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Cardoso 2021 BEC MgG

From Bioblast


Bioenergetics Communications        
Gnaiger 2020 BEC MitoPathways
       
Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1.
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Bioenergetics Communications
Publications in the MiPMap
Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. https://doi.org/10.26124/bec:2021-0001

» Bioenerg Commun 2021.1. BEC pdf published online 2021-06-30

Cardoso Luiza HD, Doerrier Carolina, Gnaiger Erich (2021) Bioenerg Commun

Abstract: BEC.png doi:10.26124/bec:2021-0001

MgG BEC 2021.1.jpg

For the advanced study of mitochondrial function, high-resolution respirometry is extended by fluorometric measurement of ATP production using the fluorophore Magnesium Green™ (MgG). A common problem with several fluorescent dyes is the inhibition of mitochondrial respiration. In the present study, a coupling control protocol was applied in combination with MgG to measure ATP production simultaneously with respiration for calculation of P»/O2 ratios. MgG at 1.1 µM did not affect respiration through the NADH-linked and succinate-linked pathways. Respiration was not inhibited in any of the coupling control states, hence coupling control efficiencies were not affected by MgG.

Keywords: ATP, ATP production, high-resolution respirometry, Magnesium Green, mitochondria, oxidative phosphorylation, fluorometry, FluoRespirometry Bioblast editor: Gnaiger E O2k-Network Lab: AT Innsbruck Oroboros

ORCID: ORCID.png Cardoso Luiza HD, ORCID.png Doerrier Carolina, ORCID.png Gnaiger Erich

Data availability

Original files are available Open Access at Zenodo repository: 10.5281/zenodo.4916141

Support

Template NextGen-O2k.jpg
Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 859770. An initiative of the MitoEAGLE Task Group of the Mitochondrial Physiology Society.

Keywords


Questions.jpg


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Bioblast links: ATP production - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>



Phosphorylation pathway

» Phosphorylation pathway
» ATP synthase
» Adenine nucleotide translocase
» Phosphate carrier
Phosphorylation pathway substrates
» ADP
» ATP
» Inorganic phosphate
Phosphorylation pathway inhibitors
» Oligomycin
» Carboxyatractyloside
» Atractyloside
» Bongkrekik acid

Coupling control

» Oxidative phosphorylation
» OXPHOS capacity
» P-L control efficiency
» ROUTINE respiration
» R-L control efficiency

Respiratory complexes and coupling

» Complex I
» Complex III
» Complex IV
» Proton pump
» Electron-transfer-pathway state

ATP production measurement

» Magnesium Green


References (Open Access versus Paywall)

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Cited by

Gnaiger 2021 Bioenerg Commun


Gnaiger E (2021) Beyond counting papers – a mission and vision for scientific publication. Bioenerg Commun 2021.5. https://doi:10.26124/BEC:2021-0005

Preprint

Cardoso 2021 MitoFit MgG


Labels: MiParea: Respiration, Instruments;methods 


Organism: Mouse  Tissue;cell: Heart  Preparation: Isolated mitochondria 

Regulation: ATP production, Coupling efficiency;uncoupling  Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, ROX  HRR: Oxygraph-2k, O2k-Fluorometer, NextGen-O2k, O2k-Protocol 

SUIT-006, MgG, MitoEAGLEPublication, BEC2021.5, MitoFit 2022 NADH, O2k-Demo, ATP