Gnaiger 2019 MitoFit Preprints
Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. https://doi.org/10.26124/mitofit:190001.v6. — Published: 2020-05-20 Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 |
» MitoFit Preprint Arch 2019.1.v6
Mitochondrial respiratory states and rates
MitoFit Prep 2019.1.v6. (2019) MitoFit Prep
Abstract: Version 6 (v6) 2019-08-30 doi:10.26124/mitofit:190001.v6
- Versions (v5) 2019-07-24; (v4) 2019-05-20; (v3) 2019-04-24; (v2) 2019-03-15; (v1) 2019-02-12 - »Link to all versions«
- Published online: 2020-05-20
- » Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. doi:10.26124/bec:2020-0001.v1. »Bioblast link«
As the knowledge base and importance of mitochondrial physiology to human health expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow guidelines of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols to the nomenclature of classical bioenergetics. We endeavour to provide a balanced view on mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of databases of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery. • Keywords: Mitochondrial respiratory control, coupling control, mitochondrial preparations, protonmotive force, uncoupling, oxidative phosphorylation, OXPHOS, efficiency, electron transfer, ET; proton leak, LEAK, residual oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux, O2 • Bioblast editor: Gnaiger E
Authors: MitoEAGLE Task Group
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- Erich Gnaiger
- Chair COST Action CA15203 MitoEAGLE
- T +43 512 566796 15, F +43 512 566796 20
- [email protected] | www.mitoeagle.org
- Coauthors (listed in alphabetical order); number of coauthors (2019-09-16): 621
- Many coauthors have made significant additions and suggestions for improvement of the manuscript. All coauthors confirm to have read the final manuscript, and to agree to implement the recommendations into future manuscripts, presentations and teaching materials.
- Disclaimer: This article was prepared while Joshua P. Fessel was employed at Vanderbilt University Medical Center. The opinions expressed in this article are the author's own and do not reflect the view of the National Institutes of Health, the Department of Health and Human Services, or the United States government.
- Copyright: © 2019 Gnaiger et al.
- This is an Open Access preprint (not peer-reviewed) distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited. © remains with the authors, who have granted MitoFit an Open Access preprint licence in perpetuity.
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From Version 4 to 5
On World Metrology Day (2019-05-20) the redefinition of the SI units came into force. At this occasion, Version 4 of the MitoEAGLE preprint on 'Mitochondrial respiratory rates and states' was released, in line with our emphasis on SI units for reporting data on mitochondrial respiratory physiology.
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Corrigendum
- Section 2.5.4: Sulfur dioxygenase is related to ET, hence should not be listed here in relation to Rox.
Comments and communication
Linking COST Actions and MiPsociety
The MitoFit preprint
- » Manuscript phases and versions
Manuscript phases and versions - an open-access apporach
- This manuscript on ‘Mitochondrial respiratory states and rates’ is a position statement in the frame of COST Action CA15203 MitoEAGLE. The list of coauthors evolved beyond phase 1 in the bottom-up spirit of COST.
- The global MitoEAGLE network made it possible to collaborate with a large number of coauthors to reach consensus on the present manuscript. Nevertheless, we do not consider scientific progress to be supported by ‘declaration’ statements (other than on ethical or political issues). Our manuscript aims at providing arguments for further debate rather than pushing opinions. We hope to initiate a much broader process of discussion and want to raise the awareness on the importance of a consistent terminology for reporting of scientific data in the field of bioenergetics, mitochondrial physiology and pathology. Quality of research requires quality of communication. Some established researchers in the field may not want to re-consider the use of jargon which has become established despite deficiencies of accuracy and meaning. In the long run, superior standards will become accepted. We hope to contribute to this evolutionary process, with an emphasis on harmonization rather than standardization.
- Phase 1: The protonmotive force and respiratory control
- » The protonmotive force and respiratory control - Discussion
- 2016-11 MitoEAGLE Verona 2016
- 2017-03 MitoEAGLE Barcelona 2017
- 2017-04-09 to 2017-09-18 (44 versions)
- 2017-07 MiPschool Obergurgl 2017
- 2017-09-21 to 2018-02-06 (21 versions)
- » MitoEAGLE preprint 2017-09-21 - Discussion
- 2017-11-11: Print version (16) for MiP2017/MitoEAGLE Hradec Kralove CZ
- Phase 2: Mitochondrial respiratory states and rates: Building blocks of mitochondrial physiology Part 1
- » MitoEAGLE Task Group States and rates - Discussion
- 2018-02-08 (42 versions up to present)
- 2018-08 EBEC2018 Budapest HU - Poster
- 2018-09 MiP2018/MitoEAGLE Jurmala LV
- 2018-10 MiPschool Tromso-Bergen 2018
- Phase 3: 2019-02-12: Publication as a preprint: MitoFit Preprints with DOI number, providing widely accepted visible proof that the publication is citable. - See MitoFit DOI Data Center (2018-12-12 submission).
- Phase 4: Journal submission
- Target: CELL METABOLISM, aiming at indexing by The Web of Science and PubMed.
- Coauthors
- 2017-09-21 Version 01: 105 coauthors
- 2017-10-15 Version 10: 131 coauthors
- 2018-01-18 Version 20: 168 coauthors
- 2018-02-26 Version 30: 225 coauthors
- 2018-08-20 Version 40: 350 coauthors - EBEC Poster
- 2018-10-17 Version 44: 426 coauthors - MiPschool Tromso-Bergen 2018
- 2018-12-12 Version 50: 517 coauthors - Submission to the preprint server bioRxiv not successful
- 2019-02-12 Preprint version 1: 530 coauthors
- 2019-03-15 Preprint version 2: 533 coauthors
- 2019-04-24 Preprint version 3: 533 coauthors
- 2019-05-20 Preprint version 4: 542 coauthors
- 2019-07-24 Preprint version 5: 612 coauthors
- 2019-08-30 Preprint version 6: 622 coauthors - Preprint publication doi:10.26124/mitofit:190001.v6
- Coauthors
- BEC 2020.1. - Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. doi:10.26124/bec:2020-0001.v1. - »Bioblast link«
Cited by
- Gnaiger E (2019) Editorial: A vision on preprints for mitochondrial physiology and bioenergetics. MitoFit Preprint Arch doi:10.26124/mitofit:190002.v2.