MitoPedia alert - 2023
- A collection from the »Bioblast Agenda«
||Protocols that SUIT your research question|
Using Substrate-Uncoupler-Inhibitor-Titration protocols you can study mitochondrial respiratory control through a sequence of coupling and pathway control states induced by multiple titrations within a single experimental assay.
- »SUIT protocols« and »SUITbrowser«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«
|| When should I analyze my data as flux control ratios (FCRs)?|
FCRs enable the comparison of samples normalized with various mitochondrial content parameters or when sample concentration is unknown. This is achieved by internally normalizing O2 fluxes in different respiratory states to the maximum flux in a common reference state.
- »Flux control ratio«
- communicated by »Baglivo Eleonora« and »Timon-Gomez Alba«
|| How to interpret cytochrome c efficiency |
Stimulation of OXPHOS or ET capacity upon addition of cytochrome c provides evidence of cytochrome c loss, indicating damage of the mitochondrial outer membrane, which could be caused by sample preparation or pathological conditions.
- »Cytochrome c control efficiency«
- communicated by »Schmitt Sabine« and »Leo Elettra«
||What is the best fluorophore and concentration thereof for measuring mt-membrane potential?|
It depends on sample characteristics - test the concentration of different fluorophores (safranin, TMRM and rhodamine 123) with your specific model to evaluate for inhibitory or uncoupling effects on respiration.
- »mt-membrane potential measurement«
- communicated by »Grings Mateus« and »Cardoso Luiza«
|| Do you know the difference between uncoupled, noncoupled and dyscoupled respiration? |
Noncoupled respiration is induced experimentally for evaluation of ET capacity, while uncoupling and dyscoupling are respectively caused by
physiological and pathological conditions that exert an influence on proton leak and slip.
- »Noncoupled respiration«, »BEC 2020.01«
- communicated by »Leo Elettra« and »Cardoso Luiza«
|| Are pyruvate, glutamate, and malate substrates of Complex I?|
No, NADH is a Complex I substrate. However, pyruvate, glutamate and malate are used in high-resolution respirometry as substrates for mitochondrial dehydrogenases to generate NADH in the mitochondrial matrix because NADH cannot cross the mitochondrial inner membrane.
- »NADH electron transfer-pathway state« and »Complex I«
- communicated by »Cardoso Luiza« and »Grings Mateus«
||Respiration might be inhibited in the presence of high concentrations of some saccharides due to the Crabtree effect |
It is observed in multiple cell types, such as tumor cells, bacteria or yeast, and it can be measured using SUIT-003 protocols.
- »Crabtree effect«, »SUIT-003«
- communicated by »Timon-Gomez Alba« and »Leo Elettra«
||Here is a system of terms and abbreviations for mitochondrial respiratory pathway and coupling control states.|
Although states and rates are distinguished by different symbols for coupling control states, such a distinction is not suggested for pathway control states and rates to avoid handing and overwhelming number of different symbols.
- »MitoPedia: Pathway and coupling control states«
||Decline of mitochondrial fitness in overweight states is a biomarker of the systemic mitObesity syndrome, providing a mechanistic link between common obesity and associated chronic comorbidities. |
Body mass excess provides an evidence-based index of underweight, overweight and obesity across all heights and ages, applicable to all populations for which the healthy reference baseline is established.
- »Body mass excess and mitObesity«
||What is ROUTINE respiration?|
ROUTINE respiration is the respiratory activity of living (non-permeabilized) cells in the physiological coupling state, which is controlled by cellular energy demand, energy turnover and the degree of coupling to phosphorylation.
- »Gnaiger 2020 BEC MitoPathways - Chapter 2.4«
- communicated by »Schmitt Sabine«
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