From Bioblast
Description
Abbreviation: FNS(PalOct,PGM)
Reference: A Β»Versions
- SUIT-category: FNS(PalOct,PGM)
- SUIT protocol pattern: diametral 1PalM;2D;3Oct;4P;5G;6U;7S;8Rot-
SUIT-019 gives information on F-pathway in LEAK and OXPHOS states. Moreover, this SUIT protocol allows the pathway control evaluation of FN in OXPHOS state and FN, FNS and S in ET state.
Communicated by Cardoso LH Doerrier C, Komlodi T and Gnaiger E (last update 2019-02-12)
Specific SUIT protocols
- SUIT-019 O2 pfi D045 for permeabilized fibers
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1PalM | PalML(n) | F(N) | CETF | 1PalM
|
2D | PalMP | F(N) | CETF | 1PalM;2D
|
2c | PalMP | F(N) | CETF | 1PalM;2D;2c
|
3Oct | PalOctMP | F(N) | CETF | 1PalM;2D;2c;3Oct
|
4P | PalOctPMP | FN | CETF&CI | 1PalM;2D;2c;3Oct;4P
|
5G | PalOctPGMP | FN | CETF&CI | 1PalM;2D;2c;3Oct;4P;5G
|
6U | PalOctPGME | FN | CETF&CI&II | 1PalM;2D;2c;3Oct;4P;5G;6U
|
7S | PalOctPGMSE | FNS | CI&CII | 1PalM;2D;2c;3Oct;4P;5G;6U;7S
|
8Rot | SE | S | CII | 1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot
|
9Ama | ROX | 1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot;9Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
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- Coupling control
- Pathway control
- Β» Electron transfer pathway
- Β» Fatty acid oxidation pathway control state, F
- Β» NADH electron transfer-pathway state, N
- Β» Succinate pathway control state, S
- Β» NS-pathway control state, NS
- Β» Glycerophosphate pathway control state, Gp
- Β» Complex IV single step, CIV
- Β» Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- Β» Glutamate, G
- Β» Glycerophosphate, Gp
- Β» Malate, M
- Β» Octanoylcarnitine, Oct
- Β» Pyruvate, P
- Β» Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- SUIT-019 allows the study of OXPHOS capacity in the fatty acid oxidation (F) and fatty acid oxidation&NADH pathway (FN) control states.
- + The protocol provides information on F-pathway (in presence of palmitoylcarnitine, and palmitoylcarnitine&octanoylcarnitine) in LEAK and OXPHOS states. In human heart muscle, the addition of octanoylcarnitine to palmitoylcarnitine&malate increased OXPHOS by 26% (Lemieux H et al 2011).
- + SUIT-019 provides information of the linear coupling (L-P) in FN-pathway.
- + Maximum ET capacity in FNS-pathway is obtained in SUIT-019.
- + P/E for FN-pathway (5G/6U) can be calculated.
- + Reasonable duration of the experiment.
- - Octanoylcarnitine and palmitoylcarnitine can have an uncoupling effect on OXPHOS state if their concentration is not optimized.
- - In the presence of anaplerotic pathways F-pathway is overestimated.
- - CIV activity is not measured, to save experimental time.
Compare SUIT protocols
- SUIT-002 (RP2): SUIT protocol specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways.
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Lemieux 2011 Int J Biochem Cell Biol | 2011 | Lemieux H, Semsroth S, Antretter H, HΓΆfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729β38. https://doi.org/10.1016/j.biocel.2011.08.008 | Human | Heart |
MitoPedia concepts:
MiP concept,
SUIT protocol,
Recommended
MitoPedia methods:
Respirometry