User contributions for Harrison DK

20:3120:31, 25 November 2011 diff hist 0‎ Absorbance ‎No edit summary
20:1520:15, 25 November 2011 diff hist +11‎ White balance ‎No edit summary
20:1320:13, 25 November 2011 diff hist +490‎ N White balance ‎ Created page with "{{MitoPedia |abbr=n.a. |description=In reflectance spectrophotometry and remittance spectrophotometry a white balance is carried out to determine the intensity of the inc..."
20:1020:10, 25 November 2011 diff hist +37‎ Transmittance ‎No edit summary current
20:0920:09, 25 November 2011 diff hist +149‎ N Wavelength averaging ‎ Created page with "{{MitoPedia |abbr=n.a. |description=See smoothing }} {{MitoPedia methods |mitopedia method=Fluorometry, Spectrophotometry }} {{MitoPedia topics}}"
20:0620:06, 25 November 2011 diff hist +101‎ Stray light ‎No edit summary
20:0520:05, 25 November 2011 diff hist +317‎ N Transmittance ‎ Created page with "{{MitoPedia |abbr=T |description=When light enter a sample, '''transmittance''' (''T'') is the fraction of the intensity (''I'') of the light emerging from the sample compared wi..."
20:0120:01, 25 November 2011 diff hist +338‎ N Transmission spectrophotometry ‎ Created page with "{{MitoPedia |abbr=n.a. |description=In the transmission mode, the incident light passes through the sample cuvettes and the emergent light reaches the detector directly. ..."
19:5819:58, 25 November 2011 diff hist +790‎ N Stray light ‎ Created page with "{{MitoPedia |abbr=n.a. |description='''Stray light''' is defined as the detected light of any wavelength that lies outside the bandwidth of the selected wavelength. In the pr..."
19:5219:52, 25 November 2011 diff hist +369‎ N Stability ‎ Created page with "{{MitoPedia |abbr=n.a. |description='''Stability''' determines the accuracy of intensity and absorbance measurements as a function of time. Instability (see drift introdu..."
19:3819:38, 25 November 2011 diff hist +415‎ N Spline ‎ Created page with "{{MitoPedia |abbr=n.a. |description=Some spectrofluorometer or spectrophotometer software offers the possibility of '''spline''' interpolation of the spectral data points..."
19:3519:35, 25 November 2011 diff hist +300‎ N Spectroscopy ‎ Created page with "{{MitoPedia |abbr=n.a. |description='''Spectroscopy''' is a broader term than spectrophotometry in that it is concerned with the investigation and measurement of spectra prod..."
19:3419:34, 25 November 2011 diff hist +392‎ N Spectrophotometry ‎ Created page with "{{MitoPedia |abbr=n.a. |description='''Spectrophotometry''' is the use of a spectrophotometer to measure the transmittance, reflectance or remittance of a material as a funct..."
19:3119:31, 25 November 2011 diff hist +745‎ N Spectrofluorometer ‎ Created page with "{{MitoPedia |abbr=n.a. |description=A '''spectrofluorometer''' makes use of a spectrophotometer to measure and analyse the fluorescent emission spectra from fluorophores...."
19:2719:27, 25 November 2011 diff hist +578‎ N Spectrophotometer ‎ Created page with "{{MitoPedia |abbr=n.a. |description=A '''spectrophotometer''' is an instrument that consists of an entrance slit, a dispersion device (see dispersion devices and a [[detector..."
18:5318:53, 25 November 2011 diff hist +559‎ N Smoothing ‎ Created page with "{{MitoPedia |abbr=n.a. |description=The signal-to-noise ratio can also be improved by spectral ‘’’smoothing’’’. This is achieved by averaging several adjacent dat..."
18:5118:51, 25 November 2011 diff hist +340‎ N Slit width ‎ Created page with "{{MitoPedia |abbr=n.a. |description=The '''slit width''' determines the amount of light entering the spectrofluorometer or spectrophotometer. A larger slit reduces the [[..."
18:4918:49, 25 November 2011 diff hist +382‎ N Signal-to-noise ratio ‎ Created page with "{{MitoPedia |abbr=S/N |description=The '''signal to noise ratio''' is the ratio of the power of the signal to that of the noise. For example, in fluorimetry it would be the r..." current
18:4718:47, 25 November 2011 diff hist +406‎ N Sensitivity ‎ Created page with "{{MitoPedia |abbr=n.a. |description='''Sensitivity''' refers to the response obtained for a given amount of analyte and is often denoted by two factors: the limit of detection an..."
