Difference between revisions of "Talk:Permeabilization of plasma membrane"
From Bioblast
Line 7: | Line 7: | ||
{{MitoPedia O2k and high-resolution respirometry | {{MitoPedia O2k and high-resolution respirometry | ||
|mitopedia MiP and biochemistry =sample preparations}} | |mitopedia MiP and biochemistry =sample preparations}} | ||
__TOC__ | __TOC__ | ||
{{MitoPedia topics | |||
|mitopedia topic=Sample preparation, | |||
[[Mitochondrial preparations]]}} |
Revision as of 11:21, 6 July 2020
- high-resolution terminology - matching measurements at high-resolution
Talk:Permeabilization of plasma membrane
Description
Permeabilization of plasma membrane with optimal digitonin or saponin concentrations yields an effective wash-out of free cytosolic molecules including adenylates, substrates, and cytosolic enzymes. Since the plasma membrane is impermeable to ADP, Cytochrome c, and many other mitochondrial substrates, permeabilization of it gives externally added compounds access to the mitochondria. See also: Permeabilized muscle fibers, Permeabilized cells, Permeabilized tissue.
Reference: Doerrier 2018 Methods Mol Biol
MitoPedia topics:
Sample preparation,
Mitochondrial preparations