Description
Mitochondrial respiration medium, MitoOx2, developed for oxygraph incubations of mitochondrial preparations which yields a higher optical sensitivity for Amplex red.
Abbreviation: MitoOx2
MitoPedia methods:
Respirometry
MitoPedia topics:
Media for respirometry
Application in HRR
MitoOx2: Mitochondrial Respiration Medium
- Oxygen solubility factor of MitoOX2 at 30Β°C and 37Β°C = 0.95
- pH of MitoOx2: 7.0
Compound | Final conc. | FW | Company, product code and storage |
---|---|---|---|
KCl | 120 mM | Example | Example |
HEPES free acid | 20 mM | Example | Example |
KH2PO4 | 10 mM | Example | Example |
MgCl2 | 2.86 mM | Example | Example |
EGTA free acid | 380.35 | Example | Example |
BSA | 0.025% | Example | Example |