Difference between revisions of "SUIT-006 NADH mt D084"
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|info='''A''': protocol for simultaneous determination of O<sub>2</sub> flux and NADH autofluorescence in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-006]]''' | |info='''A''': protocol for simultaneous determination of O<sub>2</sub> flux and NADH autofluorescence in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-006]]''' | ||
|application=NADH | |application=NADH | ||
|SUIT number=D084_1PGM;2D;3U;3anoxia;4Myx | |SUIT number=D084_1PGM;2D;3U;3anoxia;4Myx | ||
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Revision as of 09:57, 30 November 2023
Description
Reference: A: protocol for simultaneous determination of O2 flux and NADH autofluorescence in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- SUIT-006
SUIT number: D084_1PGM;2D;3U;3anoxia;4Myx
O2k-Application: NADH
Communicated by Doerrier C (last update 2022-05-03)
Representative traces
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
mt | REN | mt | ||
1PGM | PGML(n) | N | CI | 1PGM
|
2D | PGMP | N | CI | 1PGM;2D
|
(3Omy) | PGML(Omy) | N | CI | 1PGM;2D;(3Omy)
|
4U | PGME | N | CI | 1PGM;2D;(3Omy);4U
|
5Anox | N | CI | 1PGM;2D;(3Omy);4U;5Anox | |
6Myx | N | CI | 1PGM;2D;(3Omy);4U;5Anox;6Myx
| |
7Reox | ROX | 1PGM;2D;(3Omy);4U;5Anox;6Myx;7Reox |
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- Coupling control
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- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
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Strengths and limitations
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Chemicals and syringes
- Suggested stock concentrations are shown in the specific DL-Protocol.