COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping

MitoEAGLE protocols, terminology, documentation

WG1

Standard operating procedures and user requirement document: Protocols, terminology, documentation

WG1 Project application

Description

The quality management system, scope of SOPs to be elaborated and the outline of the data repositories will be defined in a user requirement document.

Respirometric reference protocols

A ‘library of protocols’ applied in mitochondrial respiratory physiology will be collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of reference protocols will rigorously document (Maelstrom Research program) the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating such bioenergetic tests (see also figure on the right).

Interlaboratory proficiency test

Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a reference OXPHOS protocol (Fig. 3). The organization of the proficiency test should follow the requirements of the ISO 17043 and 13528 standards. A corresponding MITOEAGLE proficiency training shall be developed and implemented. Feedback from the participants will lead to a final adjustment of the SOPs in the proficiency test, which will be made generally available.

Instrumental platforms

Comparison of results between and within instrumental platforms: The reference protocols and proficiency test will not define the instrumental platform, but will allow a quantitative comparison of results obtained with specific equipment available in the participating laboratories.

Nomenclature

Harmonization of nomenclature on mitochondrial respiratory states and control parameters: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MITOEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.

Knowledge management system

With an increase in data volume and complexity comes along the requirement for standardized report formats and digital accessibility of the data. Since such a database is still missing for mitochondrial physiology, the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. Databases will be established combining biological and clinical data comprising proprietary and publicly available datasets into one integrated solution. Data import and export functionalities will allow data upload and mapping to corresponding objects in the database, in this way generating valuable data and allowing for project-specific as well as cross-project data mining.

The ability to formulate meta-studies in our approach is an important success factor: The platform can aggregate the project’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental factors associated with disease prevention can be identified and aggregated into the MITOEAGLE Knowledge Management System.

Querying the heterogeneous data types and result representation are the most important requirements of the knowledge platform besides to data integration and modelling. The application will feature different types of visualization techniques for all integrated data types. The database will be developed using the concept of multi-tier client-server architecture. Individual components are separated corresponding to their functionality. An upstream web server handles user requests, an application server performing business logic, and a back-end database system guaranties persistent data storage. All components are additionally separated by means of network security incorporating firewalls and are hosted in a secure environment. The platform administration ensures privacy regarding proprietary data by user rights policies.

It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease.

Management

Tasks

Opening of the database to the research public for data search and moderated entry of new datasets.
Development of interlaboratory proficiency ring test, of recommendation/certification reports and of a MITOEAGLE proficiency training module.
Sample distribution, ring test experiments (MITOEAGLE-proficiency training on two different points of time, 6 months apart), data analysis by experimenters and MITOEAGLE-proficiency test manager.
Reports and publication: Reports with specific results in comparison with an anonymous data summary, and joint publication. Individual reports may be complemented by recommendations, and are summarized in an informal certification report.
Instrumental platform comparison with reference sample applying comparable measurements.
Draft, dissemination, collection and final formulation of a manuscript on concepts and terminology of mitochondrial physiology.
Collect and discuss procedures and experimental protocols for the evaluation of mitochondrial capacities and create a library of protocols: A database comprising reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis is assembled.
MITOEAGLE data management system (DMS) development: The DMS for further data preprocessing. The DMS will enable management and analysis of instrument-specific and project-specific data.
MITOEAGLE database (DB) development: DB will facilitate high-level project specific analysis as well as meta-analyses across projects, visualization of project specific data as well as integrative analyses of datasets for various proprietary and/or publicly available datasets.
Testing of developed software components regarding functionality and usability, modification and implementation. Deployment for routine testing in the labs of our strategic partners.
Data input: Upload of datasets generated through WG 2-4 in internal lab developments, results of the proficiency ring test, and during application module developments. In addition, the database will be populated with available proprietary and public datasets.
Develop recommendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation.
Provide a summary on strategic dissemination and an education programme for MITOEAGLE.

Milestones

Data sets entered in standardized format and database opened for the public
Protocols for ring-tests and standard analysis and report scheme defined
MITOEAGLE proficiency training workshop finished
Analysis is completed and joint publication submitted
Publication of paper on concepts and terminology
Library of protocols online
Publication of recommended protocols and procedures
Guidelines for acquisition, evaluation and data documentation available
MITOEAGLE data management system finished
MITOEAGLE database ready for use
Software modifications incorporated following extensive tests
Publication of MITOEAGLE-KMP
Recommendations on data acquisition, evaluation and management published
Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation published

Deliverables

Guidelines for future research and recommendations for the evaluation of respiratory characteristics in human respirometric samples
A database on mt fitness evaluated with human cells and tissues
Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation
Qualitative and quantitative evaluation of results obtained in ongoing studies by comparison with a reference sample
An expanded MITOEAGLE database
A joint publication documenting the results of the MITOEAGLE-PT
A multi-authored publication towards a unification of concepts and nomenclature in mitochondrial physiology
A library of protocols for sample preparation and examination by respirometry
A multi-authored publication with recommendations of comparable standard protocols and procedures.
Guidelines as to the optimum use of a reference sample for determination of respiration and related parameters of interest.
The MITOEAGLE data management system.
A continuously updated MITOEAGLE database for the use as reference data and guideline towards application of optimized protocols, ultimately leading towards standardization.
A joint publication on the use of the MITOEAGLE database and management system.
An education programme for the use of MITOEAGLE database and management system.

