SUIT-007: Difference between revisions
From Bioblast
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=N(GM) | ||
|description=[[File:|300px]] | |description=[[File:1G;2D;3M;4U;5Ama.png|300px]] | ||
|info='''A:''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-007.pdf|Bioblast pdf]] ยป[http://wiki.oroboros.at/index.php/File:SUIT-007.pdf Versions] | |info='''A:''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-007.pdf|Bioblast pdf]] ยป[http://wiki.oroboros.at/index.php/File:SUIT-007.pdf Versions] | ||
}} | }} | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' N(GM) | ||
::: '''[[SUIT protocol pattern]]:''' | ::: '''[[SUIT protocol pattern]]:''' diametral 1G;2D;2c;3M;4U;5Ama | ||
__TOC__ | __TOC__ | ||
== DLP applications == | == DLP applications == | ||
[[File:ce1;1Dig;2G;3D;4M;5U-.png|300px]] | |||
::::* [[SUIT-007 O2 pce D030]] for permeabilized cells | |||
== References == | == References == | ||
Line 24: | Line 26: | ||
}} | }} | ||
{{Template:}} | {{Template:SUIT-007}} | ||
== Strengths and limitations == | == Strengths and limitations == | ||
:::* Glutamate anaplerotic pathway/glutaminolysis. | |||
:::+ The use of glutamate alone allows to analyse the glutamate anaplerotic pathway control state. | |||
:::+ Short duration of the experiment. | |||
:::+ Glutamate is easier to prepare compared to pyruvate. | |||
:::+ CIV activity can be measured after this protocol. | |||
:::- Analysis of N-OXPHOS capacity (instead of NS-OXPHOS capacity) does not provide the best estimate of maximum mitochondrial respiratory capacity. | |||
:::- This protocol does not address questions regarding the convergence of pathways at the Q-junction. | |||
:::- Careful washing is required after the experiment to avoid carry-over of inhibitors and uncoupler. | |||
Revision as of 16:38, 22 January 2019
Description
Abbreviation: N(GM)
Reference: A: ยปVersions
- SUIT-category: N(GM)
- SUIT protocol pattern: diametral 1G;2D;2c;3M;4U;5Ama
DLP applications
File:Ce1;1Dig;2G;3D;4M;5U-.png
- SUIT-007 O2 pce D030 for permeabilized cells
References
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1G | GL | (N) | CI | 1G
|
2D | GP | (N) | CI | 1G;2D
|
2c | GcP | (N) | CI | 1G;2D;2c
|
3M | GMP | N | CI | 1G;2D;2c;3M
|
4U | GME | N | CI | 1G;2D;2c;3M;4U
|
5Ama | ROX | 1G;2D;2c;3M;4U;5Ama
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- Glutamate anaplerotic pathway/glutaminolysis.
- + The use of glutamate alone allows to analyse the glutamate anaplerotic pathway control state.
- + Short duration of the experiment.
- + Glutamate is easier to prepare compared to pyruvate.
- + CIV activity can be measured after this protocol.
- - Analysis of N-OXPHOS capacity (instead of NS-OXPHOS capacity) does not provide the best estimate of maximum mitochondrial respiratory capacity.
- - This protocol does not address questions regarding the convergence of pathways at the Q-junction.
- - Careful washing is required after the experiment to avoid carry-over of inhibitors and uncoupler.
Compare SUIT protocols
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry