MiPNet22.11 O2k-FluoRespirometer manual

                  

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MiPNet22.11 O2k-FluoRespirometer manual

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O2k-FluoRespirometer manual.

» » Versions

Oroboros (2023-03-14) Mitochondr Physiol Network

Abstract: Gnaiger E, Komlódi T, Schmitt S, Cecatto C, Gradl L, Gradl P (2023) O2k-FluoRespirometer manual. Mitochondr Physiol Network 22.11(11):1-16.

Open the pdf document above.

» Current O2k-series: NextGen-O2k Series XB and O2k Series J

» Current software versions DatLab 8.0: MitoPedia: DatLab

• Further details: » MitoPedia: O2k-Open Support

» Product: DatLab 7, O2k-FluoRespirometer, O2k-Catalogue

• O2k-Network Lab: AT_Innsbruck_Oroboros

Important updates

• Version 09 (2021-06-04) Section 3 Electric and lab space requirements

- Update of connections on back of O2k-Series I

• Version 08 (2020-10-17) Section 5.4. O2k-chamber volume calibration

1. Instructions are added for the 0.5-mL O2k-chamber volume calibration (O2k-sV-Module).
2. Volume calibration of 2-mL O2k-chamber: Fill open chamber with 2.07 mL water (not 2.085 or 2.10 mL). The volume of the stopper capillary is 0.065 mL (not 0.085 mL).

Troubleshooting

-See: Defective fluorescence module

Keywords

»Bioblast links: Chamber volume - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

» Volume

• Units: Liter [L]; 1 L = 1 dm³ = 10^-3 m³; 1 mL = 1 cm³; 1 µL = 1 mm³

» O2k-chamber

» The experimental chamber in the instrumental O2k-Chamber

» O2k-Chamber sV

» Calibration of O2k-chamber volume

• On chamber volume

» O2k-chamber

» Chamber volume

» MiPNet09.01 O2k-ParadigmShift

» Cell count and normalization in HRR

» Open chamber

» Closed chamber

• Components of the experimental O2k-chambers

» Stirrer-Bar\white PVDF\15x6 mm

» Stirrer-Bar sV\white PVDF\11.5x6.2 mm

» Stopper\black PEEK\conical Shaft\central Port

» Stopper sV\black PEEK\conical Shaft\central Port

» O-ring\Viton\12.5x1 mm

» O-ring sV\Viton\9.5x1 mm

» Volume-Calibration Ring

» Volume-Calibration Ring sV

» Stopper-Spacer

» Cover-Slip\black

» O2k-Chamber Holder

» O2k-Chamber Holder sV

» OroboPOS-Holder

» OroboPOS-Holder sV

• Maintencance

» O2k-cleaning and ISS

» Quality control: O2k-chamber test

Labels: MiParea: Instruments;methods 

HRR: O2k-Manual, Oxygraph-2k 

O2k, DatLab, DL7, SeriesH, SeriesI, O2k-FluoRespirometer