WG1 Action

Participants

Country	City	Contact
Armenia	Yerevan	Trchounian K
Australia	Queensland	Dong Lan-Feng
Australia	Melbourne, VIC	Muccini Anna Maria
Australia	Victoria	Van Bergen Nicole J
Australia	Parkville	Hardee Justin P
Australia	Melbourne	Ferri Alessandra
Australia	Queensland	Fisher Joshua J
Australia	Melbourne	Kuang Jujiao
Australia	Syndey	Ton Riccardo
Australia	Melbourne	Hewakapuge Sudinna
Australia	Nedlands	Filipovska Aleksandra
Australia	Melbourne	Lionett S
Australia	Clayton Victoria	Alton Lesley
Australia	Melbourne	Coughlan Melinda T
Australia	Sydney	Hoehn Kyle L
Australia	Melbourne	Cassar Samantha
Australia	Melbourne	Genders Amanda J
Australia	Queensland	Radenkovic Filip
Australia	Clayton	Wolff Jonci Nikolai
Australia	Melbourne	Saner Nicholas
Australia	Sydney	Krycer James R
Australia	Queensland	Holland Olivia J
Australia	Melobourne	Botella Javier
Australia	Victoria	Winwood-Smith Hugh
Australia	Nedlands	Perks Kara L
Australia	Southport	Vidimce Josif
Australia	Geelong, Victoria	McKenzie Matthew
Australia	Melbourne	Stepto Nigel K
Australia	Newtown, Sydney	Stocker Roland
Australia	Burwood	Trewin Adam J
Australia	Melbourne	Whitfield Jamie
Austria	Innsbruck	Antunes Diana
Austria	Innsbruck	Passrugger Manuela
Austria		Kapferer Werner
Austria	Salzburg	Kofler B
Austria	Innsbruck	Keller Markus A
Austria	Vienna	Kozlov Andrey V
Austria	Innsbruck	Cardoso Luiza HD
Austria	Innsbruck	Plangger Mario
Austria	Innsbruck	Nindl Veronika
Austria	Innsbruck	Thapa Maheshwor
Austria	Innsbruck	Burtscher Martin
Austria	Innsbruck	Schartner Melanie
Austria	Innsbruck	Goebel G
Austria	Innsbruck	Hoppel Florian
Austria	Innsbruck	Bergmeister Lisa
Austria	Innsbruck	Doerrier Carolina
Austria	Innsbruck	Di Marcello Marco
Austria	Innsbruck	Klocker Helmut
Austria	Innsbruck	Sobotka Ondrej
Austria	Innsbruck	Gallee Leon
Austria	Innsbruck	Karabatsiakis Alexander
Austria	Innsbruck	Perez Valencia Juan Alberto
Austria	Aldrans	Haider Markus
Austria	Innsbruck	Garcia-Souza Luiz F
Austria	Innsbruck	Schmitt Sabine
Austria	Innsbruck	Schwarzer Christoph
Austria	Innsbruck	Cecatto Cristiane
Austria	Klosterneuburg	Sazanov Leonid A
Austria	Innsbruck	Meszaros Andras T
Austria	Innsbruck	Laner Verena
Austria	Innsbruck	Gnaiger Erich
Belgium	Brussels	Sonveaux P
Belgium	Liege	Votion Dominique-Marie
Belgium	Namur	Arnould Thierry
Belgium	Leuven, Flanders	Baekelandt V
Belguim	Sart Tilman (Liège)	Kruse C
Brasil	Porto Alegre	Haas Clarissa
Brasilia	Limeira	Ropelle Eduardo R
Brazil	Jaboticabal	Oliveira Marcos Tulio
Brazil	Santa Maria - RS	Goncalves Debora Farina
Brazil	Rio de Janeiro	Oliveira Marcus F
Brazil	Santa Maria - Rio Grande do Sul	Soares Felix Alexandre Antunes
Brazil	Sao Paulo	Vercesi Anibal E
Brazil	Sao Paulo	Silber Ariel Mariano
Brazil	Rio de Janeiro	Galina Antonio
Brazil	São Paulo	Crispim Marcell
Brazil	São Paulo	Kowaltowski Alicia J
Brazil	Rio de Janeiro	Vieyra Adalberto
Canada	Guelph	Holloway Graham P
Canada	Winnipeg	Chowdhury Subir Roy
Canada	Montréal	Breton Sophie
Canada	Ottawa	Darveau Charles-A
Canada	Charlottetown	Adiele Reginald C
Canada	Hamilton	Scott Graham R
Canada	Rimouski	Blier Pierre U
Canada	Montreal, Quebec	Bergdahl Andreas
Canada	Ottawa	Harper Mary-Ellen
Canada	Saint John	Pulinilkunnil Thomas
Canada	Edmonton	Han Woo Hyun
Canada	Vancouver, BC	Tausan Daniel
Canada	Moncton, NB	Pichaud Nicolas
