Oroboros FAQ: Difference between revisions
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== Applications and samples == | == Applications and samples == | ||
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<span style="font-size:105%; color:#424242">'''Can the O2k be applied to measure tissue oxygen concentration?'''</span> | <span style="font-size:105%; color:#424242">'''Can the O2k be applied to measure tissue oxygen concentration?'''</span> | ||
<div class="mw-collapsible-content"> No. The O2k is a [[O2k- | <div class="mw-collapsible-content"> No. The O2k is a [[O2k-chamber|'chamber']] approach, for application with tissue biopsies, cultured cells or [[Isolated_mitochondria|isolated mitochondria]]. Whereas HRR cannot be applied for measurement of tissue O<sub>2</sub> concentration, it is the method of choice for analysis of tissue O<sub>2</sub> consumption and functional diagnosis of mitochondrial injuries.</div> | ||
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<span style="font-size:105%; color:#424242">'''Can the O2k be applied for measurement of oxygen consumption in [[living cells|adherent cells]]?'''</span> | <span style="font-size:105%; color:#424242">'''Can the O2k be applied for measurement of oxygen consumption in [[living cells|adherent cells]]?'''</span> | ||
<div class="mw-collapsible-content"> Attached cells may be grown on disks before being introduced into the [[O2k- | <div class="mw-collapsible-content"> Attached cells may be grown on disks before being introduced into the [[O2k-chamber|cylindrical chamber]] of the O2k. Alternatively, cells attached to beads can be used. The [[Sample Holder]] may be useful for these applications. Alternatively, gentle stirring, with the use of specific small [[Stirrer-Bar\white PVDF\15x6 mm|stirring bars]], is advisable to avoid crashing of the beads. Routinely, we apply the O2k on adherent cells after transfering them into suspension. For small cells, such as fibroblasts, at least 200,000 cells per chamber are required. | ||
More information: [[Zdrazilova 2022 PLOS ONE]]</div> | |||
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<span style="font-size:105%; color:#424242">'''How many parameters can be measured simultaneously?'''</span> | <span style="font-size:105%; color:#424242">'''How many parameters can be measured simultaneously?'''</span> | ||
<div class="mw-collapsible-content"> The O2k is designed to measure respiration plus one additional parameter at a time. | <div class="mw-collapsible-content"> The O2k is designed to measure respiration plus one additional parameter at a time. It might be possible to measure more than one additional parameter at a time with the O2k-Q-Module and O2k-Fluo Smart-Module. | ||
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== Delivery content, warranty and support == | == Delivery content, warranty and support == | ||
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Latest revision as of 11:36, 8 November 2023
Oroboros FAQ
Applications and samples
Is the O2k easy to use in standard applications of mitochondrial respiration?
Most 'oxygraphs' are designed for analyzing isolated mitochondria. Which types of samples can be analyzed with the O2k?
Can the O2k be applied for the measurement of oxygen consumption in delicate mammalian cells, when limited numbers of cells are available?
Can the O2k be applied for measuring human biopsies?
Can the O2k be applied to measure tissue oxygen concentration?
Can the O2k be applied for measurement of oxygen consumption in adherent cells?
How many parameters can be measured simultaneously?
Delivery content, warranty and support
Does the purchase include installation, setup and training?
- Installation and setup
On-site installation and setup is not included in the purchase of an Oroboros O2k. Setup and installation are usually performed by the customers themselves with the help of our detailed and user-friendly O2k-Manual which is sent out with the O2k. In addition, the manual can also be easily down-loaded form our website (O2k-Manual). There is also a section for technical service. Our technical specialists, based here in Innsbruck, can also assist in helping to locate and solve any technical problems, should these arise. Based on our experience and feedback received from customers all over the world, we are confident that you will easily master the set-up and installation.
- Training
Is it possible to extend the warranty period?
Yes, it is possible to extend the warranty period by a total of three years. For more details, please contact our office: [email protected]
Do you offer the option to lease or rent your instruments?
It is possible to lease the O2k over the course of three years, after which the instrument passes over into your ownership. Furthermore, you can rent an instrument for a short period (e.g. several weeks or months) if you need to improve your throughput for a specific study or experiment. Contact our office for further details: [email protected]
Are you registered with System for Award Management (SAM)?
Yes.