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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE protocols, terminology, documentation
WG1
- Standard operating procedures and user requirement document: Protocols, terminology, documentation
WG1 Project application
Description
- The quality management system, scope of SOPs to be elaborated and the outline of the data repositories will be defined in a user requirement document.
Respirometric reference protocols
- A ‘library of protocols’ applied in mitochondrial respiratory physiology will be collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of reference protocols will rigorously document (Maelstrom Research program) the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating such bioenergetic tests (see also figure on the right).
Interlaboratory proficiency test
- Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a reference OXPHOS protocol (Fig. 3). The organization of the proficiency test should follow the requirements of the ISO 17043 and 13528 standards. A corresponding MITOEAGLE proficiency training shall be developed and implemented. Feedback from the participants will lead to a final adjustment of the SOPs in the proficiency test, which will be made generally available.
Instrumental platforms
- Comparison of results between and within instrumental platforms: The reference protocols and proficiency test will not define the instrumental platform, but will allow a quantitative comparison of results obtained with specific equipment available in the participating laboratories.
Nomenclature
- Harmonization of nomenclature on mitochondrial respiratory states and control parameters: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MITOEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.
Knowledge management system
- With an increase in data volume and complexity comes along the requirement for standardized report formats and digital accessibility of the data. Since such a database is still missing for mitochondrial physiology, the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. Databases will be established combining biological and clinical data comprising proprietary and publicly available datasets into one integrated solution. Data import and export functionalities will allow data upload and mapping to corresponding objects in the database, in this way generating valuable data and allowing for project-specific as well as cross-project data mining.
- The ability to formulate meta-studies in our approach is an important success factor: The platform can aggregate the project’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental factors associated with disease prevention can be identified and aggregated into the MITOEAGLE Knowledge Management System.
- Querying the heterogeneous data types and result representation are the most important requirements of the knowledge platform besides to data integration and modelling. The application will feature different types of visualization techniques for all integrated data types. The database will be developed using the concept of multi-tier client-server architecture. Individual components are separated corresponding to their functionality. An upstream web server handles user requests, an application server performing business logic, and a back-end database system guaranties persistent data storage. All components are additionally separated by means of network security incorporating firewalls and are hosted in a secure environment. The platform administration ensures privacy regarding proprietary data by user rights policies.
- It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease.
Management
Tasks
- Opening of the database to the research public for data search and moderated entry of new datasets.
- Development of interlaboratory proficiency ring test, of recommendation/certification reports and of a MITOEAGLE proficiency training module.
- Sample distribution, ring test experiments (MITOEAGLE-proficiency training on two different points of time, 6 months apart), data analysis by experimenters and MITOEAGLE-proficiency test manager.
- Reports and publication: Reports with specific results in comparison with an anonymous data summary, and joint publication. Individual reports may be complemented by recommendations, and are summarized in an informal certification report.
- Instrumental platform comparison with reference sample applying comparable measurements.
- Draft, dissemination, collection and final formulation of a manuscript on concepts and terminology of mitochondrial physiology.
- Collect and discuss procedures and experimental protocols for the evaluation of mitochondrial capacities and create a library of protocols: A database comprising reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis is assembled.
- MITOEAGLE data management system (DMS) development: The DMS for further data preprocessing. The DMS will enable management and analysis of instrument-specific and project-specific data.
- MITOEAGLE database (DB) development: DB will facilitate high-level project specific analysis as well as meta-analyses across projects, visualization of project specific data as well as integrative analyses of datasets for various proprietary and/or publicly available datasets.
- Testing of developed software components regarding functionality and usability, modification and implementation. Deployment for routine testing in the labs of our strategic partners.
- Data input: Upload of datasets generated through WG 2-4 in internal lab developments, results of the proficiency ring test, and during application module developments. In addition, the database will be populated with available proprietary and public datasets.
- Develop recommendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation.
- Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
Milestones
- Data sets entered in standardized format and database opened for the public
- Protocols for ring-tests and standard analysis and report scheme defined
- MITOEAGLE proficiency training workshop finished
- Analysis is completed and joint publication submitted
- Publication of paper on concepts and terminology
- Library of protocols online
- Publication of recommended protocols and procedures
- Guidelines for acquisition, evaluation and data documentation available
- MITOEAGLE data management system finished
- MITOEAGLE database ready for use
- Software modifications incorporated following extensive tests
- Publication of MITOEAGLE-KMP
- Recommendations on data acquisition, evaluation and management published
- Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation published
Deliverables
- Guidelines for future research and recommendations for the evaluation of respiratory characteristics in human respirometric samples
- A database on mt fitness evaluated with human cells and tissues
- Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation
- Qualitative and quantitative evaluation of results obtained in ongoing studies by comparison with a reference sample
- An expanded MITOEAGLE database
- A joint publication documenting the results of the MITOEAGLE-PT
- A multi-authored publication towards a unification of concepts and nomenclature in mitochondrial physiology
- A library of protocols for sample preparation and examination by respirometry
- A multi-authored publication with recommendations of comparable standard protocols and procedures.
- Guidelines as to the optimum use of a reference sample for determination of respiration and related parameters of interest.
- The MITOEAGLE data management system.