Canada	Quebec	Joseph Vincent
Canada	Edmonton (Alberta)	Lemieux Helene
Canada	Edmonton	Zaugg Michael
Canada	Rimouski	Munro Daniel
Canada	Antigonish	Weir M
Canada	Edmonton	Lucchinetti Eliana
Canada	Vancouver	Bovard Josh
Canada	Hamilton	Tarnopolsky Mark A
Chile	Coyhaique	Jana Prado Fabian
China	Xi`an	Liu Jiankang
China	Tianjin	Zhang Yong
China	Fuzhou, Fujian Province	Chen Qi
China	Pukou, Nanjing,	Gan Zhenji
Croatia	Zagreb	Rubelj Ivica
Croatia	Split	Ljubkovic M
Croatia	Zagreb	Saric A
Croatia	Zagreb	Curik I
Czech Republic	Pardubice	Rousar Tomas
Czech Republic	Prague	Drahota Zdenek
Czech Republic	Prague	Krizova J
Czech Republic	Prague	Houstek Josef
Czech Republic	Hradec Kralove	Endlicher Rene
Czech Republic	Ceske Budejovice	Zikova Alena
Czech Republic	Pardubice	Handl Jiri
Czech Republic	Praha 2	Danhelovska Tereza
Czech Republic	Hradec Kralove	Kucera Otto
Czech Republic	Vestec	Ezrova Zuzana
Czech Republic	Praha 2	Zdrazilova Lucie
Czech Republic	Vestec	Rohlena Jakub
Czech Republic	Plzen -Severni predmesti	Jedlicka Jan
Czech Republic	Vestec	Davidova Eliska
Czech Republic	Pardubice	Majtnerova Pavlina
Czech Republic	Hradec Kralove	Stankova Pavla
Czech Republic	Hradec Kralove	Cervinkova Zuzana
Czech Republic	Prague 10	Urban Tomas
Czech rep	Hradec Kralove	Falaye T
Denmark	Roskilde	Dalgaard Louise T
Denmark	Copenhagen	Gonzalez-Franquesa Alba
Denmark	Copenhagen	Kraunsoee R
Denmark	Copenhagen	Dela Flemming
Denmark	Copenhagen	Nielsen B
Denmark	Aarhus N	Boetker Hans Erik
Denmark	Copenhagen N	Mortensen OH
Denmark	Copenhagen	Larsen Steen
Denmark	Aarhus N	Jespersen Nichlas Riise
Denmark	Copenhagen	Soendergaard SD
Denmark	Copenhagen N	Maise Chroeis K
Denmark	Hvidovre	Nehlin Jan O
Denmark	Copenhagen N	Scheibye-Knudsen Morten
Egypt	Cairo	Ali Sameh S
Egypt	Cairo	Abdel-Rahman Engy Ali
Estonia	Tallinn	Karro N
Estonia	Tallinn	Puurand Marju
Estonia	Tartu	Paju Kalju
Estonia	Tallinn	Klepinin Aleksandr
Estonia	Tallinn	Laasmaa Martin
Estonia	Tallinn	Shevchuk Igor
Estonia	Tallinn	Truu Laura
Estonia	Tallinn	Kaambre Tuuli
Estonia	Tallinn	Klepinina Lyudmila
Estonia	Tallinn	Vendelin Marko
Estonia	Tallinn	Tepp Kersti
Finland	Jyvaeskylae	Kainulainen Heikki
Finland	Helsinki	Jackson Christopher Benjamin
Finland	Turku	Stier Antoine
Finland	Helsinki	Suomalainen Wartiovaara Anu
France	Montpellier	Wrutniak-Cabello Chantal
France	Angers	Procaccio Vincent
France	Paris	Armand Anne-Sophie
France	Bordeaux cedex	Mourier A
France	Maisons-Alfort Cedex	Prola Alexandre
France	Nouzilly	Collin-Chenot Anne
France	Bordeaux cedex	Rossignol Rodrigue
France	Lille	Montaigne David
France	Martinique	Neviere Remi
France	Martinique	Cano Sanchez Maria Consolacion
France	Bordeaux	Amoedo Nivea Dias
France	Paris	Bouillaud Frederic
France		Gueguen Naig
France	Plouzané	Salin Karine
France	Bordeaux	Sarlak Saharnaz
France	Tours	Dumas Jean-Francois
France	Angers	Spinazzi Marco
Germany	Regensburg	Renner-Sattler Kathrin
Germany	Mainz	Maull Felicia
Germany	Frankfurt	Osiewacz Heinz D
Germany	Neuherberg	Einer C
Germany	Gießen	Grewal Rekha
Germany	Biberach an der Riß	Nold V
Germany	Tuebingen	Kappler Lisa
Germany	Frankfurt	Warnsmann Verena
Germany	Wilhelmshaven	Salmon Pablo
Germany	Düsseldorf	Goy C
Germany	Ulm	Schaefer PM
Germany	Mainz	Methner Axel
Germany	Jena	Szibor Marten
Germany	Duesseldorf	Roden Michael
Germany	Giessen	Sommer Natascha