18:4518:45, 25 November 2011 diff hist +378‎ N Selectivity ‎ Created page with "{{MitoPedia |abbr=n.a. |description='''Selectivity''' is the ability of a method to quantify accurately and specifically the analyte or analytes in the presence of other compound..."
18:1818:18, 25 November 2011 diff hist +962‎ N Scattering ‎ Created page with "{{MitoPedia |abbr=n.a. |description=Most biological samples do not consist simply of pigments but also particles (e.g. cells, fibres, mitochondria) which scatter the [[incident l..."
18:1118:11, 25 November 2011 diff hist +516‎ N Safranin ‎ Created page with "{{MitoPedia |abbr=n.a. |description='''Safranin''' is amongst the extrinsic fluorophores used in fluorometry and is one of the most established dyes for measuring mitoch..."
18:0518:05, 25 November 2011 diff hist +509‎ N Resolution ‎ Created page with "{{MitoPedia |abbr=n.a. |description=Spectral resolution is a measure of the ability of an instrument to differentiate between two adjacent wavelengths. Two wavelengths are normal..."
17:5817:58, 25 November 2011 diff hist +405‎ N Remittance spectrophotometry ‎ Created page with "{{MitoPedia |abbr=n.a. |description=In '''remittance spectrophotometry''' incident light enters a scattering medium and is scattered back to the receiving optics (usually..."
17:4917:49, 25 November 2011 diff hist −13‎ Reflectance spectrophotometry ‎No edit summary
17:4817:48, 25 November 2011 diff hist +31‎ Quenching ‎No edit summary
17:4717:47, 25 November 2011 diff hist +333‎ N Reflectance spectrophotometry ‎ Created page with "{{MitoPedia |abbr=n.a. |description=In '''reflectance spectrophotometry''' the light from the sample is reflected back to the detector using mirrors. Before absorbance me..."
17:4417:44, 25 November 2011 diff hist +359‎ N Quenching ‎ Created page with "{{MitoPedia |abbr=n.a. |description='''Quenching''' is the name given to any process that reduces fluorescence intensity. Molecular oxygen is a fluorescence and [[phospho..."
17:4217:42, 25 November 2011 diff hist +332‎ N Precision ‎ Created page with "{{MitoPedia |abbr=n.a. |description='''Precision''' of a method is the degree of agreement among individual test results when the procedure is applied repeatedly to multiple samp..."
17:3917:39, 25 November 2011 diff hist +50‎ Photodiode arrays ‎No edit summary
17:3917:39, 25 November 2011 diff hist +50‎ Photodiodes ‎No edit summary
17:3817:38, 25 November 2011 diff hist +50‎ Phosphorescence ‎No edit summary
17:3717:37, 25 November 2011 diff hist +50‎ Optics ‎No edit summary
17:3617:36, 25 November 2011 diff hist +50‎ Noise ‎No edit summary
17:3417:34, 25 November 2011 diff hist +32‎ N Dispersion Devices ‎ moved Dispersion Devices to Dispersion devices current
17:3417:34, 25 November 2011 diff hist 0‎ m Dispersion devices ‎ moved Dispersion Devices to Dispersion devices
17:2917:29, 25 November 2011 diff hist +417‎ N Photodiode arrays ‎ Created page with "{{MitoPedia |abbr=n.a. |description='''Photodiode arrays''' are two dimensional assemblies of photodiodes. They are frequently used in conjunction with charge coupled device..."
17:2517:25, 25 November 2011 diff hist +486‎ N Photodiodes ‎ Created page with "{{MitoPedia |abbr=n.a. |description='''Photodiodes''' are photodetectors that convert incident light into a current or voltage dependent on their configuration. They have rep..."
17:2217:22, 25 November 2011 diff hist +451‎ N Phosphorescence ‎ Created page with "{{MitoPedia |abbr=n.a. |description='''Phosphorescence''' is a similar phenomenon to fluorescence. However, instead of the electron returning to its original energy state fol..."
17:1817:18, 25 November 2011 diff hist +497‎ N Optics ‎ Created page with "{{MitoPedia |abbr=n.a. |description='''Optics''' are the components that are used to relay and focus light through a spectroflourometer or spectrophotometer. These would ..."
17:1517:15, 25 November 2011 diff hist +569‎ N Noise ‎ Created page with "{{MitoPedia |abbr=n.a. |description=In fluorometry and spectrophotometry, '''noise''' can be attributed to the statistical nature of the photon emission from a [[light so..."
17:0917:09, 25 November 2011 diff hist +10‎ NADH fluorescence ‎No edit summary
17:0517:05, 25 November 2011 diff hist +327‎ N NADH fluorescence ‎ Created page with "{{MitoPedia |abbr=n.a |description=Reduced nicotinamide adenine dinucleotide (NADH) is an intrinsic fluorophore and can be used as a natural indicator of hypoxia. The exc..."

