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...ic normalization for the volume of the instrumental chamber (the measuring system). Metabolic ''flow'', ''I'', per [[Count |countable]] object increases as t

...can be stimulated with 600 μM PFOA after inhibition of the phosphorylation system.

|tissues=Nervous system

...[[O2k-Workshop]]. Alternatively, oxygen flux can be measured in an [[open system]] mode of operation, using the [[Titration-Injection microPump]] (TIP2k) fo ...Finally, the basic O2k-Workshop provides an overview on [[O2k-MultiSensor System]] applications, for the simultaneous measurement of respiration and [[mitoc

...e have had no real issues in almost four years. I started with the general system which requires water baths etc and really it was baptism of fire, and when

...respiratory complexes of the electron transfer-pathway and phosphorylation system. Novel developments are presented on substrate-uncoupler-inhibitor titratio

|description=The component of the electron transfer system located in the mitochondrial matrix ('''matrix-ETS''') is distringuished fr

...MIT2017 IOC121.JPG| The [[Oroboros O2k]] with the [[ISS-Integrated Suction System]], [[O2k-Titration Set]] and [[OroboPOS-Service Kit]]

...ndida auris'' recombinant AOX expressed in a self-assembled proteoliposome system. Sci Rep 11:14748. ...V_max) with respect to ubiquinol-10 have been determined. The PL system has also been employed in dose-response assays with novel AOX inhibitors. S

...ex CI (CI), nicotinamide nucleotide transhydrogenase (NNT) and antioxidant system significantly decreased the ability of mitochondria to exhibit mTRS. NADH l

...eral subunits which are involved in energy transduction of the respiratory system. [[Respiratory Complexes#Respiratory Complexes - more than five |» '''MiPN ...electron transfer pathway (CI to CIV, CGpDH and CETF) and phosphorylation system (CV). From [[Gnaiger 2020 BEC MitoPathways]].]]

...gest that by regulating calcium uptake the mitochondrial electron transfer system is a hub for coordinating cellular adaption under functional hypoxia.<br> |tissues=Heart, Skeletal muscle, Nervous system, Other cell lines

...e S are identical to the volume ''V'' and mass ''m'' of the experimental [[system]]. A pure sample S may be mixed with other components to be investigated as ...information on the system, often to serve as a basis for evaluation of the system (diagnosis) or for a decision on intervention (therapy, production process)

...nce material that is used to assess the bias of measurement of a measuring system.

...an people. In one of CAAD’s core facilities, the Oroboros® O2k-MultiSensor system will be combined with the Seahorse XF24 and the Magnettech MS400 EPR spectr |tissues=Nervous system

|keywords=Adenine triphosphate, Electron transport system, Mitochondrial membrane potential, Oxidative stress, Peripheral blood monon

|tissues=Nervous system

...gest that by regulating calcium uptake the mitochondrial electron transfer system is a hub for coordinating cellular adaption under functional hypoxia.

...pecifically inhibited Complex I linked respiration and the phosphorylation system, such that meaningful applications had to be restricted to Complex II linke

...management systems - requirements''': The adoption of a quality management system is a strategic decision for an organization that can help to improve its ov :::: — Plan: establish the objectives of the system and its processes, and the resources needed to deliver results in accordanc

...([[Complex IV|Complex IV, CIV]]), '''ascorbate''' is added as regenerating system to maintain [[TMPD]] in a reduced state. It has to be titrated into the re

<span style="font-size:105%; color:#424242">'''Are you registered with System for Award Management (SAM)?'''</span>

...Quote'': Bureau International des Poids et Mesures 2019 The International System of Units (SI), p. 127)}. ...': "expression of the dependence of a quantity on the base quantities of a system of quantities as a product of powers of factors corresponding to the base q

...laboratories, although they may be part of a larger laboratory network or system. ...ary sample]]''': specimen; set of one or more parts initially taken from a system. In some countries, the term “specimen” is used instead of primary samp

...sic''' is customized particularly for users interested in [[O2k-Catalogue: O2k-MultiSensor|MultiSensor]] applications, particularly ion selective electrodes for measu |Oxidative phosphorylation system.

...17-216. [[Bureau International des Poids et Mesures 2019 The International System of Units (SI) |ISBN 978-92-822-2272-0]]</ref>. IUPAC guidelines are followe ...horguidelines BEC guidelines], 'manuscripts must adhere to [[International System of Units |SI units]] and IUPAC recommendations. [[MitoEAGLE]] recommendatio

...ontributes to the total change of concentration, d''c''_''j'' (a system variable or variable of state). In open systems at steady-state, d<sub>r</s ...= -1. When oxygen consumption is measured in aqueous solution in a closed system, the volume-specific [[oxygen flux]], ''J''_''V'',O₂,

...plots should be selected.(Oxygen O2, Amperometric Amp, Potentiometric pX, System channels) ===Tab: System channels===

...pacity to a larger extent in heart than liver. The ADP-ATP phosphorylation system exerted a higher control over OXPHOS (CI+II) in heart than liver, making tr ...r comparative mitochondrial physiology and pathology, and for a variety of O2k-MultiSensor protocols applied for functional diagnosis of mitochondrial performance. Th

...nergetic parameters, including oxygen consumption rates, electron transfer system (ETS)-related coenzyme Q (mtCoQ) redox state and production of reactive oxy |tissues=Nervous system, Liver

...pecifically inhibited Complex I linked respiration and the phosphorylation system, such that meaningful applications had to be restricted to Complex II linke

...he city centre, but is also well connected to Ljubljana’s public transport system – Ljubljanski potniški promet (LPP)

...spiration. Mitochondrial capacity was not limited by the electron transfer system, although the presence of multiple substrates potentially flooded the Q-jun

...nd [[CIII]]) at their quinone-binding sites and with the [[Three-electrode system |detecting electrode]].

