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| | » [[MitoPediaAlert2020 Archive]] |
Revision as of 16:17, 5 January 2021
MitoPedia alert
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MitoPedia alert - 2021
Dec 2021
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Why use a short-chain coenzyme Q mimetic for measurement of the Q-redox state using the Q-Module?
The naturally occurring long-isoprenoid chain coenzyme Q (e.g. CoQ10 or CoQ9) is trapped within membrane boundaries. Therefore, a short-chain, membrane-permeable CoQ mimetic, CoQ2 is used as a probe which reacts with the mitochondrial respiratory Complexes at their quinone-binding sites and with the working electrode of the Q-sensor.
- »Coenzmye Q2« and »Q-Module«
- communicated by »Komlodi Timea« and »Schmitt Sabine«
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The central role of the Q-junction in mitochondria
The Q-junction is a point of convergence in the electron transfer system. Electrons flow from different dehydrogenases and respiratory Complexes into the Q-junction and then downstream to Complex III.
- »Q-junction«
- communicated by »Komlodi Timea« and »Schmitt Sabine«
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Coenzyme Q - a mobile redox compound in the electron transfer system
Coenzyme Q is a lipid composed of a benzoquinone ring with an isoprenoid side chain, two methoxy groups and one methyl group being present in all tissues and cells. In humans, CoQ10 occurring in the mitochondrial electron transfer system transfers electrons from dehydrogenases and respiratory complexes downstream to Complex III.
- »Coenzyme Q«
- communicated by »Komlodi Timea« and »Schmitt Sabine«
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Nov 2021
Jul 2021
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TPP+ for potentiometric detection of mt-membrane potential (mtMP) or fluorometry with safranin?
Safranin enables measurement of the relationship between fluorescence intensity and mtMP, and if linear, the absolute mtMP can be calculated. TPP+ enables calibration for measurement of mtMP.
- »TPP+ vs safranin«
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Jun 2021
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Do you know how to select the correct fluorescence sensors and filter set?
Using different fluorescence sensors, LED and photodiode filters, the O2k-Fluo Smart-Module (or O2k-Fluo LED2-Module) enables measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and »Selecting a Filter Set«
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May 2021
Apr 2021
Mar 2021
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Internal normalization of rate – what is the difference between flux control ratio and flux control efficiency?
A flux control ratio is the ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. Flux control efficiency expresses the control of respiration as a fractional change of oxygen flux or flow between protocol steps. Both values vary between theoretical lower and upper limits of 0 and 1.
- »Gnaiger 2020 BEC MitoPathways - Chapter 3«
- » Posted by: »Komlodi Timea« and »Cecatto Cristiane«
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Jan 2021
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Measuring ATP production with Magnesium Green – which sample preparations work?
Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues.
- »MgG« and »Publications MgG«
- communicated by »Cardoso Luiza« and »Komlodi Timea«
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Summary
Stay alert
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» MitoPediaAlert2020 Archive