18:5818:58, 18 November 2011 diff hist +515‎ N Multicomponent analysis ‎ Created page with "{{MitoPedia |abbr=Multicomponent analysis |description=Similarly to the least squares method, '''multicomponent analysis''' makes use of all of the data points of the spectru..."
18:4818:48, 18 November 2011 diff hist +658‎ N Microplates ‎ Created page with "{{MitoPedia |abbr=Microplates |description='''Microplate''' readers allow large numbers of sample reactions to be assayed in well format microtitre plates. The most common '''mic..."
18:4118:41, 18 November 2011 diff hist +836‎ N Linearity ‎ Created page with "{{MitoPedia |abbr=Linearity |description='''Linearity''' is the ability of the method to produce test results that are proportional, either directly or by a well-defined mathemat..."
18:3118:31, 18 November 2011 diff hist 0‎ m Lightguides ‎ moved Lighguides to Lightguides
18:3018:30, 18 November 2011 diff hist +546‎ N Lightguides ‎ Created page with "{{MitoPedia |abbr=Lightguides |description='''Lightguides''' consist of optical fibres (either single or in bundles) that can be used to transmit light to a sample from a remote ..."
18:2818:28, 18 November 2011 diff hist +1‎ Filters ‎No edit summary
18:1818:18, 18 November 2011 diff hist 0‎ Balance ‎No edit summary
18:1618:16, 18 November 2011 diff hist 0‎ Balance ‎No edit summary
18:1218:12, 18 November 2011 diff hist +579‎ N Light source ‎ Created page with "{{MitoPedia |abbr=Light source |description=A variety of '''light sources''' are available for fluorimetry and spectrophotometry. These include deuterium, mercury and xen..."
18:0818:08, 18 November 2011 diff hist +503‎ N Least squares method ‎ Created page with "{{MitoPedia |abbr=Least squares method |description=This method makes use of all of the data points of the spectrum in order to quantify a measured spectrum with a reference spec..."
18:0218:02, 18 November 2011 diff hist +359‎ N Intrinsic fluorophores ‎ Created page with "{{MitoPedia |abbr=Intrinsic fluorophores |description=An '''Intrinsic flourophore''' is a naturally occurring fluorophore of which NADH, aromatic amino acids and flavins ..."
17:3017:30, 18 November 2011 diff hist +608‎ N Integration time ‎ Created page with "{{MitoPedia |abbr=Integration time |description='''Integration time''' is the time taken to scan a single full range spectrum using a photodiode array. It is equivalent to t..."
17:2617:26, 18 November 2011 diff hist +223‎ N Incident light ‎ Created page with "{{MitoPedia |abbr=Incident light |description=The term '''incident light''' is used for a beam of light falling upon a surface. }} {{MitoPedia methods |mitopedia method=Fluorimet..."
17:2317:23, 18 November 2011 diff hist +2‎ Fluorophore ‎No edit summary
17:2117:21, 18 November 2011 diff hist +303‎ N Fluorimetry ‎ Created page with "{{MitoPedia |abbr=Fluorimetry |description='''Fluorimetry''' (or fluorometry) is the general term given to the method of measuring the fluorescent emission of a substance followi..."