Germany	Munich	Zischka Hans
Germany	Magdeburg	Schoenfeld Peter
Germany	Marburg	Vogt Sebastian
Germany	Frankfurt	Hamann Andrea
Germany	Cologne	Wiesner Rudolf J
Germany		Wickert Anika
Germany	Duesseldorf	Jelenik T
Germany	Cologne	Ho Dieu Hien
Germany	Gießen	Silaidos Carmina
Germany	Giessen	Schulz Rainer
Germany	Marburg	Ramzan Rabia
Germany	Freiburg	Schuele R
Germany	Cologne	Pesta Dominik
Germany	Cologne	Trifunovic Aleksandra
Germany	Duesseldorf	Haendeler Judith
Germany	Hannover	Das Anibh Martin
Germany		Doermann Niklas
Germany	Frankfurt am Main	Viel Christian
Germany	Rostock	Sokolova Inna
Germany	Bremerhaven	Mark Felix Christopher
Germany	Cologne	Maciej Sarah
Germany	Duesseldorf	Piel Sarah
Greece	15771	Andreadou Ioanna
Greece	Athens	Gumeni Sentiljana
Greece	Thessaloniki	Lazou Antigone
Greece	Athens	Trougakos Ioannis P
Greece	Heraklion	Tavernarakis Nektarios
Hungary	Budapest	Tretter Laszlo
Hungary	Szeged	Ferdinandy Peter
Hungary	Szeged	Boros Mihaly
Hungary	Budapest	Komlodi Timea
Hungary	Budapest	Horvath G
Hungary	Debrecen	Verebne Tar K
Hungary	Budapest	Chinopoulos Christos
India	Kollam	Suravajhala Prashanth
India	New Delhi	Vincent Vinnyfred
India	Lucknow	Gayen Jiaur
India	Hyderabad	Jha Rajan Kumar
India	Haryana	Chakrabarti Sasanka
India	Varanasi	Sonkar Vijay K
India	New Delhi	Thakkar Himani
Iran	Tehran	Shirazi Reza
Iran	Tehran	Safaei Zahra
Ireland	Dublin	Porter Richard K
Ireland	Dublin	O'Gorman Donal
Israel	Jerusalem	Saada Reisch Ann
Israel	Beer Sheva	Mishmar Dan
Israel	Rishon Le Zion	Hachmo Yafit
Israel	Zrifin	Sova Marina
Italia	Udine	Salvadego Desy
Italien	Padova PD	Szabo Ildiko
Italy	Monserrato	Isola Raffaella
Italy	Bari	Attimonelli M
Italy	Milan	Clementi Emilio
Italy	Padova	Viscomi Carlo
Italy	Campobasso	Pallotta Maria Luigia
Italy	Catania	Messina Angela
Italy	Bologna	Genova Maria Luisa
Italy	Padova	Bernardi Paolo
Italy	Bolzano	Zanon Alessandra
Italy	Catania	De Pinto Vito
Italy	Rome	Scatena Roberto
Italy	Milano	Bottoni P
Italy	Ancona	Battino Maurizio
Italy	Padova	Fernandez-Vizarra Erika
Italy	Bologna	Paterlini S
Italy	Bologna	Porcelli AM
Italy	Catania	Magri Andrea
Italy	St. Lorenzen	Harrison David K
Italy	Udine	Grassi B
Italy	Bologna	Gasparre G
Italy	Bolzano/Bozen	Volani Chiara
Japan	Itabashi-ku Tokyo	Tanaka Masashi
Japan	Sapporo	Matsumoto J
Kitzbühel	Innsbruck	Fischer Michael J
Korea	Seoul	Pak Youngmi Kim
Korea	Incheon	Kwak Hyo Bum
Korea (South)	Seoul	Lee Hong Kyu
Latvia	Riga	Inashkina I
Latvia	Riga	Jansone Baiba
Latvia	Riga	Dambrova Maija
Latvia	Riga	Vilks Karlis
Latvia	Riga	Svalbe Baiba
Latvia	Riga	Zvejniece Liga
Latvia	Riga	Stelfa Gundega
Latvia	Riga	Liepins Edgars
Latvia	Riga	Volska Kristine
Luxembourg	Strassen	Devaux Yvan
Malaysia	Kuala Lumpur	Hassan Hazirah
Malta	Msida	Vella Joanna
Mexico	Mexico City	Aparicio Trejo OE
Mexico	Tlalpan	Rodriguez-Enriquez Sara
México	San Pedro Garza García, Nuevo Leon	Garcia-Rivas Gerardo
Netherlands	GA Wageningen	Grefte Sander
Netherlands	Maastricht	Grevendonk L
New Zealand	Auckland	Ward Marie Louise
New Zealand	Auckland	Kaur Sarbjot
New Zealand	Auckland	Hickey Anthony J
New Zealand	Wellington	Berridge Michael V
Norway	Bergen	Blindheim Dan Filip
Norway	Trondheim	Grill V
Norway	Bergen	Berge Rolf K
Norway	Bergen	Dyrstad Sissel E
Norway	Trondheim	Lerfall Joergen
Norway	Bergen	Haavik Jan
Norway	Trondheim	Smenes Benedikte Therese
Norway	Oslo	Eide L
