Difference between revisions of "O2k-OpenSupportAlert2020 Archive"

Revision as of 10:16, 4 May 2020

Currently Answered Questions from the »Oroboros Ecosystem Agenda«

May 2020

May-11 Th

Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.

- Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis. - »Steady state«

May-04 Mo

Do you load the correct instrumental background correction accordingly to your range of experimental O₂ levels?

- See how to perform the instrumental background test. - » O2 background correction in DatLab« and » Instrumental oxygen background«

Apr 2020

Apr-27 Mo

Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O₂ regime?

- To obtain an accurate O₂ background correction, you must select the marks that correspond to your range of experimental O₂ levels. - » O2 Background correction - troubleshooting«

Apr-20 Mo

Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?

- Unstable respiratory fluxes in OXPHOS might be due to substrates limitation. - » Pyruvate - troubleshooting«

Apr-14 Mo

Why are the oxygen levels different between chambers prior to my experimental run?

- After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations. - » Oxygen calibration - troubleshooting«

Apr-07 Mo

Did you know that you can edit the background parameters in the 'O₂ analysis template DL7.4?

- If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the O2 analysis template DL7.4 for completion of Oxygen flux analysis - DatLab 7.4. - »SUIT: Browse DL-Protocols and templates«

Apr-02 Mo

Did you know that you can edit the background parameters in the 'O₂ analysis template DL7.4?

- Do you want to search the best SUIT protocol for your research questions? - Use the SUITbrowser: »SUITbrowser«

Mar 2020

Mar-30 Mo

DatLab 7.4 offers the flexibility to edit a DL-Protocol:

- »Enable DL-Protocol editing«

Mar-25 We

O₂ flux analysis with DatLab 7.4. Use our latest O₂ analysis template DL7.4:

- »O2k-Videosupport: O₂ flux analysis with DatLab 7.4«

Mar-23 Mo

Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O₂ background on the O2k-sV-Module?

- (1) By adjusting the dithionite concentration to 2.5 mM, the instrumental O₂ background can be performed with the "BG Feedback" settings. - »O2k-sV-Module«
- (2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module, the instrumental O₂ background can be performed in the O2k-sV-Module. - »O2k-sV-Module«

Mar-11 We

Do you change the slope smoothing to 20 while running experiments with biological sample?

- While running your experiment, especially during uncoupler titrations, it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - »Slope smoothing«

Mar-04 We

New

A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.

See how you can check that the two blue OroboPOS-Holders are intact. - »O2k-OroboPOS-Holder«

Feb 2020

Feb-26 We

It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity.

- »Noncoupled respiration«

Feb-24 Mo

More research is needed on the impact of viral infection on mitochondrial function.

- »Viruses and mitochondrial medicine«

Feb-19 We

Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function.

- »Spermidine«

Feb-18 Tu

Why do we fight gas bubbles?

- Dive deep into »Oxygen solubility«

Feb-12 We

What do metformin, melatonin and other bioactive mitObesity compounds have in common?

- »MitObesity drugs«

Jan 2020

Jan-16 Th

Oxygen flux

- which SI units should be used?

Did you ever think that units for body mass could be an issue?

- »Body mass«

Jan-08 We

Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.

@@ Line 79: / Line 79: @@
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]
 | '''Do you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background on the [[O2k-sV-Module]]?'''
-- '''(1) By adjusting the [[Dithionite|dithionite]] concentration to  2.5 mM''', the instrumental O<sub>2</sub> background can be performed with the "BG Feedback" settings. - »'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module]]'''«
+- '''(1) By adjusting the [[Dithionite|dithionite]] concentration to  2.5 mM''', the instrumental O<sub>2</sub> background can be performed with the "BG Feedback" settings. - »'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module]]'''« <br>
 - '''(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module''', the instrumental O<sub>2</sub> background can be performed in the O2k-sV-Module. - »'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module]]'''«
 |}
 <br />
+* '''Mar-11 We'''
+:{|
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| '''Do you change the [[Smoothing|slope smoothing]] to 20 while running experiments with biological sample?'''
+- While running your experiment, especially during [[uncoupler titrations]], it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration.
+- »[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]«
+|}
-* '''Mar-11 We'''
-::'''''New''''' [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you change the [[Smoothing|slope smoothing]] to 20 while running experiments with biological sample?'''
-::::::: While running your experiment, especially during [[uncoupler titrations]], it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - »[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]«
 * '''Mar-04 We'''
 [[File:OroboPOS-Holder002.jpg|right|100px]]