Template talk:Next oroboros ecosystem agenda
From Bioblast
Bioblast alert 2020
Next
- Parkinson’s Disease Awareness Month: Dominant mutation in Parkinson’s Disease causes loss of protein function in mitochondria
- - from the O2k-Network »FR Angers Gueguen N« and »BE Leuven Spinazzi M«
- Cornelissen T, Spinazzi M, Martin S, Imberechts D, Vangheluwe P, Bird M, De Strooper B, Vandenberghe W (2020) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2. Hum Mol Genet [Epub ahead of print]. »Bioblast link«
- Parkinson’s Disease Awareness Month: Dominant mutation in Parkinson’s Disease causes loss of protein function in mitochondria
- - from the O2k-Network »DE Nuthetal Klaus S«
- Ost M, Igual Gil C, Coleman V, Keipert S, Efstathiou S, Vidic V, Weyers M, Klaus S (2020) Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress. EMBO Rep [Epub ahead of print]. - »Bioblast link«
- An amazing number of new O2k-Publications from China » O2k-Network Labs China «
- Let's talk COVID-19 - a new culture of communication in science. - Kay Kupferschmidt in Science
- The MitoFit Preprint Archives was created as an Open Access preprint server for mitochondrial physiology and bioenergetics - »MitoFit Preprint Archives«
- Zika virus infection leads to mitochondrial dysfunction
- - from the O2k-Network »BR Rio de Janeiro Galina A«
- Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK (2020) Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes. Sci Rep 10:1218. - »Bioblast link«
- Zika virus infection leads to mitochondrial dysfunction
- - from the O2k-Network »KR Busan Han J« »KR Incheon Kwak HB«
- No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - »Bioblast link«
On hold
- Did you ever think that units for body mass could be an issue? » Body mass «
- Why do we fight gas bubbles? - Dive deep into Oxygen solubility
MitoPedia alert
O2k-Publications alert
Next
O2k-Open Support alert
Next
- While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.
Already added to Agenda - Next and to be added to O2k-Open support alert
- May-04 Mo
- Do you load the correct instrumental background correction accordingly to your range of experimental O2 levels?
- See how to perform the instrumental background test. - » O2 background correction in DatLab« and » Instrumental oxygen background«
- Do you load the correct instrumental background correction accordingly to your range of experimental O2 levels?
- May-11 Mo
- Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.
- Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis - »Steady state«
- Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.
- May-18 Mo
- Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?
- See how to clean and dry the electrical connections of the OroboPOS-Connector.-»Cleaning the electrical connections«
- Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?
- May-25 Mo
- Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?
- Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.-»Switch components approach«
- Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?
Removed for correction
- Do you perform the leaky chamber test after chamber assembly?
- - An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly. - »Leaky chamber test«
- Do you perform the leaky chamber test after chamber assembly?
O2k-Open Support alert circulars
O2k-Open Support alert circular 2020(01): in prep
- New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
- See how you can check that the two blue OroboPOS-Holders are intact. - »O2k-OroboPOS-Holder«
- New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
- New Calibration parameters are transferred to experimental DatLab files
- Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - » Oxygen calibration - DatLab«
- Do you perform O2k-Chamber volume calibration?
- The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - »O2k-Chamber MitoPedia«
- Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
- New Calibration parameters are transferred to experimental DatLab files
- The O2 solubility factor varies according to the medium used and influences the air calibration. - »Oxygen solubility factor«== In preparation ==
For discussion
Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020
- Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions:
- Proven -80 °C solid stability for more than 3 years (3 years 7 months)
- Proven -20 °C solid stability for 1 year
- Proven DMSO stability at room temperature for 1 week
- Proven DMSO stability at -20 °C for 6 months (ongoing)
- Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions:
- As part of the H2020 SME NextGen-O2k project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O2 consumption and production: »PhotoBiology«
- Do you perform a stirrer test during oxygen calibration?
- In DatLab 7.4, instrumental DL-Protocols guide you automatically towards performing the stirrer test in the process of air calibration. It shows immediately, if your OroboPOS has a satisfactory response time. Further details in MitoPedia: »Oxygen calibration - DatLab
- Do you perform a stirrer test during oxygen calibration?
- Is the noise of the ‘O2 slope neg’ acceptable?
- If you are not certain that the noise of the ‘O2 slope neg’ is within a suitable range or you want to confirm that your experimental data will not be affected, see further details: MiPNet06.03 POS-calibration-SOP
- Is the noise of the ‘O2 slope neg’ acceptable?
2021
- 2021-01-06 https://www.jstor.org/stable/1706346?seq=1