- A continuously updated MITOEAGLE database for the use as reference data and guideline towards application of optimized protocols, ultimately leading towards standardization.
- A joint publication on the use of the MITOEAGLE database and management system.
- An education programme for the use of MITOEAGLE database and management system.
WG1 Action
- Many analyses lack statistical power within a single study. Statistical combination of different studies is often difficult due to lack of methodological standardization and harmonization.
WG Leader
- Kathrin Renner-Sattler, Mag. Dr. - [email protected]
WG Co-Leader
- Marko Vendelin, Dr. - [email protected]
Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Australia | Nedlands | Perks Kara L |
| Australia | Melbourne, VIC | Muccini Anna Maria |
| Australia | Burwood | Trewin Adam J |
| Australia | Melbourne | Saner Nicholas |
| Australia | Parkville | Hardee Justin P |
| Australia | Syndey | Ton Riccardo |
| Australia | Geelong, Victoria | McKenzie Matthew |
| Australia | Victoria | Winwood-Smith Hugh |
| Australia | Melbourne | Stepto Nigel K |
| Australia | Melbourne | Hewakapuge Sudinna |
| Australia | Newtown, Sydney | Stocker Roland |
| Australia | Melbourne | Lionett S |
| Australia | Queensland | Dong Lan-Feng |
| Australia | Clayton Victoria | Alton Lesley |
| Australia | Victoria | Van Bergen Nicole J |
| Australia | Melbourne | Coughlan Melinda T |
| Australia | Sydney | Hoehn Kyle L |
| Australia | Melbourne | Ferri Alessandra |
| Australia | Melbourne | Cassar Samantha |
| Australia | Melbourne | Genders Amanda J |
| Australia | Sydney | Krycer James R |
| Australia | Queensland | Fisher Joshua J |
| Australia | Queensland | Holland Olivia J |
| Australia | Melbourne | Kuang Jujiao |
| Australia | Queensland | Radenkovic Filip |
| Australia | Clayton | Wolff Jonci Nikolai |
| Australia | Melobourne | Botella Javier |
| Australia | Southport | Vidimce Josif |
| Australia | Nedlands | Filipovska Aleksandra |
| Australia | Melbourne | Whitfield Jamie |
| Austria | Innsbruck | Bergmeister Lisa |
| Austria | Innsbruck | Meszaros Andras T |
| Austria | Innsbruck | Di Marcello Marco |
| Austria | Innsbruck | Antunes Diana |
| Austria | Innsbruck | Goebel G |
| Austria | Innsbruck | Sobotka Ondrej |
| Austria | Kapferer Werner | |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Austria | Innsbruck | Gallee Leon |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Perez Valencia Juan Alberto |
| Austria | Innsbruck | Schwarzer Christoph |
| Austria | Aldrans | Haider Markus |
| Austria | Innsbruck | Klocker Helmut |
| Austria | Innsbruck | Laner Verena |
| Austria | Klosterneuburg | Sazanov Leonid A |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Austria | Innsbruck | Gnaiger Erich |
| Austria | Innsbruck | Schmitt Sabine |
| Austria | Innsbruck | Cecatto Cristiane |
| Austria | Innsbruck | Nindl Veronika |
| Austria | Innsbruck | Passrugger Manuela |
| Austria | Salzburg | Kofler B |
| Austria | Vienna | Kozlov Andrey V |
| Austria | Innsbruck | Keller Markus A |
| Austria | Innsbruck | Burtscher Martin |
| Austria | Innsbruck | Plangger Mario |
| Austria | Innsbruck | Thapa Maheshwor |
| Austria | Innsbruck | Hoppel Florian |
| Austria | Innsbruck | Schartner Melanie |
| Austria | Innsbruck | Doerrier Carolina |
| Belgium | Leuven, Flanders | Baekelandt V |
| Belgium | Namur | Arnould Thierry |
| Belgium | Brussels | Sonveaux P |
| Belgium | Liege | Votion Dominique-Marie |
| Belguim | Sart Tilman (Liège) | Kruse C |
| Brasil | Porto Alegre | Haas Clarissa |
| Brasilia | Limeira | Ropelle Eduardo R |
| Brazil | Sao Paulo | Vercesi Anibal E |
| Brazil | Rio de Janeiro | Galina Antonio |
| Brazil | Rio de Janeiro | Vieyra Adalberto |
| Brazil | Santa Maria - RS | Goncalves Debora Farina |
| Brazil | Santa Maria - Rio Grande do Sul | Soares Felix Alexandre Antunes |
| Brazil | São Paulo | Kowaltowski Alicia J |
| Brazil | Jaboticabal | Oliveira Marcos Tulio |
| Brazil | São Paulo | Crispim Marcell |
| Brazil | Sao Paulo | Silber Ariel Mariano |
| Brazil | Rio de Janeiro | Oliveira Marcus F |
| Canada | Quebec | Joseph Vincent |
| Canada | Edmonton (Alberta) | Lemieux Helene |
| Canada | Edmonton | Zaugg Michael |
