Difference between revisions of "O2k-OpenSupportAlert2020 Archive"
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* '''Apr-27 Mo''' | * '''Apr-27 Mo''' | ||
:{| | :{| | ||
| width=" | | width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]] | ||
|'''Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O<sub>2</sub> regime?''' | |'''Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O<sub>2</sub> regime?''' | ||
- To obtain an accurate O<sub>2</sub> background correction, you must select the marks that correspond to your range of experimental O<sub>2</sub> levels. | - To obtain an accurate O<sub>2</sub> background correction, you must select the marks that correspond to your range of experimental O<sub>2</sub> levels. | ||
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* '''Apr-20 Mo''' | * '''Apr-20 Mo''' | ||
:{| | :{| | ||
| width=" | | width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Improve]] | ||
|'''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?''' | |'''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?''' | ||
- Unstable respiratory fluxes in OXPHOS might be due to substrates limitation. | - Unstable respiratory fluxes in OXPHOS might be due to substrates limitation. | ||
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* '''Apr-14 Mo''' | * '''Apr-14 Mo''' | ||
:{| | :{| | ||
| width=" | | width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]] | ||
|'''Why are the oxygen levels different between chambers prior to my experimental run?''' | |'''Why are the oxygen levels different between chambers prior to my experimental run?''' | ||
- After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations. | - After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations. | ||
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* '''Apr-07 Mo''' | * '''Apr-07 Mo''' | ||
:{| | :{| | ||
| width=" | | width="110" align="center" | [[Image:Logo OROBOROS-DatLab.jpg|70px|link=http://wiki.oroboros.at/index.php/DatLab |DatLab]][[File:Blue-info.png|25px|Improve]] | ||
|'''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?''' | |'''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?''' | ||
- If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the '''O2 analysis template DL7.4''' for completion of [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4 |Oxygen flux analysis - DatLab 7.4]]. | - If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the '''O2 analysis template DL7.4''' for completion of [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4 |Oxygen flux analysis - DatLab 7.4]]. | ||
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* '''Apr-02 Mo''' | * '''Apr-02 Mo''' | ||
:{| | :{| | ||
| width=" | | width="110" align="center" | [[Image:SUIT icon.jpg|70px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]][[File:Blue-info.png|25px|Improve]] | ||
|'''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?''' | |'''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?''' | ||
- '''Do you want to search the best [[SUIT]] protocol for your research questions?''' | - '''Do you want to search the best [[SUIT]] protocol for your research questions?''' | ||
Revision as of 10:07, 4 May 2020
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
May 2020
- May-11 Th
Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.
- Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis. - »Steady state«
- May-04 Mo
-
Do you load the correct instrumental background correction accordingly to your range of experimental O2 levels? - See how to perform the instrumental background test. - » O2 background correction in DatLab« and » Instrumental oxygen background«
Apr 2020
- Apr-27 Mo
-
Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O2 regime? - To obtain an accurate O2 background correction, you must select the marks that correspond to your range of experimental O2 levels. - » O2 Background correction - troubleshooting«
- Apr-20 Mo
Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS? - Unstable respiratory fluxes in OXPHOS might be due to substrates limitation. - » Pyruvate - troubleshooting«
- Apr-14 Mo
-
Why are the oxygen levels different between chambers prior to my experimental run? - After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations. - » Oxygen calibration - troubleshooting«
- Apr-07 Mo
Did you know that you can edit the background parameters in the 'O2 analysis template DL7.4? - If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the O2 analysis template DL7.4 for completion of Oxygen flux analysis - DatLab 7.4. - »SUIT: Browse DL-Protocols and templates«
- Apr-02 Mo
Did you know that you can edit the background parameters in the 'O2 analysis template DL7.4? - Do you want to search the best SUIT protocol for your research questions? - Use the SUITbrowser: »SUITbrowser«
Mar 2020
- Mar-16 Mo
The standardized coupling control protocol to explore the bioenergetics of living cells - DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - »SUIT-003«
- Mar-11 We
Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2. - »Blebbistatin«
- Mar-06 Fr
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Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.
- Mar-03 Tu
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Rapamycin - more differences or more in common with other mitObesity drugs? - »Rapamycin«
Feb 2020
- Feb-26 We
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It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity.
- Feb-24 Mo
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More research is needed on the impact of viral infection on mitochondrial function.
- Feb-19 We
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Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. - »Spermidine«
- Feb-18 Tu
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Why do we fight gas bubbles? - Dive deep into »Oxygen solubility«
- Feb-12 We
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What do metformin, melatonin and other bioactive mitObesity compounds have in common? - »MitObesity drugs«
Jan 2020
- Jan-16 Th
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Oxygen flux - which SI units should be used?
-
Did you ever think that units for body mass could be an issue? - »Body mass«
- Jan-08 We
-
Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.
