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Talk:Permeabilization of plasma membrane
Description
Permeabilization of plasma membrane with optimum digitonin or saponin concentrations yields effective wash-out of free cytosolic molecules including adenylates, substrates, and cytosolic enzymes. Since the plasma membrane is impermeable to ADP, Cytochrome c, and many other mitochondrial substrates, permeabilization of plasma membrane allows the accessibility of externally added compounds to the mitochondria. See also: Permeabilized muscle fibers, Permeabilized cells, Permeabilized tissue.
Reference: Doerrier 2018 Methods Mol Biol
Mitochondrial preparations