17:1917:19, 18 November 2011 diff hist +471‎ N Fluorophore ‎ Created page with "{{MitoPedia |abbr=Fluorophore |description=A ''fluorophore'' are fluorescent substances that may occur naturally (intrinsic fluorophores) or that may be added to a sample or ..."
17:1517:15, 18 November 2011 diff hist +158‎ N Fluorescent marker ‎ Created page with "{{MitoPedia |abbr=Fluorescent markers |description=See Extrinsic fluorophores }} {{MitoPedia methods |mitopedia method=Fluorimetry }} {{MitoPedia topics}}"
17:1117:11, 18 November 2011 diff hist +530‎ N Fluorescence ‎ Created page with "{{MitoPedia |abbr=Fluorescence |description='''Fluorescence''' is the name given to light emitted by a substance when it is illuminated (excited) by light at a shorter wavelength..."
17:0917:09, 18 November 2011 diff hist +665‎ N Filters ‎ Created page with "{{MitoPedia |abbr=Filters |description='''Filters''' are materials that have wavelength-dependent transmission characteristics. They are can be used to select the wavelength rang..."
17:0117:01, 18 November 2011 diff hist +509‎ N Extrinsic fluorophores ‎ Created page with "{{MitoPedia |abbr=Extrinsic Fluorophores |description='''Extrinsic fluorophores''' are available for a wide range of parameters including ROS (H2O2, [[Ample..."
16:5116:51, 18 November 2011 diff hist +276‎ N Extinction coefficient ‎ Created page with "{{MitoPedia |abbr=ε |description=The '''extinction coefficient''' (''ε'') of a substance is the absorbance of a 1 µmolar concentration over a 1cm pathlength and is wavele..."
16:4916:49, 18 November 2011 diff hist +173‎ N Extinction ‎ Created page with "{{MitoPedia |abbr=Extinction |description='''Extinction''' is a synonym for absorbance }} {{MitoPedia methods |mitopedia method=Spectrophotometry }} {{MitoPedia topics}}"
16:4716:47, 18 November 2011 diff hist +311‎ N Drift ‎ Created page with "{{MitoPedia |abbr=Drift |description=The most common cause of '''drift''' is variation in the intensity of light sources. The effect of this can be minimised by carrying out ..."
16:4516:45, 18 November 2011 diff hist +403‎ N Dispersion devices ‎ Created page with "{{MitoPedia |abbr=Dispersion devices |description=A '''dispersion device''' diffracts light at different angles according to its wavelength. Traditionally, prisms and [[diffracti..."
16:4316:43, 18 November 2011 diff hist +544‎ N Diffraction gratings ‎ Created page with "{{MitoPedia |abbr=Diffraction Gratings |description='''Diffraction gratings''' are dispersion devices that are made from glass etched with fine grooves, spaced at the same or..."
16:4016:40, 18 November 2011 diff hist +407‎ N Detector ‎ Created page with "{{MitoPedia |abbr=Detector |description=A '''detector''' is a device that converts the light falling upon it into a current or voltage that is proportional to the light intensity..."
16:3816:38, 18 November 2011 diff hist +451‎ N Derivative spectroscopy ‎ Created page with "{{MitoPedia |abbr=Derivative Spectroscopy |description='''Derivative spectroscopy''' can be used to eliminate interfering artefacts or species. A first order derivative will remo..."
