Difference between revisions of "Closed chamber"

Revision as of 09:57, 7 September 2020

Closed chamber

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

The O2k chamber can be used as a closed system or open system. Gas bubbles must be avoided.

Abbreviation: C

Closed chamber means that the experimental chamber and the capillary of the stopper are filled with liquid and no air space or bubble is present in the chamber with the stopper fully inserted into the volume-calibrated position. Any titrations are performed between closed states, but not during a closed state. For closing the chamber without a gas bubble, an excessive volume of liquid has to be present in the chamber (minimum 2.1 mL for the 2-mL chamber; 0.55 mL for the 0.5-mL chamber). The stirrer can be switched on. Any foam should be avoided. Before inserting the stopper, the capillary of the stopper must be free of aqueous medium. The conical shape of the standard stopper facilitates bubble-free closing of the chamber with stirrers switched on - the bubbles escape through the central capillary. After closing the chamber the excessive liquid must be aspirated from the receptacle of the stopper.

The procedure of bubble-free closing a chamber with a multisensor stopper is described in the manual for ion selective electrodes MiPNet15.03.

» Compare: Open chamber

Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Specific

O2k-Procedures

» High signal at zero oxygen

» POS calibration - static

» POS calibration - dynamic

MiPNet O2k-Procedures

» POS-calibration

» O2k-Manual: POS-service

» O2k-cleaning

» O2k-Series G and DatLab 6: Calibration

General

» Oxygen, dioxygen, O₂

» Oxygen calibration - DatLab

» Oxygen solubility

» Oxygen solubility factor

» Oxygen pressure

» Concentration

» Activity

» Pressure - Pascal

» Barometric pressure

» High-resolution respirometry

» OroboPOS

» Polarographic oxygen sensor

» MitoFit Quality Control System

» Sensitivity

Other keyword lists

» Keywords: Concentration and pressure

@@ Line 6: / Line 6: @@
 }}
-:::: '''Closed chamber''' means that the chamber and the capillary of the stopper is filled with liquid and no air space or bubble is present in the chamber. For closing the chamber without a bubble an excessive amount of liquid has to be present in the chamber (2.5 mL for 2 mL chamber). The conical shape of the standard stopper facilitates bubble-free closing of the chamber with stirrers switched on - the bubbles will escape through the capillary. If some bubble remains in the chamber, the capillary should be relieved by inserting long needle into the capillary. After closing the chamber the excessive liquid should be aspirated from the top of the stopper.
+:::: '''Closed chamber''' means that the experimental chamber and the capillary of the stopper are filled with liquid and no air space or bubble is present in the chamber with the stopper fully inserted into the volume-calibrated position. Any titrations are performed between closed states, but not during a closed state. For closing the chamber without a gas bubble, an excessive volume of liquid has to be present in the chamber (minimum 2.1 mL for the 2-mL chamber; 0.55 mL for the 0.5-mL chamber). The stirrer can be switched on. Any foam should be avoided. Before inserting the stopper, the capillary of the stopper must be free of aqueous medium. The conical shape of the standard stopper facilitates bubble-free closing of the chamber with stirrers switched on - the bubbles escape through the central capillary. After closing the chamber the excessive liquid must be aspirated from the receptacle of the stopper.
-:::: The procedure of  bubble-free closing closing  a chamber with a multisensor stopper is described in the manual for ion selective electrodes [[MiPNet15.03]].
+:::: The procedure of bubble-free closing a chamber with a multisensor stopper is described in the manual for ion selective electrodes [[MiPNet15.03]].
-:::: See also: [[Open chamber]]
+::::» ''Compare'': [[Open chamber]]
 {{Template:Keywords: Oxygen signal}}