GM-pathway control state

high-resolution terminology - matching measurements at high-resolution

GM-pathway control state

Description

GM: Glutamate & Malate.

MitoPathway control state: NADH electron transfer-pathway state

The GM-pathway control state (glutamate-malate pathway control state) is established when glutamate&malate are added to isolated mitochondria, permeabilized cells and other mitochondrial preparations. Glutamate and transaminase are responsible for the metabolism of oxaloacetate, comparable to the metabolism with acetyl-CoA and citrate synthase.

Abbreviation: GM

Reference: Gnaiger 2020 BEC MitoPathways

More details

» NADH electron transfer-pathway state

» Additive effect of convergent electron flow

» Respiratory complexes - more than five

» Convergent electron flow

GM_L

GM pathway in the LEAK state can be evaluated in the following SUIT protocols:

• SUIT-011
• SUIT-014

GM_P

GM pathway in the OXPHOS state can be evaluated in the following SUIT protocols:

• SUIT-007

• DL-Protocol for permeabilized cells (pce):

• SUIT-011
• SUIT-014

GM_E

GM pathway in the ET state can be evaluated in the following SUIT protocols:

• SUIT-007

• DL-Protocol for permeabilized cells (pce):

Discussion

In human skeletal muscle mitochondria, respiration with GM in the presence of ADP (GM_P) is identical or 10 % higher than with PM (PM_P). These results on isolated mitochondria agree with permeabilized fibres, although there are reports on respiratory capacity for PM_P being 16% to 25% higher than for GM_P. In fibroblasts, GM_P supports a higher respiratory flux than PM_P.

MitoPedia concepts: SUIT state