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SUIT-019
Description
Abbreviation: FNS(PalOct,PGM)
Reference: A Β»Versions
- SUIT-category: FNS(PalOct,PGM)
- SUIT protocol pattern: diametral 1PalM;2D;3Oct;4P;5G;6U;7S;8Rot
DLP applications
References
Steps and respiratory states
Step
|
State
|
Pathway
|
Q-junction
|
Comment - Events (E) and Marks (M)
|
1PalM
|
PalML(n)
|
F(N)
|
CETF
|
1PalM
- Respiratory stimulation of the FAO-pathway, F, by fatty acid FA in the presence of malate M. Malate is a type N substrate (N), required for the F-pathway. In the presence of anaplerotic pathways (e.g., mitochondrial malic enzyme, mtME) the F-pathway capacity is overestimated, if there is an added contribution of NADH-linked respiration, F(N) (see SUIT-002). The FA concentration has to be optimized to saturate the FAO-pathway, without inhibiting or uncoupling respiration.
- Non-phosphorylating resting state (LEAK state); LEAK respiration L(n) in the absence of ADP, ATP, AMP (no adenylates).
|
2D
|
PalMP
|
F(N)
|
CETF
|
1PalM;2D
|
2c
|
PalMP
|
F(N)
|
CETF
|
1PalM;2D;2c
- Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]).
- Respiratory stimulation of the FAO-pathway, F, by fatty acid FA in the presence of malate M. Malate is a type N substrate (N), required for the F-pathway. In the presence of anaplerotic pathways (e.g., mitochondrial malic enzyme, mtME) the F-pathway capacity is overestimated, if there is an added contribution of NADH-linked respiration, F(N) (see SUIT-002). The FA concentration has to be optimized to saturate the FAO-pathway, without inhibiting or uncoupling respiration.
- OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
|
3Oct
|
PalOctMP
|
F(N)
|
CETF
|
1PalM;2D;2c;3Oct
|
4P
|
PalOctPMP
|
FN
|
CETF&CI
|
1PalM;2D;2c;3Oct;4P
- Respiratory stimulation by simultaneous action of the F-pathway and N-pathway with convergent electron flow in the FN-pathway for evaluation of an additive or inhibitory effect of F.
- OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
|
5G
|
PalOctPGMP
|
FN
|
CETF&CI
|
1PalM;2D;2c;3Oct;4P;5G
- Respiratory stimulation by simultaneous action of the F-pathway and N-pathway with convergent electron flow in the FN-pathway for evaluation of an additive or inhibitory effect of F.
- OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
|
6U
|
PalOctPGME
|
FN
|
CETF&CI&II
|
1PalM;2D;2c;3Oct;4P;5G;6U
|
7S
|
PalOctPGMSE
|
FNS
|
CI&CII
|
1PalM;2D;2c;3Oct;4P;5G;6U;7S
- Respiratory stimulation by simultaneous action of the F-pathway, N-pathway, and S-pathway, with convergent electron flow in the FNS-pathway for reconstitution of TCA cycle function and additive or inhibitory effect of F.
- Noncoupled electron transfer state, ET state, with ET capacity E.
|
8Rot
|
SE
|
S
|
CII
|
1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot
|
9Ama
|
ROX
|
|
|
1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot;9Ama
- Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).
|
Step
|
Respiratory state
|
Pathway control
|
ET-Complex
|
Comment
|
## AsTm
|
AsTmE
|
CIV
|
CIV
|
|
## Azd
|
CHB
|
|
|
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Β» Coupling control state
- Β» ET capacity
- Β» OXPHOS capacity
- Β» LEAK respiration
- Pathway control
- Β» Electron transfer pathway
- Β» Fatty acid oxidation pathway control state, F
- Β» NADH electron transfer-pathway state, N
- Β» Succinate pathway control state, S
- Β» NS-pathway control state, NS
- Β» Glycerophosphate pathway control state, Gp
- Β» Complex IV single step, CIV
- Β» Anaplerotic pathway control state
- Main fuel substrates
- Β» Glutamate, G
- Β» Glycerophosphate, Gp
- Β» Malate, M
- Β» Octanoylcarnitine, Oct
- Β» Pyruvate, P
- Β» Succinate, S
- Glossary
- Β» List of SUIT states
- Β» SUIT concept
Strengths and limitations
Compare SUIT protocols
1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot9Ama;10Tm;11Azd
MitoPedia concepts:
MiP concept,
SUIT protocol,
Recommended
MitoPedia methods:
Respirometry
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