16:3016:30, 18 November 2011 diff hist +22‎ N Cuvettes (cells) ‎ moved Cuvettes (cells) to Cuvettes current
16:3016:30, 18 November 2011 diff hist 0‎ m Cuvettes ‎ moved Cuvettes (cells) to Cuvettes
16:2716:27, 18 November 2011 diff hist +40‎ Blank ‎No edit summary
16:2216:22, 18 November 2011 diff hist +578‎ N Cuvettes ‎ Created page with "{{MitoPedia |abbr=Cuvettes |description='''Cuvettes''', or cells, are used in fluorimetry and transmittance spectrophotometry to contain the samples. Traditionally they a..."
16:1816:18, 18 November 2011 diff hist +241‎ N Blank ‎ Created page with "{{MitoPedia |abbr=Blank |description=In transmittance spectrophotometry a '''blank''' cuvette (with no sample in it) is used to carry out the balance. }} {{MitoPedia meth..."
16:1616:16, 18 November 2011 diff hist +551‎ N Beer-Lambert law ‎ Created page with "{{MitoPedia |abbr=B-L law |description=This law states that the transmittance (''T'') of light though a sample is given by: ''T'' = e-''εbc'', where ''ε'' is the..." current
16:1116:11, 18 November 2011 diff hist +327‎ N Bandwidth ‎ Created page with "{{MitoPedia |abbr=Bandwidth |description='''Bandwidth''' is measured in nanometers in terms of the full width half maximum of a peak. This is the portion of the peak that is gre..."
16:0916:09, 18 November 2011 diff hist +50‎ Averaging ‎No edit summary
16:0816:08, 18 November 2011 diff hist +385‎ N Balance ‎ Created page with "{{MitoPedia |abbr=Balance |description=In transmission spectrophotometry a blank cuvette is used to record the incident light intensity (''I''''0'') prior ..."
16:0516:05, 18 November 2011 diff hist +318‎ N Averaging ‎ Created page with "{{MitoPedia |abbr=Averaging |description=In order to improve the signal-to-noise ratio a number of sequential spectra may be averaged over time. The number of spectra to be a..."
16:0216:02, 18 November 2011 diff hist +888‎ N Amplex UltraRed ‎ Created page with "{{MitoPedia |abbr=Amplex red |description='''Amplex red''' is an extrinsic fluorophore that makes use of the fact that ROS generate hydrogen peroxide (H2O..."

14:2514:25, 17 November 2011 diff hist +15‎ Accuracy ‎No edit summary
14:2314:23, 17 November 2011 diff hist −15‎ Accuracy ‎No edit summary
14:2214:22, 17 November 2011 diff hist +14‎ Accuracy ‎No edit summary
14:1914:19, 17 November 2011 diff hist +370‎ N Accuracy ‎ Created page with "{{MitoPedia |description=The ‘’’accuracy’’’ of a method is the degree of agreement between an individual test result generated by the method and the true value. |inf..."
14:0114:01, 17 November 2011 diff hist +72‎ Absorbance ‎No edit summary

13:2913:29, 15 November 2011 diff hist 0‎ Absorbance ‎No edit summary
13:2613:26, 15 November 2011 diff hist +321‎ N Absorbance ‎ Created page with "{{MitoPedia |abbr=''A'' |description=Also known as attenuation, '''absorbance''' (''A'') is a measure of the difference between the incident light intensity (''I''0</sub..."

User contributions for Harrison DK

8 December 2011

7 December 2011

25 November 2011

22 November 2011

18 November 2011

17 November 2011

16 November 2011

15 November 2011

7 November 2011