Difference between revisions of "Template:SUIT text D039"
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SUIT-003 O2 ce D039 has been designed to study the [[coupling control state]] of intact [[microalgal]] cells. The different capacities are tested in the sequence [[OXPHOS]], [[LEAK respiration]] and [[Electron transfer pathway]]. Depending on the species of microalgae studied, the [[oligomycin]] or any other inhibitor of the [[ATP synthase]] or of the [[adenine nucleotide translocase]] might not have an effect and the [[LEAK respiration]] cannot be achieved. Therefore, it is recommend to test the effect of these inhibitors in preliminary experiments for different species. Also, the inhibitory effect on the [[Electron transfer pathway|electron transfer system]] has to be studied to avoid an underestimation of the [[ET | SUIT-003 O2 ce D039 has been designed to study the [[coupling control state]] of intact [[microalgal]] cells. The different capacities are tested in the sequence [[OXPHOS]], [[LEAK respiration]] and [[Electron transfer pathway]]. Depending on the species of microalgae studied, the [[oligomycin]] or any other inhibitor of the [[ATP synthase]] or of the [[adenine nucleotide translocase]] might not have an effect and the [[LEAK respiration]] cannot be achieved. Therefore, it is recommend to test the effect of these inhibitors in preliminary experiments for different species. Also, the inhibitory effect on the [[Electron transfer pathway|electron transfer system]] has to be studied to avoid an underestimation of the [[ET capacity]]. This protocol has been optimised specifically for the microalgae species ''Chlamydomonas reinhardtii'' in which oligomycin does not have an effect. | ||
Latest revision as of 16:10, 8 June 2020
SUIT-003 O2 ce D039 has been designed to study the coupling control state of intact microalgal cells. The different capacities are tested in the sequence OXPHOS, LEAK respiration and Electron transfer pathway. Depending on the species of microalgae studied, the oligomycin or any other inhibitor of the ATP synthase or of the adenine nucleotide translocase might not have an effect and the LEAK respiration cannot be achieved. Therefore, it is recommend to test the effect of these inhibitors in preliminary experiments for different species. Also, the inhibitory effect on the electron transfer system has to be studied to avoid an underestimation of the ET capacity. This protocol has been optimised specifically for the microalgae species Chlamydomonas reinhardtii in which oligomycin does not have an effect.
