Difference between revisions of "PGM-pathway control state"

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PGM-pathway control state

Description

MitoPathway control state: NADH Electron transfer-pathway state

SUIT protocol: SUIT-001, SUIT-012, SUIT-014

Pyruvate (P) is oxidatively decarboxylated to acetyl-CoA and CO₂, yielding NADH catalyzed by pyruvate dehydrogenase. Malate (M) is oxidized to oxaloacetate by mt-malate dehydrogenase located in the mitochondrial matrix. Condensation of oxaloacate with acetyl-CoA yields citrate (citrate synthase). Glutamate&malate is a substrate combination supporting an N-linked pathway control state, when glutamate is transported into the mt-matrix via the glutamate-aspartate carrier and reacts with oxaloacetate in the transaminase reaction to form aspartate and oxoglutarate. Glutamate as the sole substrate is transported by the electroneutral glutamate^-/OH^- exchanger, and is oxidized in the mitochondrial matrix by glutamate dehydrogenase to α-ketoglutarate ( 2-oxoglutarate), representing the glutamate-anaplerotic pathway control state. 2-oxoglutarate (α-ketoglutarate) is formed from isocitrate (isocitrate dehydrogenase, from oxaloacetate and glutamate by the transaminase, and from glutamate by the glutamate dehydrogenase.

Abbreviation: PGM

Reference: Gnaiger 2014 MitoPathways - Chapter 5.3

MitoPedia concepts: SUIT state

More details

» NADH Electron transfer-pathway state

» Additive effect of convergent electron flow

» Respiratory complexes - more than five

» Convergent electron flow

PGM_L

PGM pathway in the LEAK state can be evaluated in the following SUIT protocols:

PGM_P

PGM pathway in the OXPHOS state can be evaluated in the following SUIT protocols:

SUIT-008

DL-Protocol for permeabilized fibers (pfi): SUIT-008 O2 pfi D014
DL-Protocol for permeabilized cells (pce): SUIT-008 O2 ce-pce D025

PGM_E

PGM pathway in the ET state can be evaluated in the following SUIT protocols:

SUIT-001

DL-Protocol for isolated mitochondria and tissue homogenate (mt):SUIT-001 O2 mt D001
DL-Protocol for permeabilized fibers (pfi):SUIT-001 O2 pfi D002
DL-Protocol for permeabilized cells (pce): SUIT-001 O2 ce-pce D003
DL-Protocol for permeabilized PBMC and PLT: SUIT-001 O2 ce-pce D004

SUIT-012

Discussion

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 '''SUIT protocol:''' [[SUIT-001]], [[SUIT-012]], [[SUIT-014]]
-[[Pyruvate]] (P) is oxidatively decarboxylated to acetyl-CoA and CO<sub>2</sub>, yielding [[NADH]] catalyzed by pyruvate dehydrogenase. [[Malate]] (M) is oxidized to oxaloacetate by mt-malate dehydrogenase located in the mitochondrial matrix. Condensation of oxaloacate with acetyl-CoA yields citrate (citrate synthase). Glutamate&malate is a substrate combination supporting an N-linked pathway control state, when glutamate is transported into the mt-matrix via the [[glutamate-aspartate carrier]] and reacts with [[oxaloacetate]] in the [[transaminase]] reaction to form [[aspartate]] and [[oxoglutarate]]. Glutamate as the sole substrate is transported by the electroneutral glutamate<sup>-</sup>/OH<sup>-</sup> exchanger, and is oxidized in the mitochondrial matrix by [[glutamate dehydrogenase]] to α-ketoglutarate ([[oxoglutarate | 2-oxoglutarate]]), representing the [[glutamate anaplerotic pathway control state]]. 2-oxoglutarate (α-ketoglutarate) is formed from isocitrate (isocitrate dehydrogenase, from oxaloacetate and glutamate by the transaminase, and from glutamate by the glutamate dehydrogenase.
+[[Pyruvate]] (P) is oxidatively decarboxylated to acetyl-CoA and CO<sub>2</sub>, yielding [[NADH]] catalyzed by pyruvate dehydrogenase. [[Malate]] (M) is oxidized to oxaloacetate by mt-malate dehydrogenase located in the mitochondrial matrix. Condensation of oxaloacate with acetyl-CoA yields citrate (citrate synthase). Glutamate&malate is a substrate combination supporting an N-linked pathway control state, when glutamate is transported into the mt-matrix via the [[glutamate-aspartate carrier]] and reacts with [[oxaloacetate]] in the [[transaminase]] reaction to form [[aspartate]] and [[oxoglutarate]]. Glutamate as the sole substrate is transported by the electroneutral glutamate<sup>-</sup>/OH<sup>-</sup> exchanger, and is oxidized in the mitochondrial matrix by [[glutamate dehydrogenase]] to α-ketoglutarate ([[oxoglutarate | 2-oxoglutarate]]), representing the [[glutamate-anaplerotic pathway control state]]. 2-oxoglutarate (α-ketoglutarate) is formed from isocitrate (isocitrate dehydrogenase, from oxaloacetate and glutamate by the transaminase, and from glutamate by the glutamate dehydrogenase.
 |info=[[Gnaiger 2014 MitoPathways |Gnaiger 2014 MitoPathways - Chapter 5.3]]
 }}