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Difference between revisions of "Coupling-control state"

From Bioblast
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|info=[[Gnaiger 2009 Int J Biochem Cell Biol]]
|info=[[Gnaiger 2009 Int J Biochem Cell Biol]]
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{{MitoPedia concepts}}
{{MitoPedia concepts
|mitopedia concept=Respiratory state
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{{MitoPedia methods
{{MitoPedia methods
|mitopedia method=Respirometry
|mitopedia method=Respirometry
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|mitopedia O2k and high-resolution respirometry=SUIT state
|mitopedia O2k and high-resolution respirometry=SUIT state
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{{MitoPedia topics
{{MitoPedia topics}}
|mitopedia topic=Respiratory state
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[[File:EPL-free and excess.jpg|right|300px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014: Fig. 2.4.]]]]
[[File:EPL-free and excess.jpg|right|300px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014: Fig. 2.4.]]]]
'''Coupling states:''' [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ETS capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
'''Coupling states:''' [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ETS capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]


Β  Last edit: [[Gnaiger E]], 2015-10-04
Β  Last edit: [[Gnaiger E]], 2015-10-04

Revision as of 14:59, 8 February 2016


high-resolution terminology - matching measurements at high-resolution


Coupling-control state

Description

Coupling control states are defined in mitochondrial preparations (isolated mitochondria, permeabilized cells, permeabilized tissues, homogenates) as LEAK, OXPHOS, and ETS states of respiration (L, P, E) in any substrate control state which is competent for electron transfer. These coupling states are induced by application of specific inhibitors of the phosphorylation system, titration of ADP and uncouplers. In intact cells, the coupling control states are LEAK, ROUTINE, and ETS states of respiration (L, R, E). Coupling control protocols induce these coupling control states sequentially at a constant substrate control state.


Reference: Gnaiger 2009 Int J Biochem Cell Biol


MitoPedia concepts: Respiratory state 


MitoPedia methods: Respirometry 


MitoPedia O2k and high-resolution respirometry: "SUIT state" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property. SUIT state"SUIT state" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property. 



Coupling states: OXPHOS ROUTINE ETS LEAK - ROX

Last edit: Gnaiger E, 2015-10-04