...to the modulation of the glutamatergic system and maintenance of the redox system. Thus, considering the few studies focused on the direct effects of GUO on

...l, kit, specimen receptacle, software, instrument, apparatus, equipment or system, whether used alone or in combination with other diagnostic goods for in vi

...ying oxygen concentrations. This method can be used for any ROS-generating system where the effect of oxygen level on H₂O₂ production n |tissues=Nervous system

...treated with nerve growth factor. This makes PC12 cells useful as a model system for neuronal differentiation.

...ma cells. We assessed the potential of DOX-Tf conjugate as a drug delivery system, monitoring its cytotoxicity using the MTT assay and ATP measurements. More

...degradation. The effect of inhibitors of the chloroplast electron transfer system on photodegradation remains to be investigated.

...r session-specific value (Lab-default or Session value) different from the System default.

...hibited higher sensitivity to changes in mtMP than TMRM. This multi-sensor system measured mitochondrial parameters in the brain of transgenic mice that mode |tissues=Nervous system

...ities downstream of Q to O₂, and (''3'') the load on the OXPHOS system utilizing or dissipating the protonmotive force. |keywords=coenzyme Q CoQ, Q-junction, Q-redox state, electron transfer system ETS, ETS-reactive Q-pool Q, mitochondrial coenzyme Q mtCoQ, supercomplexed

...ies of oxidative phosphorylation ([[OXPHOS]]) and of the electron transfer system ([[ETS]]), and (iii) monitor simultaneously oxygen consumption (''J''<sub>O |tissues=Nervous system

...hrough several mt-Complexes (CI, CII, CETF, etc) reduces electron transfer system (ETS)-reactive ubiquinone (UQ) to ubiquinol (UQH₂), which is oxi # Gnaiger E (2023) Complex II ambiguities ― FADH2 in the electron transfer system. https://doi.org/10.26124/mitofit:2023-0003.v4

...surements. If I proceed with the perception that DatLab is part of the O2k-system, then the part of the O2k-Manual that instructs me to add the cells into th ...are O2k-Chamber in box A(1K). This is followed by popping up 2K to the O2k-System '''S'''. In my '''TC'''-mode there is a '''T'''heoretical voice that says:

...2k-MultiSensor System with ion selective electrodes (ISE) '''[[MiPNet15.03 O2k-MultiSensor-ISE |MiPNet15.03]]'''

...up> fluxes, and the opening of mPTP in various biological samples. In this system the duration of stimulated Ca²⁺ fluxes may provide a novel param

...as an open system with oxygen transport between the aqueous phase and the system boundary (2). For measurement of NO in experimental chambers, however, the

...ypoxia can lead to latent phenotypical changes in the adult cardiovascular system, including altered cardiac structure/function and increased susceptibility

...ygen utilization, maximal oxidative phosphorylation and electron transport system capacities in the skeletal muscle.

...nts on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed. MitoFit Preprint Arch 2020.4 [https://www.mitofit.org

...f respiration is due to the high affinity of the oxidative phosphorylation system for ADP."

...he (negative) time derivative of oxygen concentration measured in a closed system, volume-specific [[proton flux]] is the time derivative of proton concentra ...imultaneously with the oxygen signals in both O2k-chambers. A new pH probe system is described here, consisting of separate reference and measuring electrod

...rimental design by combining the technical advantages of closed and [[open system]]s approaches. ...luxes. Oxygen concentrations may decline below a lower limit in the closed system, in which case a reoxygenation must be performed by intermittently opening

...ibroblasts and heart fibres, acting on Complex II of the electron transfer system. ...oduction following downstream (of CII) inhibition of the electron transfer system (using antimycin A).”

|tissues=Nervous system

...rain tissue. Astrocytes, a prominent glial cell within the central nervous system (CNS), play a large role in regulating oxidative homeostasis within the dev |tissues=Nervous system

:::: 'The video support section describing several aspects of the O2k system is an incredible resource for users, and I would only suggest to add as man

...osphorylation suggested that the limiting influence of the phosphorylation system is reduced in hybrids. This likely helped offset impairments in electron tr

...ease of MDA levels was prevented by MEL and RSV. Regarding the antioxidant system, sulfite increased glutathione concentrations in hippocampus and striatum. |tissues=Nervous system

...ities downstream of Q to O₂, and (''3'') the load on the OXPHOS system utilizing or dissipating the protonmotive force. |keywords=coenzyme Q; CoQ, Q-junction, Q-redox state, electron transfer system; ETS, ETS-reactive Q-pool; Q, mitochondrial coenzyme Q; mtCoQ, supercomplex

...ssions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with su

...is procedure the O2k-chamber remains closed, decreasing disturbance of the system and allowing for quicker POS stabilization.

