SUITbrowser

                  

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SUITbrowser

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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 

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Description

Use the SUITbrowser to find the substrate-uncoupler-inhibitor-titration (SUIT) protocol most suitable for addressing your research questions.

Open the SUITbrowser: http://suitbrowser.oroboros.at/

How to find a DL-Protocol (DLP)

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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 

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Each SUIT protocol receives a score from 0 to 3 in different categories that correspond to a research question (e.g. information on coupling control, information on fatty acid oxidation, among others). This score is set depending on the information which one can get from each SUIT protocol (3: this SUIT protocol is ideal, highly informative for the specific question; 2: information provided; 1: information provided, but is not a SUIT protocol mainly focused for the study of this question; 0: the specific information/question is not provided by the SUIT protocol). It is possible to choose multiple research questions at a time, to find the best protocols that address this combination of problems.

Define your experimental strategy

Mitochondrial preparations or living cells

1. Mitochondrial preparations: imt, thom, pce, pti (including pfi)
2. Living cells: ce = vce + dce

Applications

1. O₂
2. H₂O₂ and O₂
3. Mitochondrial ATP production and O₂
4. Membrane potential and O₂

Research questions

The following research questions can be selected in the SUITbrowser in various combinations to provide links to the best SUIT protocols:

• Coupling control

• OXPHOS respiration

• ROUTINE respiration

• Maximum physiological capacity

• Cell viability test

• Crabtree effect

• AOX activity

• Permeable succinate/malonate

• Carbohydrate

• Fatty acid oxidation

• Glutamine/glutamate

• Succinate pathway

• Glycerophosphate

• Q-junction (NS)

• Q-junction (FNS)

• Q-junction (FNSGp)

• CI threshold

• Complex IV activity

• mt outer membrane integrity

• Unspecific ATPase activity

• Optimum digitonin concentration

• Mitochondrial ATP production

• Mitochondrial membrane potential

• H₂O₂ - membrane potential dependence

• H₂O₂ - [O₂] dependence

• H₂O₂ - RET initiated

• H₂O₂ - NADH-pathway initiated

MitoPedia concepts: SUIT concept, SUIT protocol 

MitoPedia methods: Respirometry, Fluorometry 

MitoPedia O2k and high-resolution respirometry: DatLab