Difference between revisions of "O2k-OpenSupportAlert2020 Archive"

Revision as of 09:58, 4 May 2020

Currently Answered Questions from the »Oroboros Ecosystem Agenda«

May 2020

May-11 Th

Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.

- Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis. - »Steady state«

May-04 Mo

Do you load the correct instrumental background correction accordingly to your range of experimental O₂ levels?

- See how to perform the instrumental background test. - » O2 background correction in DatLab« and » Instrumental oxygen background«

Apr 2020

Apr-24 Fr

ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.

- »Hatefi 1962 J Biol Chem-XLII«

Apr-21 Tu

Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.

- »MiPNet17.04 CitrateSynthase«

Apr-13 Mo

Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?

- DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation - »SUIT-024«

Apr-08 We

The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.

- DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration. - »SUIT-010«

Apr-02 Th

Do you want to search the best SUIT protocol for your research questions? Use the SUITbrowser: »SUITbrowser«

Mar 2020

Mar-16 Mo

The standardized coupling control protocol to explore the bioenergetics of living cells

- DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - »SUIT-003«

Mar-11 We

Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2.

- »Blebbistatin«

Mar-06 Fr

Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.

- »RP1« and »RP2«

Mar-03 Tu

Rapamycin - more differences or more in common with other mitObesity drugs?

- »Rapamycin«

Feb 2020

Feb-26 We

It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity.

- »Noncoupled respiration«

Feb-24 Mo

More research is needed on the impact of viral infection on mitochondrial function.

- »Viruses and mitochondrial medicine«

Feb-19 We

Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function.

- »Spermidine«

Feb-18 Tu

Why do we fight gas bubbles?

- Dive deep into »Oxygen solubility«

Feb-12 We

What do metformin, melatonin and other bioactive mitObesity compounds have in common?

- »MitObesity drugs«

Jan 2020

Jan-16 Th

Oxygen flux

- which SI units should be used?

Did you ever think that units for body mass could be an issue?

- »Body mass«

Jan-08 We

Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.

@@ Line 5: / Line 5: @@
 * '''May-11 Th'''
 :{|
-| width="100" align="center" | [[Image:O2k-Support.jpg|80px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]]
+| width="100" align="center" | [[Image:O2k-Support.jpg|80px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
-|Do you know how to evaluate '''[[Fatty acid oxidation pathway control state|fatty acid oxidation (FAO, F-pathway)''']] avoiding to overestimate it?'''
+|'''Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.''' <br>
-Did you know that '''malate alone might support mt-respiration''' in [[Mitochondrial preparations| mitochondrial preparations]], if '''malate anaplerotic pathways''' ([[Malic enzyme|e.g. '''mt-malic enzyme''']]) are present? <br>
+- Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis.
-- Learn more about - »'''[[Malate anaplerotic pathway control state|malate anaplerotic pathway control state]]'''«<br>
+- »[[Steady state]]«
-- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«<br>
-- Since [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions, '''SUIT-002''' was specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«
 |}
 <br />
+* '''May-04 Mo'''
+:{|
+| width="100" align="center" | [[Image:O2k-Support.jpg|80px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
+|'''Do you load the correct instrumental background correction accordingly to your range of experimental O<sub>2</sub> levels?'''
+- See how to perform the instrumental background test.
+- »[[Flux / Slope| O2 background correction in DatLab]]« and »[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]«
+|}
 == Apr 2020 ==