Citrate synthase: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=CS | |abbr=CS | ||
|description=Condensation of [[oxaloacetate]] with acetyl-CoA yields citrate as an entry into the [[TCA cycle]] | |description=Condensation of [[oxaloacetate]] with acetyl-CoA yields citrate as an entry into the [[TCA cycle]]. CS is located in the mt-matrix. CS activity is frequently used as a functional marker of the amount of mitochondria (mitochondrial elementary marker, ''mtE'') for normalization of respiratory flux. | ||
|info=[[ | |info=[[MiPNet17.04 CitrateSynthase]] | ||
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== Normalization of respiratory flux for CS as a mitochondrial marker == | |||
Communicated by [[Gnaiger Erich]] last update 2020-04-19 | |||
:::: Respiration of living or permeabilized cells, tissue homogenate or permeabilized tissue, and isolated mitochondria can be normalized for CS activity. Then marker-specific oxygen flux, ''J''<sub>O<sub>2</sub>/CS</sub> [pmolโsยญ<sup>-1</sup>โIUยญ<sup>-1</sup>], is a quantitative measure of respiration that reflects mitochondrial quality, independent of mitochondrial density. | |||
:::: Several publications in peer-reviewed journals report respiration normalized for CS activity in units which do not make sense, ''e.g.'' [pmolโs<sup>-ยญ1</sup>โmLยญ<sup>-1</sup>โIUยญ<sup>-1</sup>], such that those numerical results are pointless. Clarification is obtained by strict definitions of normalization of rates ([[BEC 2020.1]]) and dimensional analysis, summarized in [[MiPNet17.04 CitrateSynthase |MiPNet 17.04]]. | |||
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== References == | |||
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::::* [[O2k-Publications: Marker enzyme]] | |||
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|mitopedia topic | |mitopedia topic=Enzyme | ||
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Latest revision as of 16:06, 19 January 2021
Description
Condensation of oxaloacetate with acetyl-CoA yields citrate as an entry into the TCA cycle. CS is located in the mt-matrix. CS activity is frequently used as a functional marker of the amount of mitochondria (mitochondrial elementary marker, mtE) for normalization of respiratory flux.
Abbreviation: CS
Reference: MiPNet17.04 CitrateSynthase
Normalization of respiratory flux for CS as a mitochondrial marker
Communicated by Gnaiger Erich last update 2020-04-19
- Respiration of living or permeabilized cells, tissue homogenate or permeabilized tissue, and isolated mitochondria can be normalized for CS activity. Then marker-specific oxygen flux, JO2/CS [pmolโsยญ-1โIUยญ-1], is a quantitative measure of respiration that reflects mitochondrial quality, independent of mitochondrial density.
- Several publications in peer-reviewed journals report respiration normalized for CS activity in units which do not make sense, e.g. [pmolโs-ยญ1โmLยญ-1โIUยญ-1], such that those numerical results are pointless. Clarification is obtained by strict definitions of normalization of rates (BEC 2020.1) and dimensional analysis, summarized in MiPNet 17.04.
References
Bioblast link | Reference | Year |
---|---|---|
Gnaiger 2020 BEC MitoPathways | Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | 2020 |
BEC 2020.1 doi10.26124bec2020-0001.v1 | Gnaiger E et al โ MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 | 2020 |
MiPNet17.04 CitrateSynthase | Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. | 2020-04-18 |
- Bioblast links: Normalization - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Rate
- ยป Normalization of rate
- ยป Flow
- ยป Oxygen flow
- ยป Flux
- ยป Oxygen flux
- ยป Flux control ratio
- ยป Coupling-control ratio
- ยป Pathway control ratio
- ยป Flux control efficiency
- Rate
- Quantities for normalization
- ยป Count in contrast to Number
- ยป Mitochondrial marker
- ยป O2k-Protocols: mitochondrial and marker-enzymes
- ยป Citrate synthase activity
- Quantities for normalization
- General
- ยป Extensive quantity
- ยป Specific quantity
- ยป Advancement
- ยป Motive unit
- ยป Iconic symbols
- General
- Related keyword lists
MitoPedia topics:
Enzyme