| Canada | Saint John | Pulinilkunnil Thomas |
| Canada | Rimouski | Munro Daniel |
| Canada | Charlottetown | Adiele Reginald C |
| Canada | Rimouski | Blier Pierre U |
| Canada | Winnipeg | Chowdhury Subir Roy |
| Canada | Edmonton | Lucchinetti Eliana |
| Canada | Antigonish | Weir M |
| Canada | Hamilton | Tarnopolsky Mark A |
| Canada | Vancouver | Bovard Josh |
| Canada | Guelph | Holloway Graham P |
| Canada | Ottawa | Darveau Charles-A |
| Canada | Montréal | Breton Sophie |
| Canada | Hamilton | Scott Graham R |
| Canada | Ottawa | Harper Mary-Ellen |
| Canada | Montreal, Quebec | Bergdahl Andreas |
| Canada | Edmonton | Han Woo Hyun |
| Canada | Moncton, NB | Pichaud Nicolas |
| Canada | Vancouver, BC | Tausan Daniel |
| Chile | Coyhaique | Jana Prado Fabian |
| China | Tianjin | Zhang Yong |
| China | Pukou, Nanjing, | Gan Zhenji |
| China | Fuzhou, Fujian Province | Chen Qi |
| China | Xi`an | Liu Jiankang |
| Croatia | Zagreb | Saric A |
| Croatia | Zagreb | Curik I |
| Croatia | Zagreb | Rubelj Ivica |
| Croatia | Split | Ljubkovic M |
| Czech Republic | Vestec | Ezrova Zuzana |
| Czech Republic | Praha 2 | Zdrazilova Lucie |
| Czech Republic | Vestec | Rohlena Jakub |
| Czech Republic | Vestec | Davidova Eliska |
| Czech Republic | Hradec Kralove | Stankova Pavla |
| Czech Republic | Hradec Kralove | Cervinkova Zuzana |
| Czech Republic | Pardubice | Rousar Tomas |
| Czech Republic | Prague 10 | Urban Tomas |
| Czech Republic | Prague | Drahota Zdenek |
| Czech Republic | Prague | Krizova J |
| Czech Republic | Prague | Houstek Josef |
| Czech Republic | Hradec Kralove | Endlicher Rene |
| Czech Republic | Plzen -Severni predmesti | Jedlicka Jan |
| Czech Republic | Pardubice | Majtnerova Pavlina |
| Czech Republic | Ceske Budejovice | Zikova Alena |
| Czech Republic | Pardubice | Handl Jiri |
| Czech Republic | Hradec Kralove | Kucera Otto |
| Czech Republic | Praha 2 | Danhelovska Tereza |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Aarhus N | Boetker Hans Erik |
| Denmark | Copenhagen | Nielsen B |
| Denmark | Copenhagen N | Scheibye-Knudsen Morten |
| Denmark | Copenhagen | Larsen Steen |
| Denmark | Copenhagen | Soendergaard SD |
| Denmark | Copenhagen N | Maise Chroeis K |
| Denmark | Copenhagen | Gonzalez-Franquesa Alba |
| Denmark | Copenhagen | Kraunsoee R |
| Denmark | Aarhus N | Jespersen Nichlas Riise |
| Denmark | Roskilde | Dalgaard Louise T |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Copenhagen | Dela Flemming |
| Denmark | Copenhagen N | Mortensen OH |
| Egypt | Cairo | Ali Sameh S |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Estonia | Tallinn | Laasmaa Martin |
| Estonia | Tallinn | Karro N |
| Estonia | Tallinn | Klepinin Aleksandr |
| Estonia | Tallinn | Kaambre Tuuli |
| Estonia | Tallinn | Truu Laura |
| Estonia | Tallinn | Shevchuk Igor |
| Estonia | Tallinn | Tepp Kersti |
| Estonia | Tallinn | Klepinina Lyudmila |
| Estonia | Tallinn | Vendelin Marko |
| Estonia | Tartu | Paju Kalju |
| Estonia | Tallinn | Puurand Marju |
| Finland | Helsinki | Suomalainen Wartiovaara Anu |
| Finland | Helsinki | Jackson Christopher Benjamin |
| Finland | Jyvaeskylae | Kainulainen Heikki |
| Finland | Turku | Stier Antoine |
| France | Paris | Armand Anne-Sophie |
| France | Paris | Bouillaud Frederic |
| France | Angers | Procaccio Vincent |
| France | Nouzilly | Collin-Chenot Anne |
| France | Tours | Dumas Jean-Francois |
| France | Maisons-Alfort Cedex | Prola Alexandre |
| France | Plouzané | Salin Karine |
| France | Lille | Montaigne David |
| France | Martinique | Cano Sanchez Maria Consolacion |
| France | Angers | Spinazzi Marco |
| France | Bordeaux | Amoedo Nivea Dias |
| France | Bordeaux cedex | Mourier A |
| France | Montpellier | Wrutniak-Cabello Chantal |
| France | Gueguen Naig | |
| France | Bordeaux | Sarlak Saharnaz |
| France | Bordeaux cedex | Rossignol Rodrigue |
| France | Martinique | Neviere Remi |
| Germany | Mainz | Methner Axel |
| Germany | Giessen | Sommer Natascha |
| Germany | Bremerhaven | Mark Felix Christopher |
| Germany | Frankfurt | Hamann Andrea |
| Germany | Duesseldorf | Jelenik T |
| Germany | Wickert Anika | |
| Germany | Munich | Zischka Hans |
| Germany | Cologne | Pesta Dominik |
| Germany | Magdeburg | Schoenfeld Peter |
