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Difference between revisions of "Template:SUIT-009 AmR pce D019"

From Bioblast
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| [[S]]
| [[S]]
| CII
| CII
| S<sub>''L''</sub> or S_L: Succinate, S-LEAK respiration, S<sub>''L''</sub>
| 1S
*{{Template:SUIT S}}  
*{{Template:SUIT S}}  
*{{Template:SUIT L(n)}}
*{{Template:SUIT L(n)}}
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|-
|-
| 6Ama
| 5Ama
| [[ROX]]
| [[ROX]]
|  
|  

Revision as of 12:42, 21 February 2019

MitoPedia: SUIT

Steps and respiratory states

Ce1;1Dig;1S;2D;3P;4Rot;5Ama.png

Step State Pathway Q-junction Comment - Events (E) and Marks (M)
0DTPA
  • DTPA is an iron chelator, which decreases the chemical fluorescence background created by the Amplex UltraRed assay. Administration of DTPA into the O2k-chamber is recommended before all other chemicals because the iron chelation capacity of the compound is time-dependent (approx. 10-15 min). However, the experiments can be carried out in the absence of DTPA.
0SOD
  • SOD or superoxide dismutase converts the anion superoxide released by the mitochondria into H2O2, making it accessible to the Amplex UltraRed assay.
0HRP
  • HRP or horseradish peroxidase catalyses the conversion of Amplex UltraRed and H2O2 towards the fluorescent resorufin.
0AmR
Step State Pathway Q-junction Comment - Events (E) and Marks (M)
ce1 ROUTINE ce1
  • ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
1Dig Dig ce1;1Dig
  • Optimum effective digitonin concentration for complete plasma membrane permeabilization.
Step State Pathway Q-junction Comment - Events (E) and Marks (M)
1S SL S CII 1S
2D SP S CII 1S;2D
3P NSP NS CI&II 1S;2D;3P
4Rot SP S CII 1S;2D;3P;4Rot


5Ama ROX
  • Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).



Questions.jpg


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