Difference between revisions of "Template:SUIT-006 O2 mt D022"
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[[Image:SUIT-MitoFit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | [[Image:SUIT-MitoFit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | ||
== Steps and respiratory states == | == Steps and respiratory states == | ||
[[File: | [[File:1SRot;2D;2c;3U;4Ama.png|400px]] | ||
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Revision as of 16:59, 21 January 2019
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1SRot | SL | S | CII | L, LEAK respiration
Succinate, S ( type S-pathway to Q). Succinate pathway control state (S-pathway) after inhibiting CI with rotenone, which also inhibits the F-pathway. Template:SUIT L n |
2D | SP | S | CII | 1SRot;2D
Succinate, S ( type S-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
3U | SE | S | CII | 1SRot;2D;3U
Succinate, S ( type S-pathway to Q). Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity E (noncoupled ET-state). Test for limitation of OXPHOS capacity P by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET capacity E in mt-preparations: E-P control efficiency and E-L coupling efficiency. In living cells: E-R control efficiency and E-L coupling efficiency. |
4Ama | ROX | Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt). |
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- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary