O2k-Virtual Support self-study material: Difference between revisions
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== General information == | == General information == | ||
:::: To benefit optimally from the tutored training sessions of the | :::: To benefit optimally from the tutored training sessions of the O2k-Virtual Support, please make yourself familiar with '''Materials for self-study''' in advance. | ||
== O2k-Basic == | == O2k-Basic == | ||
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:::* [[O2k-FluoRespirometer|O2k-Manual: O2k-FluoRespirometer]] | :::* [[O2k-FluoRespirometer|O2k-Manual: O2k-FluoRespirometer]] | ||
:::* [[MiPNet19.18B POS-service|O2k-Manual: POS Service]] | :::* [[MiPNet19.18B POS-service|O2k-Manual: POS Service]] | ||
:::* Step 1: [[Image:YouTube Play Button.svg .png|20px|link | :::* Step 1: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/wMDcSypG3O4]] [https://youtu.be/wMDcSypG3O4 O2k-Videosupport: OroboPOS from Perspex housing] | ||
:::* Step | :::* Step 2: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/HC6MLXO08k8]] [https://youtu.be/HC6MLXO08k8 O2k-Videosupport: Cathode cleaning] | ||
:::* Step 3: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/sDoU69-SLq0]] [https://youtu.be/sDoU69-SLq0 O2k-Videosupport: Anode cleaning] | |||
:::* Step 4: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/iGafaPa4OyA]] [https://youtu.be/iGafaPa4OyA O2k-Videosupport: Membrane mounting] | |||
:::* Step 3: [[Image:YouTube Play Button.svg .png|20px|link | :::* Step 5: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/0EfCReolzdk]] [https://youtu.be/0EfCReolzdk O2k-Videosupport: DL7 Start DatLab7] | ||
:::* Step 4: [[Image:YouTube Play Button.svg .png|20px|link | :::* Step 6: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ O2k-Videosupport: DL7 Insert OroboPOS] | ||
:::* Step 5: [[Image:YouTube Play Button.svg .png|20px|link | :::* Step 7: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/1MSsI1ePMu8]] [https://youtu.be/1MSsI1ePMu8 O2k-Videosupport: Insert O2k Chamber] | ||
:::* Step 6: [[Image:YouTube Play Button.svg .png|20px|link | :::* Step 8: [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/zZI7O5zAruw]] [https://youtu.be/zZI7O5zAruw O2k-Videosupport: O2k Chamber volume calibration] | ||
:::* Step 7: [[Image:YouTube Play Button.svg .png|20px|link | |||
:::* Step 8: [[Image:YouTube Play Button.svg .png|20px|link | |||
=== Part 1.2: DatLab === | === Part 1.2: DatLab and O<sub>2</sub> calibration === | ||
:::* [[MitoPedia: DatLab]] | :::* [[MitoPedia: DatLab]] | ||
:::* [[MiPNet26.06 DatLab 7: Guide|O2k-Manual: DatLab 7 Guide]] | :::* [[MiPNet26.06 DatLab 7: Guide|O2k-Manual: DatLab 7 Guide]] | ||
:::* [[Run DL-Protocol/Set O2 limit|DL-Protocols]] | :::* [[Run DL-Protocol/Set O2 limit|DL-Protocols]] | ||
:::* [[Gnaiger 2008 POS]] | :::* [[Gnaiger 2008 POS]] | ||
:::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]] | :::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]] | ||
:::* [[MiPNet06.03 POS-calibration-SOP|SOP: O<sub>2</sub>-calibration]] | :::* [[MiPNet06.03 POS-calibration-SOP|SOP: O<sub>2</sub>-calibration]] | ||
:::* [https:// | :::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/onOIVpmOEoE]] [https://youtu.be/onOIVpmOEoE O2k-Videosupport: QC1: POS calibration] | ||
=== Part 1.3: O<sub>2</sub> instrumental background === | |||
:::* [[MiPNet14.06 Instrumental O2 background|SOP: O<sub>2</sub> background]] | :::* [[MiPNet14.06 Instrumental O2 background|SOP: O<sub>2</sub> background]] | ||
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:::* [[Mitochondrial preparations]] | :::* [[Mitochondrial preparations]] | ||
:::* [[MiPNet17.03 Shredder vs Fibres|Shredder ''vs'' Fibers]] | :::* [[MiPNet17.03 Shredder vs Fibres|Shredder ''vs'' Fibers]] | ||
:::* [https:// | :::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/EjQGUr5M8v0]] [https://youtu.be/EjQGUr5M8v0 O2k-Videosupport: Shredder tissue homogenate] | ||
:::* [https:// | :::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/iN38rOkiBUQ]] [https://youtu.be/iN38rOkiBUQ O2k-Videosupport: Fiber permeabilization] | ||
:::* [[Cell count and normalization in HRR]] | :::* [[Cell count and normalization in HRR]] | ||