| Germany | Marburg | Vogt Sebastian |
| Germany | Gießen | Grewal Rekha |
| Germany | Gießen | Silaidos Carmina |
| Germany | Cologne | Wiesner Rudolf J |
| Germany | Duesseldorf | Piel Sarah |
| Germany | Doermann Niklas | |
| Germany | Frankfurt am Main | Viel Christian |
| Germany | Cologne | Trifunovic Aleksandra |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Marburg | Ramzan Rabia |
| Germany | Wilhelmshaven | Salmon Pablo |
| Germany | Freiburg | Schuele R |
| Germany | Duesseldorf | Haendeler Judith |
| Germany | Mainz | Maull Felicia |
| Germany | Rostock | Sokolova Inna |
| Germany | Frankfurt | Osiewacz Heinz D |
| Germany | Neuherberg | Einer C |
| Germany | Giessen | Schulz Rainer |
| Germany | Düsseldorf | Goy C |
| Germany | Biberach an der Riß | Nold V |
| Germany | Cologne | Ho Dieu Hien |
| Germany | Frankfurt | Warnsmann Verena |
| Germany | Duesseldorf | Roden Michael |
| Germany | Tuebingen | Kappler Lisa |
| Germany | Ulm | Schaefer PM |
| Germany | Hannover | Das Anibh Martin |
| Germany | Jena | Szibor Marten |
| Germany | Cologne | Maciej Sarah |
| Greece | Thessaloniki | Lazou Antigone |
| Greece | 15771 | Andreadou Ioanna |
| Greece | Athens | Trougakos Ioannis P |
| Greece | Athens | Gumeni Sentiljana |
| Greece | Heraklion | Tavernarakis Nektarios |
| Hungary | Szeged | Ferdinandy Peter |
| Hungary | Budapest | Chinopoulos Christos |
| Hungary | Budapest | Tretter Laszlo |
| Hungary | Budapest | Komlodi Timea |
| Hungary | Szeged | Boros Mihaly |
| Hungary | Debrecen | Verebne Tar K |
| Hungary | Budapest | Horvath G |
| India | Haryana | Chakrabarti Sasanka |
| India | Kollam | Suravajhala Prashanth |
| India | Varanasi | Sonkar Vijay K |
| India | New Delhi | Thakkar Himani |
| India | New Delhi | Vincent Vinnyfred |
| India | Lucknow | Gayen Jiaur |
| India | Hyderabad | Jha Rajan Kumar |
| Iran | Tehran | Shirazi Reza |
| Iran | Tehran | Safaei Zahra |
| Ireland | Dublin | Porter Richard K |
| Ireland | Dublin | O'Gorman Donal |
| Israel | Beer Sheva | Mishmar Dan |
| Israel | Jerusalem | Saada Reisch Ann |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Israel | Zrifin | Sova Marina |
| Italia | Udine | Salvadego Desy |
| Italien | Padova PD | Szabo Ildiko |
| Italy | Rome | Scatena Roberto |
| Italy | Bologna | Paterlini S |
| Italy | Milano | Bottoni P |
| Italy | Padova | Viscomi Carlo |
| Italy | St. Lorenzen | Harrison David K |
| Italy | Bologna | Porcelli AM |
| Italy | Udine | Grassi B |
| Italy | Bolzano | Zanon Alessandra |
| Italy | Padova | Bernardi Paolo |
| Italy | Catania | Messina Angela |
| Italy | Monserrato | Isola Raffaella |
| Italy | Bologna | Gasparre G |
| Italy | Milan | Clementi Emilio |
| Italy | Catania | De Pinto Vito |
| Italy | Catania | Magri Andrea |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Bari | Attimonelli M |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | Bologna | Genova Maria Luisa |
| Italy | Campobasso | Pallotta Maria Luigia |
| Italy | Ancona | Battino Maurizio |
| Japan | Sapporo | Matsumoto J |
| Japan | Itabashi-ku Tokyo | Tanaka Masashi |
| Kitzbühel | Innsbruck | Fischer Michael J |
| Korea | Incheon | Kwak Hyo Bum |
| Korea | Seoul | Pak Youngmi Kim |
| Korea (South) | Seoul | Lee Hong Kyu |
| Latvia | Riga | Zvejniece Liga |
| Latvia | Riga | Stelfa Gundega |
| Latvia | Riga | Liepins Edgars |
| Latvia | Riga | Volska Kristine |
| Latvia | Riga | Inashkina I |
| Latvia | Riga | Jansone Baiba |
| Latvia | Riga | Dambrova Maija |
| Latvia | Riga | Vilks Karlis |
| Latvia | Riga | Svalbe Baiba |
| Luxembourg | Strassen | Devaux Yvan |
| Malaysia | Kuala Lumpur | Hassan Hazirah |
| Malta | Msida | Vella Joanna |
| Mexico | Mexico City | Aparicio Trejo OE |
| Mexico | Tlalpan | Rodriguez-Enriquez Sara |
| México | San Pedro Garza García, Nuevo Leon | Garcia-Rivas Gerardo |
| Netherlands | Maastricht | Grevendonk L |
| Netherlands | GA Wageningen | Grefte Sander |
| New Zealand | Auckland | Hickey Anthony J |
| New Zealand | Auckland | Kaur Sarbjot |
| New Zealand | Wellington | Berridge Michael V |
| New Zealand | Auckland | Ward Marie Louise |
| Norway | Bergen | Hoel Fredrik |
| Norway | Bergen | Roesland Gro Vatne |
| Norway | Bergen | Pettersen Nitschke Ina Katrine |
