Template talk:Next oroboros ecosystem agenda

SOP: New entry in the Agenda

1. Start a new Current task under your name
2. Enter the alert text to https://wiki.oroboros.at/index.php/Template_talk:Next_oroboros_ecosystem_agenda – check all linked pages, take responsibility as the ‘author’ of text and linked pages.
3. Move the Current task and put it under the name of your targeted 4-eyes colleague (name XY; not the entire science team), add ‘“(from ‘’author name’’)”’ at the end of the ‘Task group’.
4. The Current task will be moved back to you, adding “(from XY)” at the end of the ‘Task group’.
5. If in doubt, ask a more experienced person additionally, with a specific explanation of your question/uncertainty.
6. When the ‘review-process’ is finished, move from https://wiki.oroboros.at/index.php/Template_talk:Next_oroboros_ecosystem_agenda to https://wiki.oroboros.at/index.php/Template:Next_oroboros_ecosystem_agenda

Bioblast alert 2020

Next

Parkinson’s Disease Awareness Month: Dominant mutation in Parkinson’s Disease causes loss of protein function in mitochondria

- from the O2k-Network »FR Angers Gueguen N« and »BE Leuven Spinazzi M«

• Cornelissen T, Spinazzi M, Martin S, Imberechts D, Vangheluwe P, Bird M, De Strooper B, Vandenberghe W (2020) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2. Hum Mol Genet [Epub ahead of print]. »Bioblast link«

Do you want to search the best SUIT protocol for your research questions? Use the SUITbrowser: »SUITbrowser«

Novel diurnal action of of mitochondrial stress-induced GDF15 in systemic metabolism regulation

- from the O2k-Network »DE Nuthetal Klaus S«

• Ost M, Igual Gil C, Coleman V, Keipert S, Efstathiou S, Vidic V, Weyers M, Klaus S (2020) Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress. EMBO Rep [Epub ahead of print]. - »Bioblast link«

An amazing number of new O2k-Publications from China » O2k-Network Labs China «

Let's talk COVID-19 - a new culture of communication in science. - Kay Kupferschmidt in Science

The MitoFit Preprint Archives was created as an Open Access preprint server for mitochondrial physiology and bioenergetics - »MitoFit Preprint Archives«

Zika virus infection leads to mitochondrial dysfunction

- from the O2k-Network »BR Rio de Janeiro Galina A«

• Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK (2020) Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes. Sci Rep 10:1218. - »Bioblast link«

Could exercise protect against the effects of aging on mitochondrial function?

- from the O2k-Network »KR Busan Han J« »KR Incheon Kwak HB«

• No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - »Bioblast link«

On hold

Did you ever think that units for body mass could be an issue? » Body mass «

Why do we fight gas bubbles? - Dive deep into Oxygen solubility

O2k-Publications alert

Next

The mitochondrial gas pedal: Cytosolic calcium controls extramitochondrial pyruvate generation and mitochondrial pyruvate uptake

- from the O2k-Network » FI Helsinki Jacobs HT « » DE Magdeburg Gellerich FN « and»DE Magdeburg Debska-Vielhaber G«

• Szibor Marten, Gizatullina Zemfira, Gainutdinov Timur, Endres Thomas, Debska-Vielhaber Grazyna, Kunz Matthias, Karavasili Niki, Hallmann Kerstin, Schreiber Frank, Bamberger Alexandra, Schwarzer Michael, Doenst Torsten, Heinze Hans-Jochen, Leßmann Volkmar, Vielhaber Stefan, Kunz Wolfram S, Gellerich Frank Norbert (2020) Cytosolic, but not matrix, calcium is essential for adjustment of mitochondrial pyruvate supply. J Biol Chem [Epub ahead of print]. »Bioblast link«

Expression of alternative oxidase prevents reverse electron transfer formation in heart mitochondria isolated from mouse

- from the O2k-Network »FI Helsinki Jacobs HT«  »DE Magdeburg Gellerich FN« »FI Tampere Dufour E« »DE Magdeburg Debska-Vielhaber G« »DE Frankfurt Wittig I« and »UK Brighton Moore AL«

• Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. »Bioblast link«

Mitochondria - a driving force in adipocyte differentiation

- from the O2k-Network »CZ Pilsen Kuncova J«

• Kladnická I, Čedíková M, Kripnerová M, Dvořáková J, Kohoutová M, Tůma Z, Müllerová D, Kuncová J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96. »Bioblast link«

O2k-Open Support alert

Next

• While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.

• Apr-27 Mo

Do you select the appropriate marks for the instrumental background correction when planning to apply them to a protocol with a high O₂ regime?

To obtain an accurate O₂ background correction, you must select the marks that correspond to your range of experimental O₂ levels. - » O2 Background correction - troubleshooting«

• May-04 Mo

Do you load the correct instrumental background correction accordingly to your range of experimental O₂ levels?

See how to perform the instrumental background test. - » O2 background correction in DatLab« and » Instrumental oxygen background«

• May-11 Mo

Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?

Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.-»Switch components approach«

• May-18 Mo

Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?

See how to clean and dry the electrical connections of the OroboPOS-Connector.-»Cleaning the electrical connections«

Removed for correction

Do you perform the leaky chamber test after chamber assembly?

- An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly. - »Leaky chamber test«

» https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly

O2k-Open Support alert circulars

O2k-Open Support alert circular 2020(01): in prep

New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.

See how you can check that the two blue OroboPOS-Holders are intact. - »O2k-OroboPOS-Holder«

New Calibration parameters are transferred to experimental DatLab files

Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - » Oxygen calibration - DatLab«

Do you perform O2k-Chamber volume calibration?

The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - »O2k-Chamber MitoPedia«

Do you use the correct O₂ solubility factor (F_M) in the ‘O2 calibration’ window in DatLab?

The O₂ solubility factor varies according to the medium used and influences the air calibration. - »Oxygen solubility factor«== In preparation ==

Postponed events

 » FAT4BRAIN School Riga LV IOC147«

»APS2020 Chicago US«

San Diego CA US