O2k-Virtual Support self-study material |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
General information
- To benefit optimally from the tutored training sessions of the Virtual O2k-Workshop, please make yourself familiar with Materials for self-study in advance. These materials are either Open Access (OA) or will be sent upon registration (R).
O2k-Basic
Part 1.1: OroboPOS service and O2k instrumental
- O2k-Manual: O2k-FluoRespirometer (OA)
- O2k-Manual: POS Service (OA)
- O2k-Videosupport: OroboPOS (OA)
- O2k-Videosupport: Cathode cleaning (OA)
- O2k-Videosupport: Anode cleaning (OA)
- O2k-Videosupport: Membrane mounting (OA)
- O2k-Videosupport: Start DatLab 7 (OA)
- O2k-Videosupport: Insert OroboPOS (OA)
- O2k-Videosupport: Insert O2k Chamber (OA)
- O2k-Videosupport: O2k Chamber volume calibration (OA)
Part 1.2: DatLab
- MitoPedia: DatLab (OA)
- DL-Protocols (OA)
Part 1.3: O2 calibration and instrumental background
- Gnaiger 2008 POS (OA)
- SOP: O2k-cleaning and ISS (OA)
- SOP: O2-calibration (OA)
- O2k-Videosupport: QC1: POS calibration (R)
- SOP: O2 background (OA)
Part 2.1: Biological samples and experimental design
Pathway control and coupling control of mitochondrial respiration:
Sample types,mitochondrial preparations and sample addition:
- Sample (OA)
- Mitochondrial preparations (OA)
- MiPNet17.03 Shredder vs Fibres (OA)
- O2k-Videosupport: Shredder tissue homogenate (OA)
- O2k-Videosupport: Fiber permeabilization (OA)
- Cell count and normalization in HRR (OA)
- O2k-Videosupport: Addition of biological sample to the respirometer chamber (R)
SUIT protocols, SUITbrowser, DL-Protocols:
Part 2.2: Biological experiment and data analysis
O2k-Advanced
O2k-Applications: Simultaneous determination of O2 and H2O2 fluxes
- Amplex UltraRed (OA)
- O2k-Videosupport: Smart Fluo-Sensor assembly (R)
- O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor (R)
- Calibration with H2O2 (OA)
- H2O2 flux analysis (OA)
- O2k-Videosupport: H2O2 flux analysis with DatLab 7.4 (R)
NextGen-O2k KOL Training
- PB-Module Manual (R)
- O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment (R)
- DL-Protocol: SUIT-030_PB_ce_D069 (R)
- O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment (R)
- DL-Protocol: SUIT-030_PB_ce_D070 (R)