » DE Frankfurt Wittig I«
  • Szibor Marten, Schreckenberg Rolf, Gizatullina Zemfira, Dufour Eric, Wiesnet Marion, Dhandapani Praveen Kumar, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Nyman Tuula A, Gaertner Ulrich, Hall Andrew R, Pell Victoria, Viscomi Carlo, Krieg Thomas, Murphy Michael P, Braun Thomas, Gellerich Frank Norbert, Schlueter Klaus-Dieter, Jacobs Howard T(2020) Respiratory chain signalling is essential for adaptive remodelling following cardiac ischaemia. J Cell Mol Med [Epub ahead of print]. - »Bioblast link«