Template talk:Next oroboros ecosystem agenda: Difference between revisions
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|'''The central role of | |'''The central role of the Q-junction in mitochondria'''<br> | ||
As a converging point in the electron transfer system (ETS) electrones flow from (''1'') Complex I, (''2'') Complex II, (''3'') electron transferring flavoprotein complex, (''4'') mitochondrial glycerophosphate dehydrogenase complex and (''5'') dihydro-orotate dehydrogenase and other enzyme complexes into the Q-junction then downstream to Complex III.<br> | |||
- »[[ | - »[[Q-junction]]« <br> | ||
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| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | |||
|'''Coenzyme Q - a mobile redox compound in the electron transfer system '''<br> | |||
Coenzyme Q is a lipid composed of a benzoquinone ring with an isoprenoid side chain, two methoxy groups and one methyl group being present in all tissues and cells. In humans, CoQ<sub>10</sub> occurring in the mitochondrial electron transfer system transfers electrons from dehydrogenases downstream to Complex III. <br> | |||
- »[[Coenzyme Q]]« <br> | |||
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Revision as of 11:04, 19 November 2021
- Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
{| | logos | title in bold <br> - link |}
e.g.,
Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?
- »Instrumental background« and »TIP2k Filter Papers«
- See the scheme:
Bioblast alert 2021
Prep for circular:
MitoPedia alert
Next
The central role of the Q-junction in mitochondria
As a converging point in the electron transfer system (ETS) electrones flow from (1) Complex I, (2) Complex II, (3) electron transferring flavoprotein complex, (4) mitochondrial glycerophosphate dehydrogenase complex and (5) dihydro-orotate dehydrogenase and other enzyme complexes into the Q-junction then downstream to Complex III.
- »Q-junction«
Coenzyme Q - a mobile redox compound in the electron transfer system
Coenzyme Q is a lipid composed of a benzoquinone ring with an isoprenoid side chain, two methoxy groups and one methyl group being present in all tissues and cells. In humans, CoQ10 occurring in the mitochondrial electron transfer system transfers electrons from dehydrogenases downstream to Complex III.
- »Coenzyme Q«
O2k-Brief alert
Next
O2k-Publications alert
Author names of O2k-Publications/ Brief for the Agenda vs Bioblast
- 1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: Template alerts.
- Example: Oroboros Ecosystem Agenda example
- This means that we have to edit manually the author names within the O2k-Publications/ Brief alerts (i.e., Antunes Diana (from Bioblast) to Antunes D (to Agenda entries)) if they are long or copy from the gray box.
- 2.Bioblast: SOP: [1] – examples are available using both Surname and First name with long version (e.g., Antunes Diana). If in Bioblast the authors are with short surname in the links please update it to complete surname, guidelines are below.
- 1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: Template alerts.
Guidelines to update a reference
- When creating an O2k-Publication/Brief alert, it is the responsibility of the author to check if the reference is accordingly to the correct guidelines:
- Correct format for a person called “First Name Surname”:
- In the gray box should be “Surname FN” - From where references to be used as O2k-Publication/Brief alerts are copied from.
- In the links should be “ Surname First N”
- How to edit:
- A) Check if all authors with profiles in Bioblast (blue links) have their profiles with full name. If they have, proceed to next step (B). If not, you need to move it following the steps “How to move a profile” below.
- B) Click in “Edit with form”.
- C) The gray box is edited in “Reference” and the links are changed in “Author(s)”. Change it accordingly and click in “save page” at the end of the page.
- How to edit:
How to move a profile (instructions sent by Marija):
So let’s take for example my page: Beno M [2]
- As you can see there is a redirect from Beno Marija to Beno M. What has to be done is to switch the content of those pages. Here are the steps:
- 1. Go to e.g. “Beno M” on edit
- 2. Click on “Move”
- 3. Change the new title to the full name of the person. In my case it will be Beno M to Beno Marija
- As you can see there is a redirect from Beno Marija to Beno M. What has to be done is to switch the content of those pages. Here are the steps:
- 4. Now it should look like that:
- 5. Click on “Move” and leave all the ticked boxes
- 6.Done
- After that you can continue in step B.
