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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE muscle 1
| Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM (2020) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. - Updated: 2020-03-08 |
» MitoEAGLE muscle group (WG 2)
Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM (2020) MitoEAGLE
Abstract: Contents
1. Introduction
2. Methods
3. Workflow
4. Conclusions
- Version beta 01
- Version beta 01
Work in progress
Manuscript table and figure blocks
- Section: Workflow # and Title
- Summary: Aims [1] and recommendations
- Specify study group [2], consider sensitive data
- Methods
- Describe results shown in table and figure, as median and inter-quartile range; provide text with full information
- Discussion
- Conclusions, provide full information
- References
- (1) Define the aim, the question:
- Scientific:
- Diagnostic:
- (1) Define the aim, the question:
- (2)
Data analysis
References
MitoEAGLE skeletal muscle comparative studies: MiR05
| Year | Reference | Organism | Tissue;cell | Diseases | |
|---|---|---|---|---|---|
| Jacques 2020 FASEB J | 2019 | Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J 34:2978-86. | Human | Skeletal muscle | |
| Dollerup 2019 J Physiol | 2019 | Dollerup OL, Chubanava S, Agerholm M, Søndergård SD, Altıntaş A, Møller AB, Høyer KF, Ringgaard S, Stødkilde-Jørgensen H, Lavery GG, Barres R, Larsen S, Prats C, Jessen N, Treebak JT (2019) Nicotinamide riboside does not alter mitochondrial respiration, content or morphology in skeletal muscle from obese and insulin resistant men. J Physiol 598:731-54. | Human | Skeletal muscle | |
| Dohlmann 2018 Physiol Rep | 2018 | Dohlmann TL, Hindsø M, Dela F, Helge JW, Larsen S (2018) High-intensity interval training changes mitochondrial respiratory capacity differently in adipose tissue and skeletal muscle. Physiol Rep 6:e13857. | Human | Skeletal muscle Fat | |
| Trewin 2017 PLOS ONE | 2017 | Trewin AJ, Levinger I, Parker L, Shaw CS, Serpiello FR, Anderson MJ, McConell GK, Hare DL, Stepto NK (2017) Acute exercise alters skeletal muscle mitochondrial respiration and H2O2 emission in response to hyperinsulinemic-euglycemic clamp in middle-aged obese men. PLOS ONE 12:e0188421. | Human | Skeletal muscle | Obesity |
| Granata 2016 FASEB J | 2016 | Granata C, Oliveira RS, Little JP, Renner K, Bishop DJ (2016) Mitochondrial adaptations to high-volume exercise training are rapidly reversed after a reduction in training volume in human skeletal muscle. FASEB J 30:3413-23. https://doi.org/10.1096/fj.201500100R | Human | Skeletal muscle | |
| Christensen 2016 J Appl Physiol (1985) | 2016 | Christensen PM, Jacobs RA, Bonne T, Flück D, Bangsbo J, Lundby C (2016) A short period of high-intensity interval training improves skeletal muscle mitochondrial function and pulmonary oxygen uptake kinetics. J Appl Physiol (1985) 120:1319-27. | Human | Skeletal muscle | |
| Vincent 2015 Front Physiol | 2015 | Vincent G, Lamon S, Gant N, Vincent PJ, MacDonald JR, Markworth JF, Edge JA, Hickey AJ (2015) Changes in mitochondrial function and mitochondria associated protein expression in response to 2-weeks of high intensity interval training. Front Physiol 6:51. | Human | Skeletal muscle | |
| Porter 2015 Am J Physiol Endocrinol Metab | 2015 | Porter C, Hurren NM, Cotter MV, Bhattarai N, Reidy PT, Dillon EL, Durham WJ, Tuvdendorj D, Sheffield-Moore M, Volpi E, Sidossis LS, Rasmussen BB, Børsheim E (2015) Mitochondrial respiratory capacity and coupling control decline with age in human skeletal muscle. Am J Physiol Endocrinol Metab 309:E224-32. | Human | Skeletal muscle | Aging;senescence |