Norway	Tromsoe	Boardman Neoma T
Norway	Trondheim	Rognmo O
Norway	Bergen	Pettersen Nitschke Ina Katrine
Norway	Bergen	Hoel Fredrik
Norway	Bergen	Tronstad Karl Johan
Norway	Bergen	Roesland Gro Vatne
Poland	Warsaw	Sek Aleksandra
Poland	Warsaw	Karkucinska-Wieckowska Agnieszka
Poland	Krakow	Dembinska-Kiec Aldona
Poland	Warsaw	Wieckowski Mariusz R
Poland	Poznan	Jarmuszkiewicz Wieslawa
Poland	Warsaw	Zablocki Krzysztof
Poland	Lodz	Watala Cezary
Poland	Warszawa	Jaskiewicz Anna
Poland	Warsaw	Dymkowska Dorota
Poland	Lodz	Labieniec-Watala Magdalena
Poland	Warsaw	Wasilewski M
Poland	Warsaw	Szewczyk Adam
Poland	Gdańsk	Kaczor Jan Jacek
Poland	Warsaw	Bednarczyk Piotr
Poland	Warsaw	Kampa Rafal Pawel
Portugal	Coimbra	Palmeira Carlos
Portugal	Coimbra	Duarte Filipe Valente
Portugal	Porto	Alves Marco G
Portugal	Coimbra	Rolo Anabela Pinto
Portugal	Porto	Crisostomo Luis
Portugal	Coimbra	Grilo Luis
Portugal	Porto	Silva Ana Maria
Portugal	Aveiro	Vitorino Rui Miguel Pinheiro
Portugal	Coimbra	Minuzzi Luciele M
Portugal	Lisboa	Macedo Maria Paula
Portugal	Aveiro	Ferreira Rita Maria P
Portugal	Porto	Oliveira Jorge
Portugal	Porto	Oliveira Pedro Fontes
Portugal	Cantanhede	Teodoro Joao Soeiro
Portugal	Cantanhede	Rodrigues Ana Sofia
Portugal	Porto	Moreira Bruno P
Portugal	Coimbra	Santos Diana
Portugal	Coimbra	Oliveira Paulo J
Portugal	Cantanhede	Amorim Ricardo
Portugal	Coimbra	Dias Candida
Portugal	Porto	Beleza Jorge
Qatar	Doha	Guarch Meritxell Espino
Republic of Ghana	Cape Coast	Tei BN
Republic of Korea	Daejeon	Park SH
Republic of Korea	Busan	Kim Hyoung Kyu
Republic of Korea	Busan	Han Jin
Republic of Korea	Yuseong-gu Daejeon	Quang Don T
Republic of Serbia	Novi Sad	Andric Silvana
Republic of Serbia	Belgrade	Krako Jakovljevic Nina
Republic of Serbia	Belgrade	Lalic Nebojsa M
Russia	Yoshkar-Ola	Dubinin M
Russia	Moscow	Pavlova Nadia
Russia	Moscow	Vinogradov Andrey D
Serbia	Belgrade	Djordjevic M
Serbia	Belgrade	Savkovic U
Serbia	Belgrade	Mandic M
Serbia	Belgrade	Misirkic Marjanovic Maja
Singapore	Singapore	Hausenloy Derek J
Singapore	Singapore	Singh Brijesh Kumar
Slovakia	Bratislava	Sumbalova Zuzana
Slovakia	Bratislava 45	Ferko Miroslav
Slovakia	Košice	Cizmarova Beata
Spain	Barcelona	Goncalo Teixeira da Silva Rui
Spain	Barcelona	Zorzano Antonio
Spain	Barcelona	Garcia-Roves Pablo Miguel
Spain	Sevilla	Bustos Matilde
Spain	A Coruña	Blanco FJ
Spain	Granada	Hidalgo-Gutierrez A
Spain	Madrid	Hernansanz-Agustin Pablo
Spain	Madrid	Moran M
Spain	Valencia	Casado Pinna Marta
Spain	Salamanca	Almeida Angeles
Spain	Barcelona	Gil J
Spain	Madrid	Aragones Lopez J
Spain	Barcelona	Gama Perez Pau
Spain	Armilla (Granada)	Fernandez-Ortiz Marisol
Spain	Las Palmas de Gran Canaria	Calbet Jose AL
Spain	Madrid	Rial Eduardo
Spain	Granada	Quiles JL
Spain	Barcelona	Perales Jose Carles
Spain	Barcelona	Bravo-Sagua Roberto
Spain	Barcelona	De la Torre Lara J
Spain	A Coruña	Mayan MD
Sweden	Stockholm	Larsen Filip J
Sweden	Lund	Ehinger Johannes K
Sweden	Stockholm	Nedergaard J
Sweden	Stockholm	Cardinale Daniele A
Sweden	Stockholm	Shabalina Irina G
Sweden	Gothenburg	Bhuvanachandran NS
Switzerland	Lausanne	Zanou Nadege
Switzerland	Lausanne	Lagarrigue Sylviane
Switzerland	Lausanne	Zalachoras I
Switzerland	Lausanne	Amati Francesca
Switzerland	Lausanne	Place Nicolas
Switzerland	Bern	Nuoffer Jean-Marc
Switzerland	Lausanne	Sandi Carmen