| Norway | Tromsoe | Boardman Neoma T |
| Norway | Bergen | Tronstad Karl Johan |
| Norway | Bergen | Blindheim Dan Filip |
| Norway | Trondheim | Grill V |
| Norway | Bergen | Berge Rolf K |
| Norway | Trondheim | Lerfall Joergen |
| Norway | Trondheim | Smenes Benedikte Therese |
| Norway | Oslo | Eide L |
| Norway | Bergen | Dyrstad Sissel E |
| Norway | Bergen | Haavik Jan |
| Norway | Trondheim | Rognmo O |
| Poland | Warsaw | Sek Aleksandra |
| Poland | Warsaw | Karkucinska-Wieckowska Agnieszka |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Poland | Warsaw | Wasilewski M |
| Poland | Warsaw | Szewczyk Adam |
| Poland | Warsaw | Bednarczyk Piotr |
| Poland | Warsaw | Wieckowski Mariusz R |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Warszawa | Jaskiewicz Anna |
| Poland | Warsaw | Zablocki Krzysztof |
| Poland | Krakow | Dembinska-Kiec Aldona |
| Poland | Poznan | Jarmuszkiewicz Wieslawa |
| Poland | Gdańsk | Kaczor Jan Jacek |
| Poland | Lodz | Watala Cezary |
| Poland | Warsaw | Dymkowska Dorota |
| Portugal | Coimbra | Duarte Filipe Valente |
| Portugal | Porto | Alves Marco G |
| Portugal | Coimbra | Grilo Luis |
| Portugal | Coimbra | Oliveira Paulo J |
| Portugal | Coimbra | Minuzzi Luciele M |
| Portugal | Coimbra | Rolo Anabela Pinto |
| Portugal | Lisboa | Macedo Maria Paula |
| Portugal | Aveiro | Ferreira Rita Maria P |
| Portugal | Porto | Oliveira Jorge |
| Portugal | Porto | Oliveira Pedro Fontes |
| Portugal | Cantanhede | Rodrigues Ana Sofia |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Porto | Crisostomo Luis |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Porto | Silva Ana Maria |
| Portugal | Cantanhede | Teodoro Joao Soeiro |
| Portugal | Cantanhede | Amorim Ricardo |
| Portugal | Coimbra | Dias Candida |
| Portugal | Porto | Beleza Jorge |
| Portugal | Coimbra | Santos Diana |
| Portugal | Aveiro | Vitorino Rui Miguel Pinheiro |
| Qatar | Doha | Guarch Meritxell Espino |
| Republic of Ghana | Cape Coast | Tei BN |
| Republic of Korea | Busan | Han Jin |
| Republic of Korea | Daejeon | Park SH |
| Republic of Korea | Busan | Kim Hyoung Kyu |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Serbia | Belgrade | Lalic Nebojsa M |
| Republic of Serbia | Novi Sad | Andric Silvana |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Russia | Moscow | Pavlova Nadia |
| Russia | Yoshkar-Ola | Dubinin M |
| Russia | Moscow | Vinogradov Andrey D |
| Serbia | Belgrade | Misirkic Marjanovic Maja |
| Serbia | Belgrade | Mandic M |
| Serbia | Belgrade | Djordjevic M |
| Serbia | Belgrade | Savkovic U |
| Singapore | Singapore | Singh Brijesh Kumar |
| Singapore | Singapore | Hausenloy Derek J |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Slovakia | Bratislava 45 | Ferko Miroslav |
| Slovakia | Košice | Cizmarova Beata |
| Spain | Barcelona | Gil J |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Granada | Quiles JL |
| Spain | Valencia | Casado Pinna Marta |
| Spain | Barcelona | Garcia-Roves Pablo Miguel |
| Spain | Madrid | Hernansanz-Agustin Pablo |
| Spain | Barcelona | De la Torre Lara J |
| Spain | Sevilla | Bustos Matilde |
| Spain | A Coruña | Mayan MD |
| Spain | Salamanca | Almeida Angeles |
| Spain | Madrid | Rial Eduardo |
| Spain | Madrid | Aragones Lopez J |
| Spain | Barcelona | Zorzano Antonio |
| Spain | Las Palmas de Gran Canaria | Calbet Jose AL |
| Spain | Barcelona | Goncalo Teixeira da Silva Rui |
| Spain | Barcelona | Bravo-Sagua Roberto |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Spain | Barcelona | Perales Jose Carles |
| Spain | A Coruña | Blanco FJ |
| Spain | Granada | Hidalgo-Gutierrez A |
| Spain | Madrid | Moran M |
| Sweden | Stockholm | Nedergaard J |
| Sweden | Lund | Ehinger Johannes K |
| Sweden | Stockholm | Larsen Filip J |
| Sweden | Stockholm | Shabalina Irina G |
| Sweden | Gothenburg | Bhuvanachandran NS |
| Sweden | Stockholm | Cardinale Daniele A |
| Switzerland | Lausanne | Lagarrigue Sylviane |
| Switzerland | Basel | Fornaro Mara |
| Switzerland | Basel | Bouitbir Jamal |
| Switzerland | Lausanne | Morato Fornaguera L |
| Switzerland | Lausanne | Donnelly Chris |
| Switzerland | Zurich | Gorr Thomas A |
| Switzerland | Zürich | Zaugg Kathrin |
| Switzerland | Lausanne | Place Nicolas |
