WG1 MitoEAGLE protocols, terminology, documentation

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Short-Term Scientific Missions

Management Committee

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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping

WG1 MitoEAGLE protocols, terminology, documentation

WG1 Action

Standard operating procedures and user requirement document: Protocols, terminology, documentation

» WG1 Project application

Many analyses lack statistical power within a single study. Statistical combination of different studies is often difficult due to lack of methodological standardization and harmonization.

Participate

• • • • WG Leader

Kathrin Renner-Sattler, Mag. Dr. - [email protected]

• • • • WG Co-Leader

Marko Vendelin, Dr. - [email protected]

• • • • WG participants: » Talk:WG1 MITOEAGLE protocols, terminology, documentation

• • • • Task group leaders: suggestions discussed at the MitoFit Science Camp 2016 Kuehtai AT, and updated according to the MITOEAGLE MC Meeting 2016 Brussels BE.

• • • • Task group participants: please send us an Email to join: [email protected]

Track record

» 2017-07 MiPschool Obergurgl 2017

» 2017-03 MITOEAGLE 2017 Barcelona ES

» 2016-11 MITOEAGLE 2016 Verona IT

SUIT protocols and terminology

• • • • Task group 1.1 Coupling states and coupling control ratios: consensus statements

TG leaders: Renner-Sattler Kathrin DE / Gnaiger Erich AT / Hoppel Charles L US

TG participants:

• • • • Task group 1.2 SUIT protocols: nomenclature consistent with concept of database

TG leader: Doerrier Velasco Carolina A AT, Gnaiger Erich AT

TG participants: Attimonelli Marcella IT, Krako Nina RS, Vendelin Marko EE

• • • • Task group 1.3 Experimental details in SUIT

TG leaders: Chicco Adam J US/ Hickey Anthony J NZ/ Doerrier Velasco Carolina A AT

TG participants: Casado Pinna Marta ES, Endlicher Rene CZ, Krako Nina RS, Kucera Otto CZ, Sobotka Ondrej CZ, Stankova Pavla CZ

» MitoPedia: Sample preparations

Action

» Pre-publication: Mitochondrial respiratory control states

» MitoPedia: Respiratory control ratios

» MitoPedia: SUIT

» MitoPedia: Media for respirometry

» MitoPedia: Substrates and metabolites

» MiPNet21.06 SUIT reference protocol

» Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of oxidative phosphorylation by temperature in the heart. bioRxiv doi10.1101/103457. - »Bioblast link«

Instrumental platforms: comparisons

• • • • Task group 1.4 Instrumental platforms: comparisons

TG leaders: Molina Anthony JA US

TG participants: Canto Carles CH (suggested), Genova Maria Luisa IT, Tronstad Karl Johan NO (suggested), Vendelin Marko EE, Zorzano Antonio ES (suggested)

Knowledge management system and proficiency test

• • • • Task group 1.5 Knowledge management system

TG leaders: Drinnan Michael UK

TG participants: Vendelin Marko EE, Garcia-Roves Pablo ES

• Towards and beyond a mitochondrial physiology database: filter and amplify more signals from this data, making them more meaningful and valuable for research and medicine.

• • • • Task group 1.6 Proficiency test

TG leaders: Gnaiger Erich AT/ Doerrier Velasco Carolina A AT

TG participants: Genova Maria Luisa IT

Action

» MitoFit-KMP

» MitoFit Quality Control System

» MiPMap

» MitoFit proficiency test

» MiPNet21.07 MitoFit DatLab PT

Documentation

• • • • Task group 1.7 Documentation

TG leaders: Arnould Thierry BE / Votion Dominique-Marie BE

TG participants: Gnaiger Erich AT

Action

» Preprints for Gentle Science

Preprints for MITOEAGLE

1. Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of oxidative phosphorylation by temperature in the heart. bioRxiv doi10.1101/103457. - »Bioblast link«

Data availability

1. Data availability statements in PLOS journals

Dissemination and training

• • • • Task group 1.8 Translation to community in general

TG leader: Makrecka-Kuka Marina LV (Dissemination Coordinator), Rattan Suresh DK

TG participants: Krako Nina RS, Irving Brian A US

• • • • Task group 1.9 Dissemination Scientific community

TG leaders: Makrecka-Kuka Marina LV / Gnaiger Erich AT

TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Laner Verena AT, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos PT, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE

• • • • Task group 1.10 Training, Short Term Scientific Missions

TG leader: Labieniec-Watala Magdalena PL, Klaus Susanne DE

TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos PT, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE

Action

» Media:Dissemination plan MITOEAGLE.docx

» Short-Term Scientific Missions MITOEAGLE

References

» ISO/IEC 17043:2010 General requirements for proficiency testing

» ISO 13528:2015 Statistical methods for use in proficiency testing by interlaboratory comparison

» ISO 15189:2012 Medical laboratories — Particular requirements for quality and competence

» ISO 17511:2003 In vitro diagnostic medical devices

» ISO 9001:2015 Quality management systems - requirements

» ISO 10012:2003 Measurement management systems

General requirements for the competence of testing and calibration laboratories » ISO/IEC 17025:2005