- If you have any doubt you can contact Cristiane, Marija or Mario.
Next
- Nov
Version 01
Mitochondrial H2O2 production was discovered 50 years ago by the group of Britton Chance
Check out how to measure H2O2 production using the Amplex UltraRed assay in combination with high-resolution respirometry.
- »Loschen 1971 FEBS Letters« and »Amplex UltraRed« and »O2k-Fluo LED2-Module« »O2k-Fluo Smart-Module«
Version 02
50-year anniversary of discovering mitochondrial H2O2 production
Check out how to measure H2O2 production using the Amplex UltraRed assay in combination with high-resolution respirometry.
- »Loschen 1971 FEBS Letters« and »Amplex UltraRed« and »O2k-Fluo LED2-Module« »O2k-Fluo Smart-Module«
in preparation
- Dec
Exercise reduces mitochondrial content in adipose tissues but does not affect mitochondrial respiration and content in skeletal muscle of prediabetic humans
- Szczerbinski L, Taylor MA, Puchta U, Konopka P, Paszko A, Citko A, Szczerbinski K, Goscik J, Gorska M, Larsen S, Kretowski A (2021) The response of mitochondrial respiration and quantity in skeletal muscle and adipose tissue to exercise in humans with prediabetes. Cells 10:3013. Szczerbinski 2021 Cells
- from the O2k-Network »DK Copenhagen Larsen S«
O2k-Open Support alert
- While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.
Next
- Dec-07 Tu
What is ROUTINE respiration?
ROUTINE respiration is the respiratory activity of living (non-permeabilized) cells in the physiological coupling state, which is controlled by cellular energy demand, energy turnover and the degree of coupling to phosphorylation.
- »Gnaiger 2020 BEC MitoPathways - Chapter 2.4«
- communicated by »Komlodi Timea« and »Schmitt Sabine«
- Dec-09 Th
How do you measure ROUTINE respiration?
ROUTINE respiration (R) of living cells is measured before permeabilization of the cell membrane in absence of exogenous substrates and ADP in many SUIT protocols. Allow R to stabilize for 15-20 min.
- »ROUTINE respiration« and »Doerrier 2018 Methods Mol Biol«
- communicated by »Komlodi Timea« and »Schmitt Sabine«
This one needs a BioBlast link:
- Month-##
When filling in the volume of uncoupler in the DatLab mark or the excel template, should one enter the total volume added or the volume to get to the highest flux?
The volume added until the mark, equal to the highest flux, should be entered. Anything titrated after this mark should be summed to the other volumes before the next mark because this will be considered to calculate the sample dilution.
- »LINK and O2 flux analysis with DatLab 7.4«
- communicated by »Cardoso Luiza« and »[[ ]]«
Under discussion
Removed for correction
- Nov-30 Mo
Noisy oxygen signal? Check the OroboPOS and OroboPOS-Connector to clean electrical connections.
Contaminating grease, salt crystals and moisture can be removed with a dry paper cloth or a paper cloth moistened with water and then absolute ethanol.
- »Cleaning the electrical connections«
- communicated by »Schmitt Sabine« and »Komlodi Timea«
WGT recommended not to use water for cleaning. Erich introduced water for cleaning to remove salt crystals.
Do you perform the leaky chamber test after chamber assembly?
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- »Leaky chamber test« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«
O2k-Open Support alert circulars
In preparation
Oxia: O2 regime controller for HyperOxia to HypOxia in the O2k
- » Learn more here «
MitoFit Preprints
Data analysis templates and MipNets
For discussion
Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions: - Proven -80 °C solid stability for more than 3 years (3 years 7 months)
- Proven -20 °C solid stability for 1 year
- Proven DMSO stability at room temperature for 1 week
- Proven DMSO stability at -20 °C for 6 months (ongoing)
2021
- 2021-01-06 https://www.jstor.org/stable/1706346?seq=1
Postponed events