Difference between revisions of "Respiratory state"
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::: Coupling control states and ''[[CCR]]'' of mitochondrial preparations: | ::: Coupling control states and ''[[CCR]]'' of mitochondrial preparations: | ||
::::* [[OXPHOS]], [[LEAK]], [[Electron transfer pathway|ET-pathway]] (''P, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[P/E]]'', ''[[L/P]]'' | ::::* [[OXPHOS]], [[LEAK respiration]], [[Electron transfer pathway|ET-pathway]] (''P, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[P/E]]'', ''[[L/P]]'' | ||
::: Coupling control states of living cells: | ::: Coupling control states of living cells: | ||
::::* [[ROUTINE]], [[LEAK]], [[Electron transfer pathway|ET-pathway]] (''R, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[R/E]]'', ''[[L/R]]'' | ::::* [[ROUTINE]], [[LEAK respiration]], [[Electron transfer pathway|ET-pathway]] (''R, L, E'') - corrected for [[ROX]]. ''CCR'': ''[[L/E]]'', ''[[R/E]]'', ''[[L/R]]'' | ||
== Electron-transfer-pathway state == | == Electron-transfer-pathway state == |
Revision as of 12:20, 25 November 2020
Description
Respiratory states of mitochondrial preparations and living cells are defined in the current literature in many ways and with a diversity of terms. Mitochondrial respiratory states must be defined in terms of both, the coupling-control state and the electron-transfer-pathway state.
Reference: Gnaiger 2014 MitoPathways
MitoPedia concepts:
MiP concept,
Respiratory state,
SUIT concept,
Recommended
MitoPedia methods:
Respirometry,
Spectrophotometry
MitoPedia topics:
EAGLE
Communicated by Gnaiger E 2010-10-21, edited 2016-08-26. Edited by Doerrier C 2020-04-23.
Coupling control states
- Coupling control states and CCR of mitochondrial preparations:
- OXPHOS, LEAK respiration, ET-pathway (P, L, E) - corrected for ROX. CCR: L/E, P/E, L/P
- Coupling control states of living cells:
- ROUTINE, LEAK respiration, ET-pathway (R, L, E) - corrected for ROX. CCR: L/E, R/E, L/R
Electron-transfer-pathway state
- Electron-transfer-pathway state, are defined by substrate type (at saturating concentration):
- Living cells: endogenous, exogenous substrate control
- Mitochondrial preparations: specific substrate-inhibitor combinations for selectively stimulating electron entry though Complex I (NADH Electron transfer pathway state), CII (succinate pathway), or other branches converging at the Q-junction, particularly with fatty acid oxidation (fatty acid oxidation pathway control state), alpha-glycerophosphate, or substrate combinations applied for reconstitution of TCA cycle function (e.g. NS-pathway control state, etc.).
- Control by substrate concentration: Kinetic control states:
- Kinetic substrate or adenylate control: Kinetic studies with variation of a specific substrate (reduced substrate supplying electrons to the ETS; ADP, Pi; O2; cytochrome c) are analyzed by kinetic functions (e.g. hyperbolic), yielding apparent kinetic constants, such as Jmax, Km', c50, or p50.
- Kinetic inhibitor control: Kinetic studies with variation of a specific inhibitor yield apparent kinetic constants, such as the KI'.
Classical respiratory states
- Chance and Williams (1955):
- Derived respiratory states:
- Thermodynamics of irreversible processes:
MitoPedia: Respiratory states
- Β» See the complete MitoPedia: Respiratory states
- Β» MitoPedia: SUIT