Difference between revisions of "O2k-OpenSupportAlert2020 Archive"
From Bioblast
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== Jul 2020 == | |||
* '''Jul-27 Mo''' | |||
:{| | |||
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]] | |||
| '''Do you know how to prepare a homogenate from tissue samples using the [https://www.oroboros.at/index.php/product/pbi-shredder-o2k-set/ PBI-Shredder O2k-Set]?''' | |||
The PBI-Shredder O2k-Set provides a standardized approach to prepare homogenates of various tissues with high reproducibility and quality. <br> | |||
- Ā» [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: PBI-Shredder SG3 tissue homogenate preparation]Ā« | |||
|}Ā | |||
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* '''Jul-20 Mo''' | |||
:{| | |||
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]] | |||
|'''How can you compare respirometric experiments using an internal normalization?'''<br> | |||
Flux control ratios (''FCR'') are independent of external measurements (''e.g.'' protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the āO2 analysis template DL7.4ā. | |||
<br> | |||
- Ā»[[Flux control ratio]]Ā« | |||
Read more: Gnaiger Erich ''et al'' ā MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 Ā»[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]Ā« | |||
|} | |||
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* '''Jul-13 Mo''' | |||
:{| | |||
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]] | |||
|'''Did you know that you can apply instrumental O<sub>2</sub> background test parameters (''i.e.'', intercept and slope) from an existing file in DatLab?''' <br> | |||
In the DatLab file, you can copy the Instrumental O<sub>2</sub> background test parameters directly via the āCopy from fileā option in the āSlope configurationā window.Ā | |||
For more information, see: | |||
- Ā»[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Instrumental_O2_DLPĀ Copy from file]Ā« | |||
|} | |||
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* '''Jul-06 Mo''' | |||
:{| | |||
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] | |||
|'''Do you know what to do when the O2k-Peltier takes longer than 20 minutes to reach the set temperature? ''' <br> | |||
During [[Air calibration | air calibration]], temperature of the O2k-chamber and the O2k-Peltier power can be monitored under the layout '01 Calibration show Temp'. In case of an issue with the [[O2k-Peltier Temperature Control| temperature control]], an error message will appear. | |||
For more information, see: | |||
- Ā»[[O2k-Peltier_Mitopedia#Block_temperature_does_not_increase_while_heating_with_full_power|Block temperature does not increase while heating with full power]]Ā« and Ā»[[DatLab error messages]] Ā« | |||
|} | |||
== Jun 2020 == | == Jun 2020 == |
Revision as of 08:21, 3 August 2020
- Currently Answered Questions from the Ā»Oroboros Ecosystem AgendaĀ«
Aug 2020
- Aug-03 Mo
What do you do if the values for the āO2 slope backgroundā fields are not appearing correctly in the DatLab āMark statisticsā window?
It is possible to retrieve the values and correct units displayed in the Mark Statistics window by updating the Windows control panel settings.
- Ā» Troubleshooting for Mark Statistics windowĀ«