Flux control ratio: Difference between revisions
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:::* In the DatLab 7.4 Excel template for oxygen flux analysis: | :::* In the DatLab 7.4 Excel template for oxygen flux analysis: | ||
:::: If the sample concentration is not yet known, the box ‘Known sample concentration’ can be unchecked, and the concentration will be considered by default as 1, with units [x·mL<sup>-1</sup>]. In this way, flux can be normalized and ''FCR''s can be obtained even if the sample concentration is unknown. | :::: If the sample concentration is not yet known, the box ‘Known sample concentration’ can be unchecked, and the concentration will be considered by default as 1, with units [x·mL<sup>-1</sup>]. In this way, flux can be normalized and ''FCR''s can be obtained even if the sample concentration is unknown. | ||
::::» ''Read also:'' [[Extensive quantity]] | ::::» ''Read also:'' [[Extensive quantity]] | ||
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=== ''FCR'' in DatLab plot === | === ''FCR'' in DatLab plot === | ||
:::* The entire plot of oxygen flux can be transformed to a ''FCR''. Click on 'Flux/Slope' in the pull-down menu. | :::* The entire plot of oxygen flux can be transformed to a ''FCR''. Click on 'Flux/Slope' in the DatLab pull-down menu. Select chamber A or B 'O2 slope'. Select 'Flux control ratio, FCR' and select the mark that corresponds to the reference state. Change the scale under 'Graph/Scaling' (or press F6). | ||
== References == | == References == |
Revision as of 10:07, 30 June 2020
Description
[[Description::Flux control ratios (FCR), are ratios of oxygen flux in different respiratory control states, normalized for maximum flux in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0 (0% and 100%).
For a given protocol or set of respiratory protocols, flux control ratios provide a fingerprint of coupling and substrate control independent of (i) mt-content in cells or tissues, (ii) purification in preparations of isolated mitochondria, and (iii) assay conditions for determination of tissue mass or mt-markers external to a respiratory protocol (CS, protein, stereology, etc.). FCR obtained from a single respirometric incubation with sequential titrations (sequential protocol; SUIT protocol) provide an internal normalization, expressing respiratory control independent of mitochondrial content and thus independent of a marker for mitochondrial amount. FCR obtained from separate (parallel) protocols depend on equal distribution of subsamples obtained from a homogenous mt-preparation or determination of a common mitochondrial marker.]]
Abbreviation: Has abbr::''FCR''
Reference: [[Info::Gnaiger 2014 MitoPathways, Gnaiger 2009 Int J Biochem Cell Biol, Doerrier 2018 Methods Mol Biol]]
Flux control factor: normalization of mitochondrial respiration
- » More details: Flux control factor
DatLab
Unknown sample concentration and normalization per unit sample [x]
- In the DatLab 7.4 Excel template for oxygen flux analysis:
- If the sample concentration is not yet known, the box ‘Known sample concentration’ can be unchecked, and the concentration will be considered by default as 1, with units [x·mL-1]. In this way, flux can be normalized and FCRs can be obtained even if the sample concentration is unknown.
- » Read also: Extensive quantity
- » More details: MiPNet24.06 Oxygen flux analysis - DatLab 7.4
FCR in DatLab plot
- The entire plot of oxygen flux can be transformed to a FCR. Click on 'Flux/Slope' in the DatLab pull-down menu. Select chamber A or B 'O2 slope'. Select 'Flux control ratio, FCR' and select the mark that corresponds to the reference state. Change the scale under 'Graph/Scaling' (or press F6).
References
{{#ask:Additional label::Flux control ratio | mainlabel=Bioblast link |?Has title=Reference |?Was published in year=Year |format=broadtable |limit=5000 |offset=0 |sort=Has title |order=ascending }}
- Bioblast links: Normalization - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Quantities for normalization
- » Count in contrast to Number
- » Mitochondrial marker
- » O2k-Protocols: mitochondrial and marker-enzymes
- » Citrate synthase activity
- Quantities for normalization
- General
- Related keyword lists
MitoPedia concepts:
Respiratory control ratio,
SUIT concept
MitoPedia methods:
Respirometry
MitoPedia O2k and high-resolution respirometry:
DatLab