Oroboros FAQ: Difference between revisions
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High-resolution respirometry is the method of choice when aiming at investigating mitochondrial function either in intact cells or various types of mitochondrial preparation. Combining instrumental accuracy and reliability with versatility of applicable protocols - allowing practically unlimited addition and combination of substrates, inhibitors and uncouplers - mitochondrial respiratory pathways may be analyzed in detail to evaluate even minor alterations in respiratory pathway control and/or capacity. ย | High-resolution respirometry is the method of choice when aiming at investigating mitochondrial function either in intact cells or various types of mitochondrial preparation. Combining instrumental accuracy and reliability with versatility of applicable protocols - allowing practically unlimited addition and combination of substrates, inhibitors and uncouplers - mitochondrial respiratory pathways may be analyzed in detail to evaluate even minor alterations in respiratory pathway control and/or capacity. ย | ||
The O2k is a sole source apparatus, with no other available instrument meeting its specifications for high-resolution respirometry. ย | The O2k is a sole source apparatus, with no other available instrument meeting its specifications for high-resolution respirometry. ย | ||
'''[[High-resolution respirometry]] (HRR)''' is based on the O2k, combining chamber design, application of oxygen-tight materials, electrochemical and optical sensors, Peltier-temperature control and software features [[DatLab|(DatLab)]] to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with both, a closed- and open-chamber mode of operation. | |||
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Revision as of 11:47, 17 March 2019
Oroboros FAQ
Applications and samples
The O2k is used in basic and applied research to study the respiration of tissues, cells and mitochondria. Fairly simple to highly complex, comprehensive experimental protocols can be applied to assess either basal mitochondrial characteristics or obtain diagnostic insight into respiratory pathways. Topically, studies using the O2k range from comparative physiological investigations addressing evolutionary and environmental aspects of mitochondrial metabolism over the energetics related to exercise physiology, nutrition and life style, to aspects of clinical interest such as the impact of metabolic diseases, cancer, or pharmacological interventions.
- The most prominent applications of the O2k
- Studies on exercise physiology;nutrition;life style
- Diagnosis of acquired and genetic mitochondrial diseases
- Diagnosis of myopathies and neuromuscular pathologies
- Studies on cell function and cell death - apoptosis and necrosis
- Measurement of oxidative stress
- Studies on ischemia-reperfusion injury
- Studies on aging and senescence
- Pharmacological tests
- Studies on Diabetes 2
- For more applications, browse through the 4710 O2k-Publications in the MiPMap.
What is high-resolution respirometry and what is so special about it?
High-resolution respirometry is the method of choice when aiming at investigating mitochondrial function either in intact cells or various types of mitochondrial preparation. Combining instrumental accuracy and reliability with versatility of applicable protocols - allowing practically unlimited addition and combination of substrates, inhibitors and uncouplers - mitochondrial respiratory pathways may be analyzed in detail to evaluate even minor alterations in respiratory pathway control and/or capacity. The O2k is a sole source apparatus, with no other available instrument meeting its specifications for high-resolution respirometry. High-resolution respirometry (HRR) is based on the O2k, combining chamber design, application of oxygen-tight materials, electrochemical and optical sensors, Peltier-temperature control and software features (DatLab) to obtain the unique sensitive and quantitative resolution of oxygen concentration and oxygen flux, with both, a closed- and open-chamber mode of operation.
Is the O2k easy to use in standard applications of mitochondrial respiration?
Most 'oxygraphs' are designed for analyzing isolated mitochondria. Which types of samples can be analyzed with the O2k?
Can the O2k be applied for the measurement of oxygen consumption in delicate mammalian cells, when limited numbers of cells are available?
Can the O2k be applied for measuring human biopsies?
Can the O2k be applied to measure tissue oxygen concentration?
Can the O2k be applied for measurement of oxygen consumption in adherent cells?
How many parameters can be measured simultaneously?
Software compatibility
O2k in comparison with other devices
How and where to buy
- We cooperate with national distributors in the following countries and regions:
- North China, Hongkong Region, Macao Region: CN Beijing Huawei
- South China: CN Shanghai Zenda
- Taiwan: TW Taoyuan Sunpoint
- Republic of Korea: KR Seoul Scitech Korea
- Japan: JP Tokyo Berthold
- In all other countries, Oroboros Instruments has a direct-sales approach: we provide direct service and distribute our products directly to the end user. If required by the end user, it is possible to cooperate with a distributor.
How do I obtain price information on the O2k?
Are taxes and duty fees included in the price?
No, our offer does not include any taxes or any applicable duty fees. Please consider this when budgeting the purchase.
Whom do I contact to buy an O2k
Delivery content, warranty and support
Does the purchase include installation, setup and training?
- Installation and setup
On-site installation and setup is not included in the purchase of an Oroboros O2k. Setup and installation are usually performed by the customers themselves with the help of our detailed and user-friendly O2k-Manual which is sent out with the O2k. In addition, the manual can also be easily down-loaded form our website (O2k-Manual). There is also a section for technical service. Our technical specialists, based here in Innsbruck, can also assist in helping to locate and solve any technical problems, should these arise. Based on our experience and feedback received from customers all over the world, we are confident that you will easily master the set-up and installation.
- Training
Does the O2k-Core include a PC or do we have to purchase it separately?
No, the purchase of an O2k does not include a PC. However, a laptop or a PC (PC Requirements) is required to connect the O2k and run the required software (DatLab).
Is it possible to extend the warranty period?
Yes, it is possible to extend the warranty period by a total of three years. For more details, please contact our office: [email protected]
Do you offer the option to lease or rent your instruments?
It is possible to lease the O2k over the course of three years, after which the instrument passes over into your ownership. Furthermore, you can rent an instrument for a short period (e.g. several weeks or months) if you need to improve your throughput for a specific study or experiment. Contact our office for further details: [email protected]
Are you registered with System for Award Management (SAM)?
Yes.