Switzerland	Lausanne	Gebara E
Switzerland	Zürich	Zaugg Kathrin
Switzerland	Lausanne	Burtscher Johannes
Switzerland	Bern	Wyss RK
Switzerland	Basel	Fornaro Mara
Switzerland	Zurich	Gorr Thomas A
Switzerland	Lausanne	Canto Alvarez Carles
Switzerland	Basel	Bouitbir Jamal
Switzerland	Lausanne	Morato Fornaguera L
Switzerland	Lausanne	Donnelly Chris
Switzerland	Lausanne	Weger M
Taiwan	Changhua City	Wei Yau-Huei
The Netherlands	Groningen	Bakker Barbara M
The Netherlands	BT Amsterdam	Wuest Rob CI
The Netherlands	Maastricht	Schrauwen Patrick
The Netherlands	Maastricht	Nabben Miranda
The Netherlands	Delft	McMillan Duncan GG
UK	London	Lane Nick
UK	London	Rodriguez Enrique
UK	Cambridge	Brown Guy C
UK	London	Bettinazzi Stefano
UK	Canterbury, Kent	Gourlay Campbell W
USA	Baltimore	Gonzalez-Freire Marta
USA	Rochester	Lanza Ian R
USA	Aurora	Sparagna Genevieve C
USA	El Paso	Bajpeyi Sudip
USA	Stanford	Stary Creed
USA	Durham	Li Pingan Andy
USA	Iowa City	Wagner Brett A
USA	Kansas City	Swerdlow Russell H
USA	Colorado Springs	Jacobs RA
USA	Fairbanks	Coker Robert H
USA	Corvallis	Robinson Matthew M
USA	Gainesville	Neyroud D
USA	Jupiter	Trivigno Catherine
USA	Orlando	Pino Maria F
USA	East Lansing	Zhang Yizhu
USA	Davis	Hellgren Kim T
USA	Fayetteville	Iyer Shilpa
USA	Little Rock	Borsheim Elisabet
USA	Little Rock	MacMillan-Crow Lee Ann
USA	Akron	Cohen Bruce H
USA	Baton Rouge	Irving Brian A
USA	La Jolla	Schilling Jan M
USA	Los Angeles	Kwast Kurt E
USA	Aurora	Moreau Kerrie
USA	Milwaukee	Kandel Sunil Mani
USA	Fayetteville	Rao RR
USA	Galveston	Durham William J
USA	Baton Rouge	Hand Steven C
USA	Ann Arbor	Tyrrell Daniel J
USA	Corvallis	Newsom Sean A
USA	Columbus	Sims Carrie A
USA	Philadelphia	Falk Marni J
USA	Kansas City	Thyfault John P
USA	Nashville	Wasserman David H
USA	Omaha	Kim Julian KS
USA	Little Rock	Parajuli Nirmala
USA	Portland	Garlid Keith D
USA	Greenville	Neufer P Darrell
USA	Indianapolis	Brozinick Joseph T
USA		Puskarich Michael A
USA	New York	Galkin Alexander
USA	Cleveland	Tandler Bernard
USA	Berkeley	Williams Caroline M
USA	Louisville	Menze Michael A
USA	Atlanta	Jang Young Charles
USA	La Jolla	Valentine Joseph Marco
USA	Winston-Salem	Ahn Bumsoo
USA	San Diego	Patel Hemal H
USA	Warminster	Orynbayeva Zulfiya
USA	St. Louis	Abumrad Nada A
USA	Cambridge	Wohlwend Martin
USA	Bethesda	Fessel Joshua Patrick
USA	Needham	Brown David A
USA	Stillwater	Davis Michael S
USA	Birmingham	Moellering Douglas R
USA	Miami	Fontanesi F
USA	Boston	Kristal Bruce S
USA	Philadelphia	Ganetzky Rebecca
USA	Pittsburgh	Jurczak Michael J
USA	San Diego; La Jolla	Molina Anthony JA
USA	Louisville	Skolik Robert A
USA	Wakefield	Varricchio Frederick
USA	Philadelphia	Jang David H
USA	Davis	Adams Sean H
USA	Fort Collins	Li Puma Lance C
USA	Provo	Hancock Chad R
USA	St. Louis	Pietka Terri A
USA	Winston-Salem	Tomar Dhanendra
USA	Pittsburgh	Prochownik Edward V
USA	Torrance	Rossiter Harry B
USA	East Lansing	Bazil Jason N
USA	Cleveland	Hoppel Charles L
USA	Aurora	Ford Ellen
USA	San Diego	Cannon Daniel T
USA	Fort Collins	Chicco Adam J
USA	New Haven	Cavalcanti-de-Albuquerque Joao Paulo
USA	Orlando	Sparks Lauren M
USA	Rochester	Nair K Sreekumaran
USA	Chicago	Dayanidhi Sudarshan
USA	Milwaukee	Dash Ranjan K
USA	Denton	Dzialowski Edward M
USA	Gainesville	Leeuwenburgh Christiaan
USA	Orlando	Coen Paul M
USA	Corvallis	Batterson Philip M
... further results

