O2k-OpenSupportAlert2020 Archive

Currently Answered Questions from the »Oroboros Ecosystem Agenda«

May 2020

May-11 Th

Do you know how to avoid underestimating/overestimating your respiratory fluxes? Measuring steady states with high-resolution respirometry is the key to obtaining correct values.

- Learn more about steady states measured with high-resolution respirometry: performance and mark setting for data analysis. - »Steady state«

May-04 Mo

Do you load the correct instrumental background correction accordingly to your range of experimental O₂ levels?

- See how to perform the instrumental background test. - » O2 background correction in DatLab« and » Instrumental oxygen background«

Apr 2020

Apr-27 Mo

Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O₂ regime?

- To obtain an accurate O₂ background correction, you must select the marks that correspond to your range of experimental O₂ levels. - » O2 Background correction - troubleshooting«

Apr-20 Mo

Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?

- Unstable respiratory fluxes in OXPHOS might be due to substrates limitation. - » Pyruvate - troubleshooting«

Apr-14 Mo

Why are the oxygen levels different between chambers prior to my experimental run?

- After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations. - » Oxygen calibration - troubleshooting«

Apr-07 Mo

Did you know that you can edit the background parameters in the 'O₂ analysis template DL7.4?

- If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the O2 analysis template DL7.4 for completion of Oxygen flux analysis - DatLab 7.4. - »SUIT: Browse DL-Protocols and templates«

Apr-02 Mo

Did you know that you can edit the background parameters in the 'O₂ analysis template DL7.4?

- Do you want to search the best SUIT protocol for your research questions? - Use the SUITbrowser: »SUITbrowser«

Mar 2020

Mar-30 Mo

DatLab 7.4 offers the flexibility to edit a DL-Protocol:

- »Enable DL-Protocol editing«

Mar-25 We

O₂ flux analysis with DatLab 7.4. Use our latest O₂ analysis template DL7.4:

- »O2k-Videosupport: O₂ flux analysis with DatLab 7.4«

Mar-23 Mo

Do you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O₂ background on the O2k-sV-Module?

(1) By adjusting the dithionite concentration to 2.5 mM, the instrumental O₂ background can be performed with the "BG Feedback" settings. - »O2k-sV-Module«
(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module, the instrumental O₂ background can be performed in the O2k-sV-Module. - »O2k-sV-Module«

Mar-11 We

Do you change the slope smoothing to 20 while running experiments with biological sample?

While running your experiment, especially during uncoupler titrations, it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration.
- »Slope smoothing«

Mar-04 We

A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.

See how you can check that the two blue OroboPOS-Holders are intact.
- »O2k-OroboPOS-Holder«

Feb 2020

Feb-26 We

It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity.

- »Noncoupled respiration«

Feb-24 Mo

More research is needed on the impact of viral infection on mitochondrial function.

- »Viruses and mitochondrial medicine«

Feb-19 We

Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function.

- »Spermidine«

Feb-18 Tu

Why do we fight gas bubbles?

- Dive deep into »Oxygen solubility«

Feb-12 We

What do metformin, melatonin and other bioactive mitObesity compounds have in common?

- »MitObesity drugs«

Jan 2020

Jan-16 Th

Oxygen flux

- which SI units should be used?

Did you ever think that units for body mass could be an issue?

- »Body mass«

Jan-08 We

Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.