Difference between revisions of "Template:SUIT text D004"
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The SUIT-001 O2 ce-pce D004 protocol is the reference protocol 1 (RP1) specifically for [[PBMC]]s and [[platelet|PLTs]]. The main difference between the RP1 for PBMCs and PLTs and RP1 for other [[Mitochondrial preparations| mitochondrial preparations]] ([[SUIT-001| see SUIT-001]]) is the absence of [[Octanoylcarnitine|Oct]] in the case of blood cells. | The SUIT-001 O2 ce-pce D004 protocol is the reference protocol 1 (RP1) specifically for [[PBMC]]s (Peripheral Blood Mononuclear Cells) and [[platelet|PLTs]] (platelets). The main difference between the RP1 for PBMCs and PLTs and RP1 for other [[Mitochondrial preparations| mitochondrial preparations]] ([[SUIT-001|see SUIT-001]]) is the absence of [[Octanoylcarnitine|Oct]] in the case of blood cells. | ||
The SUIT-001 O2 ce-pce D004 protocol in combination with [[SUIT-002 O2 ce-pce D007a]] is specially designed to provide a common reference for comparison of respiratory control of [[PBMC|PBMC]] and [[Platelet|platelets]] cells (non-permeabilized cells must be added to the chambers and then permeabilized as a part of the protocol) in a wide variety of species. SUIT-001 O2 ce-pce D004 gives information of [[ROUTINE]] respiration (ce: non-permeabilized cells) and after the plasma membrane permeabilization (pce: permeabilized cells) of the linear coupling control ([[LEAK respiration|''L'']]-[[Oxidative phosphorylation| ''P'']]-[[ET | The SUIT-001 O2 ce-pce D004 protocol in combination with [[SUIT-002 O2 ce-pce D007a]] is specially designed to provide a common reference for comparison of respiratory control of [[PBMC|PBMC]] and [[Platelet|platelets]] cells (non-permeabilized cells must be added to the chambers and then permeabilized as a part of the protocol) in a wide variety of species. SUIT-001 O2 ce-pce D004 gives information of [[ROUTINE]] respiration (ce: non-permeabilized cells) and after the plasma membrane permeabilization (pce: permeabilized cells) of the linear coupling control ([[LEAK respiration|''L'']]-[[Oxidative phosphorylation| ''P'']]-[[ET capacity| ''E'']]) with NADH linked-substrates ([[PM-pathway control state|PM]]). Moreover, the pathway control in ET state ([[NADH_Electron_transfer-pathway_state|N]], [[NS-pathway control state| NS]], [[Succinate pathway control state| S]] and [[SGp-pathway control state| SGp]] pathways) can be evaluated by using this SUIT protocol. SUIT-001 O2 ce-pce D004 can be extended with the CIV assay module. | ||
Latest revision as of 18:33, 5 March 2024
The SUIT-001 O2 ce-pce D004 protocol is the reference protocol 1 (RP1) specifically for PBMCs (Peripheral Blood Mononuclear Cells) and PLTs (platelets). The main difference between the RP1 for PBMCs and PLTs and RP1 for other mitochondrial preparations (see SUIT-001) is the absence of Oct in the case of blood cells. The SUIT-001 O2 ce-pce D004 protocol in combination with SUIT-002 O2 ce-pce D007a is specially designed to provide a common reference for comparison of respiratory control of PBMC and platelets cells (non-permeabilized cells must be added to the chambers and then permeabilized as a part of the protocol) in a wide variety of species. SUIT-001 O2 ce-pce D004 gives information of ROUTINE respiration (ce: non-permeabilized cells) and after the plasma membrane permeabilization (pce: permeabilized cells) of the linear coupling control (L- P- E) with NADH linked-substrates (PM). Moreover, the pathway control in ET state (N, NS, S and SGp pathways) can be evaluated by using this SUIT protocol. SUIT-001 O2 ce-pce D004 can be extended with the CIV assay module.