Task groups

TG leaders: suggestions discussed at the MitoFit Science Camp 2016 Kuehtai AT.

TG participants: listed as a summary from the feedback received.

TG1.1 Coupling states and coupling control ratios: Consensus statements

TG leaders: Renner-Sattler Kathrin DE / Gnaiger Erich AT / Hoppel Charles L US

TG participants:

TG1.2 SUIT protocols: nomenclature consistent with concept of database

TG leader: Doerrier Velasco Carolina A AT, Gnaiger Erich AT

TG participants: Attimonelli Marcella IT, Krako Nina RS

TG1.3 Experimental details in SUIT

TG leaders: Chicco Adam J US/ Hickey Anthony J NZ/ Doerrier Velasco Carolina A AT

TG participants: Casado Pinna Marta ES, Krako Nina RS

TG1.4 Instrumental platforms: comparisons

TG leaders: Elmer Eskil SE (suggested), Molina Anthony JA US

TG participants: Canto Carles CH (suggested), Genova Maria Luisa IT, Tronstad Karl Johan NO (suggested), Zorzano Antonio ES (suggested)

TG1.5 Knowledge management system

TG leaders: Drinnan Michael UK/ Plattner Christina AT

TG participants:

TG1.6 Proficiency test

TG leaders: Gnaiger Erich AT/ Doerrier Velasco Carolina A AT

TG participants: Genova Maria Luisa IT, Plattner Christina AT

TG1.7 Documentation

TG leaders: Arnould Thierry BE/ Votion Dominique-Marie BE

TG participants:

TG1.8 Translation to community in general

TG leader: Irving Brian A US

TG participants: Krako Nina RS

TG1.9 Dissemination Scientific community (formally WG5)

TG leaders: Makrecka-Kuka Marina LV / Gnaiger Erich AT

TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos ES, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE

TG1.10 Training, Short Term Scientific Missions (formally WG5)

TG leader: Klaus Susanne DE (suggested), Labieniec-Watala Magdalena PL (suggested)

TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos ES, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE

