Difference between revisions of "MitoPediaAlert2020 Archive"
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Revision as of 08:11, 8 May 2020
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
May 2020
- May-07 Th
Do you know how to evaluate fatty acid oxidation (FAO, F-pathway) avoiding to overestimate it? Did you know that malate alone might support mt-respiration in mitochondrial preparations, if malate anaplerotic pathways (e.g. mt-malic enzyme) are present?
- Learn more about - »malate anaplerotic pathway control state«
- DatLab 7.4 provides a step-by-step guide through the SUIT-027 to evaluate the malate anaplerotic pathway - »SUIT-027«
- Since F-pathway can be overestimated due to anaplerotic reactions, SUIT-002 was specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways - »SUIT-002«
Previous MitoPedia Alert 2020
>>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
Apr 2020
- Apr-24 Fr
ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.
- Apr-21 Tu
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Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
- Apr-13 Mo
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Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?
DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation
- »SUIT-024«
- Apr-08 We
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The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.
DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration.
- »SUIT-010«
- Apr-02 Th
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Do you want to search the best SUIT protocol for your research questions?
- Use the SUITbrowser: »SUITbrowser«
Mar 2020
- Mar-16 Mo
-
The standardized coupling control protocol to explore the bioenergetics of living cells
DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions.
- »SUIT-003«
- Mar-11 We
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Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2.
- »Blebbistatin«
- Mar-06 Fr
-
Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.
- Mar-03 Tu
-
Rapamycin - more differences or more in common with other mitObesity drugs?
- »Rapamycin«
Feb 2020
- Feb-26 We
-
It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity.
- Feb-24 Mo
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More research is needed on the impact of viral infection on mitochondrial function.
- Feb-19 We
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Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function.
- »Spermidine«
- Feb-18 Tu
-
Why do we fight gas bubbles?
- Dive deep into »Oxygen solubility«
- Feb-12 We
-
What do metformin, melatonin and other bioactive mitObesity compounds have in common?
- »MitObesity drugs«
Jan 2020
- Jan-16 Th
-
Oxygen flux
- which SI units should be used?
-
Did you ever think that units for body mass could be an issue?
- »Body mass«
- Jan-08 We
