Difference between revisions of "Template talk:Next oroboros ecosystem agenda"
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::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] How can I measure '''ATP production''' in '''cells'''? | ::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] How can I measure '''ATP production''' in '''cells'''? | ||
:::::::- Respiration measurements with living cells provides information on parameters | :::::::- Respiration measurements with living cells provides information on parameters such as [[free ROUTINE activity]]. MitoPedia may help to clarify why defining it as ATP production is inappropriate - »'''[[Magnesium_Green#Is_it_possible_to_measure_ATP_production_in_the_protocol_SUIT-003_O2_ce_D009.3F|SUIT protocols to measure ATP production]]'''« | ||
:::::::- Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green - »'''[[Magnesium_Green#SUIT_protocol_for_mitochondrial_ATP_production_measurement_with_MgG|Magnesium Green]]'''« | :::::::- Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green - »'''[[Magnesium_Green#SUIT_protocol_for_mitochondrial_ATP_production_measurement_with_MgG|Magnesium Green]]'''« | ||
Revision as of 12:06, 4 May 2020
Bioblast alert 2020
Prep for circular done here: Talk:Bioblast alert 2020
MitoPedia alert
Next
- Jun-14 Th
- - Respiration measurements with living cells provides information on parameters such as free ROUTINE activity. MitoPedia may help to clarify why defining it as ATP production is inappropriate - »SUIT protocols to measure ATP production«
- - Learn more about how to measure ATP production in mitochondrial preparations with Magnesium Green - »Magnesium Green«
Already added to Agenda - Next and to be added to Mitopedia alert
O2k-Publications alert
Next
Already added to Agenda - Next and to be added to O2k-Publications alert
O2k-Open Support alert
Next
- While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.
- Jun-01 Mo
- Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?»Instrumental background« and »TIP2k Filter Papers «
Already added to Agenda - Next and to be added to O2k-Open support alert
- May-18 Mo
- Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?
- See how to clean and dry the electrical connections of the OroboPOS-Connector.-»Cleaning the electrical connections«
- Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?
- May-25 Mo
- Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?
- Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.-»Switch components approach«
- Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?
Removed for correction
- Do you perform the leaky chamber test after chamber assembly?
- - An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly. - »Leaky chamber test«
- Do you perform the leaky chamber test after chamber assembly?
O2k-Open Support alert circulars
O2k-Open Support alert circular 2020(01): in prep
- New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
- See how you can check that the two blue OroboPOS-Holders are intact. - »O2k-OroboPOS-Holder«
- New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
- New Calibration parameters are transferred to experimental DatLab files
- Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - » Oxygen calibration - DatLab«
- Do you perform O2k-Chamber volume calibration?
- The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - »O2k-Chamber MitoPedia«
- Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
- New Calibration parameters are transferred to experimental DatLab files
- The O2 solubility factor varies according to the medium used and influences the air calibration. - »Oxygen solubility factor«== In preparation ==
For discussion
Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020
- Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions:
- Proven -80 °C solid stability for more than 3 years (3 years 7 months)
- Proven -20 °C solid stability for 1 year
- Proven DMSO stability at room temperature for 1 week
- Proven DMSO stability at -20 °C for 6 months (ongoing)
- Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions:
- As part of the H2020 SME NextGen-O2k project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O2 consumption and production: »PhotoBiology«
- Do you perform a stirrer test during oxygen calibration?
- In DatLab 7.4, instrumental DL-Protocols guide you automatically towards performing the stirrer test in the process of air calibration. It shows immediately, if your OroboPOS has a satisfactory response time. Further details in MitoPedia: »Oxygen calibration - DatLab
- Do you perform a stirrer test during oxygen calibration?
- Is the noise of the ‘O2 slope neg’ acceptable?
- If you are not certain that the noise of the ‘O2 slope neg’ is within a suitable range or you want to confirm that your experimental data will not be affected, see further details: MiPNet06.03 POS-calibration-SOP
- Is the noise of the ‘O2 slope neg’ acceptable?
2021
- 2021-01-06 https://www.jstor.org/stable/1706346?seq=1