| Switzerland | Bern | Nuoffer Jean-Marc |
| Switzerland | Lausanne | Weger M |
| Switzerland | Lausanne | Zanou Nadege |
| Switzerland | Lausanne | Zalachoras I |
| Switzerland | Lausanne | Amati Francesca |
| Switzerland | Lausanne | Canto Alvarez Carles |
| Switzerland | Lausanne | Gebara E |
| Switzerland | Lausanne | Burtscher Johannes |
| Switzerland | Lausanne | Sandi Carmen |
| Switzerland | Bern | Wyss RK |
| Taiwan | Changhua City | Wei Yau-Huei |
| The Netherlands | Maastricht | Nabben Miranda |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| The Netherlands | Groningen | Bakker Barbara M |
| The Netherlands | Delft | McMillan Duncan GG |
| The Netherlands | Maastricht | Schrauwen Patrick |
| UK | Canterbury, Kent | Gourlay Campbell W |
| UK | London | Lane Nick |
| UK | London | Bettinazzi Stefano |
| UK | London | Rodriguez Enrique |
| UK | Cambridge | Brown Guy C |
| USA | Cambridge | Wohlwend Martin |
| USA | Needham | Brown David A |
| USA | Baltimore | Gonzalez-Freire Marta |
| USA | New York | Galkin Alexander |
| USA | Chicago | Dayanidhi Sudarshan |
| USA | San Diego | Patel Hemal H |
| USA | Indianapolis | Brozinick Joseph T |
| USA | Boston | Kristal Bruce S |
| USA | Corvallis | Batterson Philip M |
| USA | Atlanta | Jang Young Charles |
| USA | Miami | Fontanesi F |
| USA | Akron | Cohen Bruce H |
| USA | East Lansing | Zhang Yizhu |
| USA | St. Louis | Abumrad Nada A |
| USA | Davis | Hellgren Kim T |
| USA | Philadelphia | Ganetzky Rebecca |
| USA | Pittsburgh | Jurczak Michael J |
| USA | Winston-Salem | Ahn Bumsoo |
| USA | Torrance | Rossiter Harry B |
| USA | Birmingham | Moellering Douglas R |
| USA | Cleveland | Hoppel Charles L |
| USA | Los Angeles | Kwast Kurt E |
| USA | Fort Collins | Chicco Adam J |
| USA | Stillwater | Davis Michael S |
| USA | Provo | Hancock Chad R |
| USA | San Diego; La Jolla | Molina Anthony JA |
| USA | Iowa City | Wagner Brett A |
| USA | Pittsburgh | Prochownik Edward V |
| USA | Rochester | Nair K Sreekumaran |
| USA | Galveston | Durham William J |
| USA | Aurora | Ford Ellen |
| USA | Ann Arbor | Tyrrell Daniel J |
| USA | Orlando | Sparks Lauren M |
| USA | Milwaukee | Dash Ranjan K |
| USA | Jupiter | Trivigno Catherine |
| USA | Gainesville | Leeuwenburgh Christiaan |
| USA | St. Louis | Pietka Terri A |
| USA | Philadelphia | Jang David H |
| USA | Omaha | Kim Julian KS |
| USA | Davis | Adams Sean H |
| USA | Philadelphia | Towheed Atif |
| USA | San Diego | Cannon Daniel T |
| USA | Norfolk | Lai Nicola |
| USA | Portland | Garlid Keith D |
| USA | Seattle | Marcinek DJ |
| USA | Bethesda | Sharma Pushpa |
| USA | Rochester | Lanza Ian R |
| USA | Corvallis | Newsom Sean A |
| USA | Columbus | Sims Carrie A |
| USA | Stanford | Stary Creed |
| USA | Durham | Li Pingan Andy |
| USA | Kansas City | Swerdlow Russell H |
| USA | Colorado Springs | Jacobs RA |
| USA | Denton | Dzialowski Edward M |
| USA | Orlando | Coen Paul M |
| USA | Orlando | Goodpaster Bret H |
| USA | Fayetteville | Iyer Shilpa |
| USA | Denver | Van Hove Johan |
| USA | Puskarich Michael A | |
| USA | Corvallis | Robinson Matthew M |
| USA | Cleveland | Tandler Bernard |
| USA | El Paso | Bajpeyi Sudip |
| USA | Gainesville | Neyroud D |
| USA | Berkeley | Williams Caroline M |
| USA | Baton Rouge | Irving Brian A |
| USA | Aurora | Sparagna Genevieve C |
| USA | La Jolla | Valentine Joseph Marco |
| USA | Baton Rouge | Hand Steven C |
| USA | Milwaukee | Kandel Sunil Mani |
| USA | Fayetteville | Rao RR |
| USA | Fairbanks | Coker Robert H |
| USA | Philadelphia | Falk Marni J |
| USA | New Haven | Cavalcanti-de-Albuquerque Joao Paulo |
| USA | Bethesda | Fessel Joshua Patrick |
| USA | Little Rock | MacMillan-Crow Lee Ann |
| USA | Nashville | Wasserman David H |
| USA | Orlando | Pino Maria F |
| USA | Little Rock | Borsheim Elisabet |
| USA | Aurora | Moreau Kerrie |
| USA | Greenville | Neufer P Darrell |
| USA | Kansas City | Thyfault John P |
| USA | La Jolla | Schilling Jan M |
| USA | Louisville | Skolik Robert A |
| USA | Wakefield | Varricchio Frederick |
| ... further results | ||
Task groups
- TG leaders: suggestions discussed at the MitoFit Science Camp 2016 Kuehtai AT, and updated according to the MITOEAGLE MC Meeting 2016 Brussels BE.