Comments and discussion

We are mostly interested in two WG: (1) Since we are working mostly with heart and muscles, we are interested in WG 2 - MITOEAGLE data repository in muscle and other tissues. (2) WG 1 - Standard operating procedures and user requirement document: Protocols, terminology, documentation. Since we have an expertise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabolism. Of course our parts of the WG are also in our interest. - Marina Makrecka-Kuka, Edgars Liepinsh (2016).
My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on skeletal and cardiac muscle and cultured cardiomyocytes and endothelial cells. I would particularly like to bridge the gap between the group leaders and young investigators, trying to represent the young people who are the active users of the OROBOROS equipment. This will fit in nicely with the goals in WG1. ... Major congratulations on obtaining this beautiful collaborative grant! Very glad to see this network getting the recognition it deserves! Looking forward to being part of this! - Rob C Wuest (2016).
Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types:
1. Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
2. Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
3. Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - Cezary Watala (2016).
I have already contacted the portuguese CNC to nominate me for the MC of theaction. Concerning my active participation, taking into account the type of work we do in my lab I could be included in all WGs. As you know we work with mitochondria from liver, muscle and fat, as well as cultured cells from different origins (WG2-4). The elaboration of SOPs (WG1) we can certainly contribute (see the book I edited on Mitochondrial Bioenergetics and in which you contribute a chapter). I believe that everybody can be part of the WG5, but as the coordinators of the action, you and Sandra should decide what is best for the success of the action. - Carlos Palmeira (2016).
I would propose my participation in the WG1: Standard operating procedures and user requirement document: Protocols, terminology, documentation, considering that the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. I could give my active contribution in the phase of making platform that will aggregate the Action’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases in particular tasks:
1. Develop reccomendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalised service for data management, data mining and health-care conforming standards of data interpretation
2. Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
3. Considering that we are in process of buying the oxygraph, thus broading capacities of my lab, I would be happy to open the door of my new lab for the innovative inter-laboratory ring test experiments and together with my team contribute in that phase of the MITOEAGLE Action. I look forward to the establishment of MitoGlobal EAGLE network where, as stated in the MoU, researchers will collaborate on mapping mitochondrial physiology and medicine. The creation of such a unique well-coordinated network of senior researchers and young investigators will definitely put an added value to existing data and publications, will increase quality and validity of the research, will bring new possibilities of data sharing and data mining and will hopefully bring us to the era of more economical research by saving resources through standardised and harmnonised protocols, where the measuremnts will not be wasted due to lack of comparability. - Nebojsa Lalic (2016).
Our research interest is oriented to reproductive endocrinology; especially to mechanisms that govern steroidogenesis of testicular Leydig cells in disturbed homeostasis. We use several in vivo models:
1. stress -/+ systemic or intratesticular blockade of different stress receptors (alpha1ADR; betaADR; GR)
2. hypogonadal-hypogonadism -/+ testosterone-replacement therapy
3. androgens anabolics treatment -/+ blockade od AR
4. aged rats -/+ Viagra-treatment
5. biological clock - Silvana Andric (2016).
I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation. Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining). - Pablo M Garcia-Roves (2016).
I will be pleased to contribute to the tasks of WG1 by developing interlaboratory proficiency tests and instrumental platform comparison. Moreover, as a (potential) MC member of MitoEAGLE I can also offer visibility to the Action itself (i.e. WG5: MITOEAGLE dissemination and training, according to mitoglobal.org webpages) by establishing new interactions with the International Coenzyme Q10 Association (ICQA) where I act as a member of the Executive Committee. ICQA organizes biennal conferences on new highlights of Coenzyme Q research. In 2015, we held the conference in Bologna (http://conference2015.wix.com/icqaconference) and we included in the program also some scientific sessions dedicated to mitochondrial bioenergetics and diseases. I will be pleased to enforce cooperation between ICQA and MitoEAGLE. - Maria Luisa Genova (2016).
We found the scientific content and objectives of the call very interesting and compatible with our wide expertise on oxysterol analysis in food and biological systems. Our group has a large experience on lipid composition and their degradation products (volatile and non-volatile), as well as on sensory analysis. Moreover, we are currently involved in the H2020-GERI-2014-1 project "PLOTINA" ("Promoting gender balance and inclusion in research, innovation and training") with Prof. Gallina Toschi (in cc) as coordinator; the project actually includes gendered case studies where gender and sex variables are considered. You can find further information about our expertises, publications and the PLOTINA project at the following webpages: https://www.unibo.it/sitoweb/maria.rodriguez/, https://www.unibo.it/sitoweb/tullia.gallinatoschi/, http://www.unibo.it/en/research/projects-and-initiatives/research-projects-horizon-2020-1/plotina - Maria Teresa Rodriguez Estrada (2016).
As you know, our current/developing interests are with "evolution" (species comparisons) and "environment" (hypoxia and cold exposure), but have also experience and interest in assessing effects of "lifestyle" (diet, exercise) and "aging" as well (in rodents). - Adam J Chicco (2016).
Congratulations on the considerable progress with MITOEAGLE! I am keen to participate in this exciting project and I’m sure I can make a valuable contribution to it's success. I have extensive experience performing respirometry assays in human muscle biopsies, myoblasts/tubes and mouse tissues in the contexts of aging, obesity, exercise and inactivity. I feel that I could contribute to any of the five working groups, but particularly to WG2, WG4, and WG5. I am also happy and willing to serve on the Management Committee if needed. Again, congratulations on organizing such an exciting project. - Paul M Coen (2016).
I would be interested to be involved with WG1 and WG2. In particular, I am interested in contributing to developing protocols for multicenter quality control among labs, and for permeabilized muscle bundle respirometry. - Harry B Rossiter (2016).