:::* [https:// | :::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/GX_SvnVGXgg]] [https://youtu.be/GX_SvnVGXgg O2k-Videosupport: Addition of biological sample to the respirometer chamber] | ||
'''SUIT protocols, SUITbrowser, DL-Protocols:''' | '''SUIT protocols, SUITbrowser, DL-Protocols:''' | ||
:::* [[MitoPedia: SUIT]] | :::* [[MitoPedia: SUIT]] | ||
:::* [https://suitbrowser.oroboros.at/ Oroboros SUITbrowser] | :::* [https://suitbrowser.oroboros.at/ Oroboros SUITbrowser] | ||
:::* [https:// | :::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/8T33sp9KkJk]] [https://youtu.be/8T33sp9KkJk O2k-Videosupport: How to find a DL-Protocol (DLP)] | ||
:::* [https:// | :::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/Vd66dHx-MyI]] [https://youtu.be/Vd66dHx-MyI O2k-Videosupport: Export DL-Protocol User (*.DLPU)] | ||
=== Part 2.2: Biological experiment and data analysis === | === Part 2.2: Biological experiment and data analysis === | ||
:::* [[MiPNet19.14 SOP Hamilton_microsyringes|SOP: Hamilton microsyringes]] | :::* [[MiPNet19.14 SOP Hamilton_microsyringes|SOP: Hamilton microsyringes]] | ||
:::* [[MiPNet09.12 O2k-Titrations|O2k-Titrations]] | :::* [[MiPNet09.12 O2k-Titrations|O2k-Titrations]] | ||
:::* [https:// | :::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/9j4ZtHmHiss]] [https://youtu.be/9j4ZtHmHiss O2k-Videosupport: How to perform an experiment with a SUIT DL-Protocol (DLP)] | ||
:::* [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|Oxygen flux analysis]] | :::* [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|Oxygen flux analysis]] | ||
:::* [https:// | :::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/HRAGvmaK01I]] [https://youtu.be/HRAGvmaK01I O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4] | ||
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''' O2k-Applications: Simultaneous determination of O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes''' | ''' O2k-Applications: Simultaneous determination of O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes''' | ||
:::* [[Amplex UltraRed]] | :::* [[Amplex UltraRed]] | ||
:::* [https:// | :::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/nlVLXcbBRqs]] [https://youtu.be/nlVLXcbBRqs O2k-Videosupport: Smart Fluo-Sensor assembly] | ||
:::* [https:// | :::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/eUukvgAi1yY]] [https://youtu.be/eUukvgAi1yY O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor] | ||
:::* [[Amplex_UltraRed#Calibration_with_H2O2|Calibration with H<sub>2</sub>O<sub>2</sub>]] | :::* [[Amplex_UltraRed#Calibration_with_H2O2|Calibration with H<sub>2</sub>O<sub>2</sub>]] | ||
:::* [[MiPNet24.10 H2O2 flux analysis|H<sub>2</sub>O<sub>2</sub> flux analysis]] | :::* [[MiPNet24.10 H2O2 flux analysis|H<sub>2</sub>O<sub>2</sub> flux analysis]] | ||
:::* [https:// | :::* [[Image:YouTube Play Button.svg .png|20px|link=https://youtu.be/_Fv0VSA_c14]] [https://youtu.be/_Fv0VSA_c14 O2k-Videosupport: H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4] | ||
Latest revision as of 12:50, 7 November 2023
O2k-Virtual Support self-study material |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
General information
- To benefit optimally from the tutored training sessions of the O2k-Virtual Support, please make yourself familiar with Materials for self-study in advance.
O2k-Basic
Part 1.1: OroboPOS service and O2k instrumental
- O2k-Manual: O2k-FluoRespirometer
- O2k-Manual: POS Service
- Step 1: O2k-Videosupport: OroboPOS from Perspex housing
- Step 2: O2k-Videosupport: Cathode cleaning
- Step 3: O2k-Videosupport: Anode cleaning
- Step 4: O2k-Videosupport: Membrane mounting
- Step 5: O2k-Videosupport: DL7 Start DatLab7
- Step 6: O2k-Videosupport: DL7 Insert OroboPOS
- Step 7: O2k-Videosupport: Insert O2k Chamber
- Step 8: O2k-Videosupport: O2k Chamber volume calibration
Part 1.2: DatLab and O2 calibration
Part 1.3: O2 instrumental background
Part 2.1: Biological samples and experimental design
Pathway control and coupling control of mitochondrial respiration:
Sample types,mitochondrial preparations and sample addition:
SUIT protocols, SUITbrowser, DL-Protocols:
Part 2.2: Biological experiment and data analysis
O2k-Advanced
O2k-Applications: Simultaneous determination of O2 and H2O2 fluxes