- TG participants: summary of the feedback received.
TG1.1 Coupling states and coupling control ratios: consensus statements
- TG leaders: Renner-Sattler Kathrin DE / Gnaiger Erich AT / Hoppel Charles L US
- TG participants:
TG1.2 SUIT protocols: nomenclature consistent with concept of database
- TG leader: Doerrier Velasco Carolina A AT, Gnaiger Erich AT
- TG participants: Attimonelli Marcella IT, Krako Nina RS, Vendelin Marko EE
TG1.3 Experimental details in SUIT
- TG leaders: Chicco Adam J US/ Hickey Anthony J NZ/ Doerrier Velasco Carolina A AT
- TG participants: Casado Pinna Marta ES, Krako Nina RS
TG1.4 Instrumental platforms: comparisons
- TG leaders: Elmer Eskil SE (suggested), Molina Anthony JA US
- TG participants: Canto Carles CH (suggested), Genova Maria Luisa IT, Tronstad Karl Johan NO (suggested), Vendelin Marko EE, Zorzano Antonio ES (suggested)
TG1.5 Knowledge management system
- TG leaders: Drinnan Michael UK / Plattner Christina AT
- TG participants: Vendelin Marko EE, Garcia-Roves Pablo ES
TG1.6 Proficiency test
- TG leaders: Gnaiger Erich AT/ Doerrier Velasco Carolina A AT
- TG participants: Genova Maria Luisa IT, Plattner Christina AT
TG1.7 Documentation
- TG leaders: Arnould Thierry BE/ Votion Dominique-Marie BE
- TG participants:
TG1.8 Translation to community in general
- TG leader: Makrecka-Kuka Marina LV (Dissemination Coordinator), Rattan Suresh DK
- TG participants: Krako Nina RS, Irving Brian A US
TG1.9 Dissemination Scientific community
- TG leaders: Makrecka-Kuka Marina LV / Gnaiger Erich AT
- TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Laner Verena AT, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos PT, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE
TG1.10 Training, Short Term Scientific Missions
- TG leader: Labieniec-Watala Magdalena PL, Klaus Susanne DE
- TG participants: Ali Sameh S EG, Cervinkova Zuzana CZ, Chicco Adam J US, Coen Paul M US, Eide Lars NO, Garcia-Roves Pablo M ES, Gasparre Giuseppe IT, Genova Maria Luisa IT, Haendeler Judith DE, Iyer Shilpa US, Krako Nina RS, Mayan Maria D ES, Mishmar Dan IL, Nehlin Jan DK, Palmeira Carlos PT, Paterlini Serena IT, Porcelli Anna Maria IT, Rao Raj US, Schaefer Patrick DE, Tronstad Karl Johan NO, Trougakos Ioannis GR, Volani Chiara AT, Votion Dominique-Marie BE
Comments and discussion
- We are mostly interested in two WG: (1) Since we are working mostly with heart and muscles, we are interested in WG 2 - MITOEAGLE data repository in muscle and other tissues. (2) WG 1 - Standard operating procedures and user requirement document: Protocols, terminology, documentation. Since we have an expertise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabolism. Of course our parts of the WG are also in our interest. - Marina Makrecka-Kuka, Edgars Liepinsh (2016).
- My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on skeletal and cardiac muscle and cultured cardiomyocytes and endothelial cells. I would particularly like to bridge the gap between the group leaders and young investigators, trying to represent the young people who are the active users of the OROBOROS equipment. This will fit in nicely with the goals in WG1. ... Major congratulations on obtaining this beautiful collaborative grant! Very glad to see this network getting the recognition it deserves! Looking forward to being part of this! - Rob C Wuest (2016).
- Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types:
- Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
- Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
- Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - Cezary Watala (2016).
- I have already contacted the portuguese CNC to nominate me for the MC of theaction. Concerning my active participation, taking into account the type of work we do in my lab I could be included in all WGs. As you know we work with mitochondria from liver, muscle and fat, as well as cultured cells from different origins (WG2-4). The elaboration of SOPs (WG1) we can certainly contribute (see the book I edited on Mitochondrial Bioenergetics and in which you contribute a chapter). I believe that everybody can be part of the WG5, but as the coordinators of the action, you and Sandra should decide what is best for the success of the action. - Carlos Palmeira (2016).