| Robach 2014 Med Sci Sports Exerc | 2014 | Robach P, Bonne T, Flück D, Bürgi S, Toigo M, Jacobs R, Lundby C (2014) Hypoxic training: effect on mitochondrial function and aerobic performance in hypoxia. Med Sci Sports Exerc 46:1936-45. | Human | Skeletal muscle | |
| Jacobs 2013 J Appl Physiol | 2013 | Jacobs R, Flück D, Bonne TC, Bürgi S, Christensen PM, Toigo M, Lundby C (2013) Improvements in exercise performance 1 with high-intensity interval training 2 coincide with an increase in skeletal muscle mitochondrial content and function. J Appl Physiol 115:785-93. | Human | Skeletal muscle | |
| Brands 2011 Metabolism | 2011 | Brands M, Hoeks J, Sauerwein HP, Ackermans MT, Ouwens M, Lammers NM, van der Plas MN, Schrauwen P, Groen AK, Serlie MJ (2011) Short-term increase of plasma free fatty acids does not interfere with intrinsic mitochondrial function in healthy young men. Metabolism 60:1398-405. | Human | Skeletal muscle |
MitoEAGLE skeletal muscle comparative studies: Buffer Z
MitoEAGLE skeletal muscle: Blebbistatin
| Year | Reference | Organism | Tissue;cell | Diseases | |
|---|---|---|---|---|---|
| Doerrier 2019 MitoFit Preprint Arch EA | 2019 | Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM (2019) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. https://doi.org/10.26124/mitofit:ea19.MiPSchool.0009 | Human | Skeletal muscle | |
| Perry 2011 Biochem J | 2011 | Perry CG, Kane DA, Lin CT, Kozy R, Cathey BL, Lark DS, Kane CL, Brophy PM, Gavin TP, Anderson EJ, Neufer PD (2011) Inhibiting myosin-ATPase reveals a dynamic range of mitochondrial respiratory control in skeletal muscle. Biochem J 437:215-22. | Human Rat | Skeletal muscle |
- Picked up
- Picked up
Open questions
Milestones
- In progress (2020)
- March 2020: Figures, figures legends and results by Garcia-Roves PM and Doerrier C. Contribution from Gnaiger E.
- March 2020: Table with skeletal muscle comparative studies performed by Garcia-Roves PM and Larsen S.
- Feb 2020: Oxygen dependence analysis performed by Doerrier C.
- From 2019-01-31 to 2019-02-03: WG 1 and 2 human skeletal muscle workshop and retreat MitoEAGLE_Obergurgl_2019-01-31
- Jan 2019: Data re-analysis performed by Doerrier C, Gama-Perez P, Garcia-Roves PM and Larsen S.
- From 2018-08-06 to 2018-08-10: MitoEAGLE Copenhagen 2018. Preliminary data analysis performed by Doerrier C, Gama-Perez P, Garcia-Roves PM and Pesta D.
- 2017 - Project background: collaboration with Coen P in Orlando.
Dissemination
Publication
- A Working Group of the COST Action MitoEAGLE is preparing a manuscript 'Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers'. The group is working on it with Open Access as a ‘MitoEAGLE preprint’ and the ultimate aim of publication in a scientific journal.
- Compare: Gnaiger 2019 MitoFit Preprints
Coauthors
Publication strategy
- Acknowledgements: We thank M. Beno for management assistance, and Clara Prats for the microscopy. This publication is based upon work from COST Action CA15203 MitoEAGLE, supported by COST (European Cooperation in Science and Technology).
Labels: MiParea: Respiration, Instruments;methods
Organism: Human
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, NS, ROX
HRR: Oxygraph-2k
MitoFitPublication, MitoEAGLEPublication, skeletal muscle
