Octanoylcarnitine: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
:::: '''Oct: Octanoylcarnitine''' ((±)-Octanoylcarnitine chloride, C<sub>15</sub>H<sub>30</sub>NO<sub>4</sub>Cl); APExBIO Technology | :::: '''Oct: Octanoylcarnitine''' ((±)-Octanoylcarnitine chloride, C<sub>15</sub>H<sub>30</sub>NO<sub>4</sub>Cl); BioTrend (brand: APExBIO Technology) Cat. No. B6371, 50 mg, store at RT; M = 323.86 g·mol<sup>-1</sup>. | ||
:::: '''Alternative:''' <!--(±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; M = 323.86 g·mol<sup>-1</sup>.--> TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); M = 323.85 g·mol<sup>-1</sup>. | |||
:::: '''Alternative:''' (±)-Octanoylcarnitine chloride, BioTrend, Cat. No. BS0136, 50 mg, store desiccate at RT; M = 323.86 g·mol<sup>-1</sup>. | |||
:::: '''General tips:''' For obtaining a higher solubility, warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. | :::: '''General tips:''' For obtaining a higher solubility, warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. | ||
Revision as of 10:41, 22 March 2021
- high-resolution terminology - matching measurements at high-resolution
Octanoylcarnitine
Description
Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.
Abbreviation: Oct
Reference: pdf
Application in HRR
- Oct: Octanoylcarnitine ((±)-Octanoylcarnitine chloride, C15H30NO4Cl); BioTrend (brand: APExBIO Technology) Cat. No. B6371, 50 mg, store at RT; M = 323.86 g·mol-1.
- Alternative: TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); M = 323.85 g·mol-1.
- General tips: For obtaining a higher solubility, warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
- Preparation of 100 mM stock solution (dissolved in H2O or DMSO, can differ from batch to batch - check Certificate of Analysis):
- Weigh 50 mg of octanoylcarnitine and dissolve in 1543 µL H2O or DMSO.
- Divide into 0.2 mL portions.
- Store at -20 °C.
- » O2k manual titrations into 2 mL O2k-chamber
- Titration volume (2-mL O2k-chamber): 10 µL using a 25 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 2.5 µL using a 10 µL Hamilton syringe
- Final concentration: 0.5 mM in MiR05 or MiR06. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.
MitoPedia topics: Substrate and metabolite
