Octanoylcarnitine: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=Oct | |abbr=Oct | ||
|description=''' | |description='''Octanoylcarnitine''' is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to [[carnitine]], frequently applied as a substrate for [[fatty acid oxidation]] (FAO) in [[mitochondrial preparations]]. | ||
|info=http://www.jbc.org/content/256/16/8371.full.pdf ''pdf'' | |info=[http://www.jbc.org/content/256/16/8371.full.pdf ''pdf''] | ||
}} | }} | ||
{{MitoPedia concepts}} | |||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Substrate and metabolite | |||
}} | |||
:Ā» [[Fatty acid oxidation]] | |||
:Ā» [[Carnitine O-octanoyltransferase]] | |||
__TOC__ | |||
== Application in [[HRR]] == | |||
{{Chemical_description | |||
|abbr=Oct | |||
|trivial name=Octanoylcarnitine | |||
|complete name=(Ā±)-Octanoylcarnitine chloride | |||
|chem formula=C<sub>15</sub>H<sub>30</sub>NO<sub>4</sub>Cl | |||
|molar mass=323.86 | |||
|vendor=BioTrend (brand: APExBIO Technology) | |||
|product number=B6371-50 mg | |||
|store at=-20 Ā°C | |||
|sensitivity= | |||
|cas=14919-35-8 | |||
|h statements= | |||
|h info= | |||
}}<!--:::: '''Oct: Octanoylcarnitine''' ((Ā±)-Octanoylcarnitine chloride, C<sub>15</sub>H<sub>30</sub>NO<sub>4</sub>Cl); BioTrend (brand: APExBIO Technology) Cat. No. B6371, 50 mg, store at -20Ā°C; M = 323.86 gĀ·mol<sup>-1</sup>.--> | |||
:::: '''Alternative:''' <!--(Ā±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; M = 323.86 gĀ·mol<sup>-1</sup>.--> TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); M = 323.85 gĀ·mol<sup>-1</sup>. | |||
:::: '''General tips:''' For obtaining a higher solubility, warm the tube at 37 ā and shake it in the ultrasonic bath for a while. | |||
:::: '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O or DMSO, can differ from batch to batch - check Certificate of Analysis): | |||
::::# Weigh 50 mg of octanoylcarnitine and dissolve in 1543 ĀµL H<sub>2</sub>O or DMSO. | |||
::::# Divide into 0.2 mL portions. | |||
::::# Store at -20 Ā°C. | |||
:::::* '''''Note 2024-03-26:''''' According to the information provided by the supplier (BioTrend) is the solubility of Octanoylcarnitine in DMSO and H<sub>2</sub>O comparable ( ā„36.9 mg/mL in DMSO; ā„18.6 mg/mL in EtOH; ā„37.4 mg/mL in H<sub>2</sub>O), therefore we consider more rational approach to use H<sub>2</sub>O as a solvent in OROBOROS Lab because of better handling at room temperature. Disadvantage of DMSO: freezing point of +18.55 Ā°C (65.4 Ā°F). When using DMSO as solvent, make sure to bring the stock solution to room temperature before filling the syringe, which is important to avoid blocking the needle. | |||
:::::: communicated by [[User:Sobotka Ondrej|Sobotka Ondrej]] ([[User talk:Sobotka Ondrej|talk]]) 17:46, 26 March 2024 (CET) | |||
:::Ā» '''O2k manual titrations into 2 mL O2k-chamber''' | |||
::::* Titration volume ('''2-mL O2k-chamber'''): 10 ĀµL using a 25 ĀµL Hamilton syringe. | |||
::::* Titration volume ('''0.5-mL O2k-chamber'''): 2.5 ĀµL using a 10 ĀµL Hamilton syringe | |||
::::* Final concentration: 0.5 mM in [[MiR05-Kit|MiR05]] or [[MiR06]]. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05. | |||
{{Labeling | {{Labeling | ||
| | |additional=MitoPedia:FAT4BRAIN | ||
}} | }} |
Latest revision as of 10:59, 4 April 2024
Description
Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.
Abbreviation: Oct
Reference: pdf
MitoPedia topics: Substrate and metabolite
Application in HRR
- Oct: Octanoylcarnitine ((Ā±)-Octanoylcarnitine chloride; C15H30NO4Cl), BioTrend (brand: APExBIO Technology): B6371-50 mg, store at -20 Ā°C, CAS: 14919-35-8, M = 323.86 gĀ·mol-1
- Alternative: TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); M = 323.85 gĀ·mol-1.
- General tips: For obtaining a higher solubility, warm the tube at 37 ā and shake it in the ultrasonic bath for a while.
- Preparation of 100 mM stock solution (dissolved in H2O or DMSO, can differ from batch to batch - check Certificate of Analysis):
- Weigh 50 mg of octanoylcarnitine and dissolve in 1543 ĀµL H2O or DMSO.
- Divide into 0.2 mL portions.
- Store at -20 Ā°C.
- Note 2024-03-26: According to the information provided by the supplier (BioTrend) is the solubility of Octanoylcarnitine in DMSO and H2O comparable ( ā„36.9 mg/mL in DMSO; ā„18.6 mg/mL in EtOH; ā„37.4 mg/mL in H2O), therefore we consider more rational approach to use H2O as a solvent in OROBOROS Lab because of better handling at room temperature. Disadvantage of DMSO: freezing point of +18.55 Ā°C (65.4 Ā°F). When using DMSO as solvent, make sure to bring the stock solution to room temperature before filling the syringe, which is important to avoid blocking the needle.
- communicated by Sobotka Ondrej (talk) 17:46, 26 March 2024 (CET)
- Ā» O2k manual titrations into 2 mL O2k-chamber
- Titration volume (2-mL O2k-chamber): 10 ĀµL using a 25 ĀµL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 2.5 ĀµL using a 10 ĀµL Hamilton syringe
- Final concentration: 0.5 mM in MiR05 or MiR06. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.
Labels:
MitoPedia:FAT4BRAIN