...letal muscle]] ••• [[MiP2013 Tissue and cell: Nervous system|Brain;Nervous system]]'''

...orrespondingly low respiratory flux per volume, the oxygen capacity of the system is exhausted slowly, allowing sufficient time to evaluate the stability of

...ties of the ETS downstream of Q to O2 combined with the load on the OXPHOS system utilizing or dissipating the protonmotive force. A three-electrode sensor w

...dria extracted from adult whole mouse heart using the Oroboros Oxygraph-2k system. The method provides detailed procedures for the preparation of mitochondri

...absolutely fantastic experience and strengthened my belief in not only the system, but the entire Oroboros Team as well. I first want to thank all of you fo * '''TPP Basic''' is customized particularly for users interested in [[O2k-MultiSensor| MultiSensor]] applications, particularly ion selective electrodes for meas

...on biological systems should be conducted at the temperature at which the system usually operates, the so-called '''physiological temperature''', which is f

...of cytochrome redox state combined with high-resolution respirometry. The system was tested in an investigation using beef and mouse heart isolated mitochon

...DNA (compared to wild-type), mostly in complex I of the electron transport system, while the other has a single critical SNP difference at the level of compl

...s the sum of NAD(P)⁺ and NAD(P)H — and of the electron transfer system (ETS)-reactive coenzyme Q-pool [1] are linked in the N-pathway but are regu

...ctase (TrxR) can be used to quantified the contribution of the thioredoxin system (Trx) [1].

...lin tolerance test (ipITT), the comprehensive laboratory animal monitoring system (CLAMS) and Oroboros O2k analysis.

|tissues=Nervous system, Liver

|tissues=Nervous system

...ndrial function, redox status, and spatial learning in the central nervous system but has limited impact on peripheral (liver and muscle) metabolism with age |tissues=Skeletal muscle, Nervous system, Fat

|tissues=Nervous system

...are increasing epidemics that significantly impact the peripheral nervous system and lead to negative changes in sensation and peripheral nerve function. Re |tissues=Nervous system

...le mitochondria, equine OXPHOS capacity was limited by the phosphorylation system to 0.85±0.10 (''N'' = 32) of electron transfer capacity, independent of fi

...piration''' oxygen is consumed by a putative respiratory electron transfer system (ETS) within the thylakoid membrane of the [[chloroplasts]] and ATP is prod

...ween expected metabolic supply/demand (as dictated by the circadian timing system and light/dark-cycle) and the actual metabolic supply/demand (as dictated b

...are increasing epidemics that significantly impact the peripheral nervous system and lead to negative changes in sensation and peripheral nerve function. Re |tissues=Nervous system

...ry substrates, without considering the plurality of the electron transport system. In this study, mitochondrial function was analyzed under conditions of iso |tissues=Nervous system

...ce, as studied by application of protocols, in which the [[phosphorylation system]] is either inhibited (lack of ADP; inhibition by oligomycin), activated (s

...ty to NADH. Maximal flux rates and the capacity for the electron transport system to uncouple were also altered at 25°C. These data indicate that mitochondr

...en peroxide production by Nox in isolated synaptosomes, the HRP/Amplex Red system was associated with greater oxygen consumption and higher rates of hydrogen |tissues=Nervous system

|tissues=Nervous system

...ssions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with su

...il 2012), which can be applied as an extension of the present O2k-Core HRR System (Figure 1). In the second phase, the experience gained with the modular app ...ting a light source (LED) and a photodiode with aFigure 4: O2k-MultiSensor System with TPP electrodes inserted through the stoppers into the two chambers; th

|tissues=Heart, Nervous system

...ins unclear. Under low energy demand, the mitochondrial electron transport system (ETS) is particularly sensitive to added energy supply (i.e., reductive str |keywords=Beta-oxidation, Bioenergetics, Electron transport system (ETS), Energy metabolism, Hydrogen sulfide, Mitochondrial metabolism, Nicot

|tissues=Nervous system

...em ETS of the mitochondrial inner membrane mtIM) but on the posphorylation system including the F₁F_O-ATPase. ...This could either be with using permeabilized fibers or tissues in the O2k system or developing genetically modified cell lines that allow for the interrogat

...ssions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with su

...ncluding the development of new technology such as using a three-electrode system [1,2] inserted into the Oroboros Q2k to evaluate the redox state of the Coe

...nction is a key point of convergent electron flow in the electron transfer system from mt-dehydrogenases via Complex I (CI), from succinate (S) via Complex I ...ub>/Q_{total reduced=} Qr/Qt) was detected using a three-electrode system [2,3] inserted into the Oroboros Q2k. Experiments were carried out on mitoc

...e presence or absence of site-specific inhibitors of the electron transfer system showed that NAD+ regenerated by Complex I is reduced by the 2-oxoglutarate

::::* 2016-01-27 [[MitoFit Quality Control System]]