- I would propose my participation in the WG1: Standard operating procedures and user requirement document: Protocols, terminology, documentation, considering that the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. I could give my active contribution in the phase of making platform that will aggregate the Action’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases in particular tasks:
- Develop reccomendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalised service for data management, data mining and health-care conforming standards of data interpretation
- Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
- Considering that we are in process of buying the oxygraph, thus broading capacities of my lab, I would be happy to open the door of my new lab for the innovative inter-laboratory ring test experiments and together with my team contribute in that phase of the MITOEAGLE Action. I look forward to the establishment of MitoGlobal EAGLE network where, as stated in the MoU, researchers will collaborate on mapping mitochondrial physiology and medicine. The creation of such a unique well-coordinated network of senior researchers and young investigators will definitely put an added value to existing data and publications, will increase quality and validity of the research, will bring new possibilities of data sharing and data mining and will hopefully bring us to the era of more economical research by saving resources through standardised and harmnonised protocols, where the measuremnts will not be wasted due to lack of comparability. - Nebojsa Lalic (2016).
- Our research interest is oriented to reproductive endocrinology; especially to mechanisms that govern steroidogenesis of testicular Leydig cells in disturbed homeostasis. We use several in vivo models:
- stress -/+ systemic or intratesticular blockade of different stress receptors (alpha1ADR; betaADR; GR)
- hypogonadal-hypogonadism -/+ testosterone-replacement therapy
- androgens anabolics treatment -/+ blockade od AR
- aged rats -/+ Viagra-treatment
- biological clock - Silvana Andric (2016).
- I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation. Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining). - Pablo M Garcia-Roves (2016).
- I will be pleased to contribute to the tasks of WG1 by developing interlaboratory proficiency tests and instrumental platform comparison. Moreover, as a (potential) MC member of MitoEAGLE I can also offer visibility to the Action itself (i.e. WG5: MITOEAGLE dissemination and training, according to mitoglobal.org webpages) by establishing new interactions with the International Coenzyme Q10 Association (ICQA) where I act as a member of the Executive Committee. ICQA organizes biennal conferences on new highlights of Coenzyme Q research. In 2015, we held the conference in Bologna (http://conference2015.wix.com/icqaconference) and we included in the program also some scientific sessions dedicated to mitochondrial bioenergetics and diseases. I will be pleased to enforce cooperation between ICQA and MitoEAGLE. - Maria Luisa Genova (2016).
- We found the scientific content and objectives of the call very interesting and compatible with our wide expertise on oxysterol analysis in food and biological systems. Our group has a large experience on lipid composition and their degradation products (volatile and non-volatile), as well as on sensory analysis. Moreover, we are currently involved in the H2020-GERI-2014-1 project "PLOTINA" ("Promoting gender balance and inclusion in research, innovation and training") with Prof. Gallina Toschi (in cc) as coordinator; the project actually includes gendered case studies where gender and sex variables are considered. You can find further information about our expertises, publications and the PLOTINA project at the following webpages: https://www.unibo.it/sitoweb/maria.rodriguez/, https://www.unibo.it/sitoweb/tullia.gallinatoschi/, http://www.unibo.it/en/research/projects-and-initiatives/research-projects-horizon-2020-1/plotina - Maria Teresa Rodriguez Estrada (2016).
- As you know, our current/developing interests are with "evolution" (species comparisons) and "environment" (hypoxia and cold exposure), but have also experience and interest in assessing effects of "lifestyle" (diet, exercise) and "aging" as well (in rodents). - Adam J Chicco (2016).
- Congratulations on the considerable progress with MITOEAGLE! I am keen to participate in this exciting project and I’m sure I can make a valuable contribution to it's success. I have extensive experience performing respirometry assays in human muscle biopsies, myoblasts/tubes and mouse tissues in the contexts of aging, obesity, exercise and inactivity. I feel that I could contribute to any of the five working groups, but particularly to WG2, WG4, and WG5. I am also happy and willing to serve on the Management Committee if needed. Again, congratulations on organizing such an exciting project. - Paul M Coen (2016).
- I would be interested to be involved with WG1 and WG2. In particular, I am interested in contributing to developing protocols for multicenter quality control among labs, and for permeabilized muscle bundle respirometry. - Harry B Rossiter (2016).
- I think that we could contribute via participation in the evaluation of the protocols, and helping to develop them further. In addition, we have been working on the establishing a database that would allow us to keep protocol details, cell isolation and several other aspects. There is nothing very advanced, but we could contribute to establishing such data collection practice. In addition, we, as opposed to many others, happen to use Strathkelvin respirometer. This alternative hardware would allow us to verify whether the protocols work on them as well. - Marko Vendelin (2016).
Projects in progress
- Interlaboratory proficiency ring test
- Testing of software components
- Concepts and terminology on mitochondrial physiology
- Procedures and experimental protocols for the evaluation of mitochondrial capacities, library of protocols
- Data management system (DMS)
- Database
Next steps
- » MITOEAGLE 2016 Verona IT
- » MITOEAGLE 2017 Barcelona ES
- Best practices for implementation of analytical and process standards.
References
- General requirements for the competence of testing and calibration laboratories » ISO/IEC 17025:2005
