SUIT-022 O2 ce D051: Difference between revisions
From Bioblast
(Created page with "{{MitoPedia |abbr=? |description=350px |info='''A''' Determination of the respiration due to the alternative oxidase pathway -'''SUIT-022''...") ย |
No edit summary |
||
Line 7: | Line 7: | ||
}} | }} | ||
::: '''[[MitoPedia: SUIT]]''' | ::: '''[[MitoPedia: SUIT]]''' | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ce | ||
::: '''[[SUIT protocol pattern]]:''' | ::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM | ||
The SUIT-022 O2 ce D051 has been designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant | The SUIT-022 O2 ce D051 has been designed to provide an estimation of the mitochondrial respiration due to the [[alternative oxidase]] pathway, which is [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant for the specific case of [[microalgal]] cells. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the cytochrome ''c'' pathway (see [[SUIT-023]]). | ||
__TOC__ | __TOC__ | ||
ย Communicated by | ย Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-04-09) | ||
{{Template:SUIT-022 O2 ce D00#}} | {{Template:SUIT-022 O2 ce D00#}} | ||
== Strengths and limitations == | == Strengths and limitations == | ||
:::* SUIT-022 O2 ce D051 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] | :::* SUIT-022 O2 ce D051 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of the [[alternative oxidase]] on [[microalgal]] cells. | ||
::: | :::* The concentrations of inhibitors used in this protocol have been optimised for the specific case of the microalgal model species ''Chlamydomonas reinhardtii'' | ||
:::+ Short duration of experiment | :::+ Short duration of experiment | ||
Line 28: | Line 28: | ||
== Compare SUIT protocols == | == Compare SUIT protocols == | ||
::::* [[SUIT-023 O2 ce D053]] has to be used in parallel to obtain complete information of the alternative oxidase | ::::* [[SUIT-023 O2 ce D053]] has to be used in parallel to obtain complete information of the contribution of both [[alternative oxidase]] and cytochrome ''c'' oxidase pathways to mitochondrial respiration. | ||
Revision as of 11:32, 9 April 2019
Description
Abbreviation: ?
Reference: A Determination of the respiration due to the alternative oxidase pathway -SUIT-022
SUIT number: D051_ce1;ce2KCN;ce3SHAM
O2k-Application: O2
- MitoPedia: SUIT
- SUIT-category: ce
- SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM
The SUIT-022 O2 ce D051 has been designed to provide an estimation of the mitochondrial respiration due to the alternative oxidase pathway, which is cyanide-and antimycin A-resistant for the specific case of microalgal cells. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the cytochrome c pathway (see SUIT-023).
Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-09)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2KCN | ROX or ROUTINE | ce1;ce2KCN
| ||
ce3SHAM | ROX | ce1;ce2KCN;ce3SHAM
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- SUIT-022 O2 ce D051 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on microalgal cells.
- The concentrations of inhibitors used in this protocol have been optimised for the specific case of the microalgal model species Chlamydomonas reinhardtii
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
- SUIT-023 O2 ce D053 has to be used in parallel to obtain complete information of the contribution of both alternative oxidase and cytochrome c oxidase pathways to mitochondrial respiration.
References
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry