Difference between revisions of "Template talk:Next oroboros ecosystem agenda"
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::* For 'insufficient uncoupler concentrations' would not be more accurate 'insufficient uncoupler titrations' since we recommend always a titration approach with small volumes to avoid over titration? Maybe by writing 'insufficient uncoupler concentrations' it would lead the user towards using high U concentration per titration instead of using low volumes in each titration? | ::* For 'insufficient uncoupler concentrations' would not be more accurate 'insufficient uncoupler titrations' since we recommend always a titration approach with small volumes to avoid over titration? Maybe by writing 'insufficient uncoupler concentrations' it would lead the user towards using high U concentration per titration instead of using low volumes in each titration? | ||
::* include relevant links (''e.g.,'' for ce and mtprep)? | ::* include relevant links (''e.g.,'' for ce and mtprep)? | ||
::* Not sure if you agree in including the 'E' and 'P' icons within the alert - I thought it would be good just for a quick visualization of the terms. | ::* Not sure if you agree in including the ''E'' and ''P'' icons within the alert - I thought it would be good just for a quick visualization of the terms. | ||
Suggestion 2: | Suggestion 2: |
Revision as of 12:19, 22 June 2020
- Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
{| | logos | title in bold <br> - link |}
e.g.,
Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?
- »Instrumental background« and »TIP2k Filter Papers«
- See the scheme:
Bioblast alert 2020
Prep for circular done here: Talk:Bioblast alert 2020
MitoPedia alert
Did you know that if ET capacity (E) is lower than OXPHOS capacity (P, P>E) in living cells or mitochondrial preparations, then you have encountered an experimental artefact? The possible triggers of this experimental artefact are: - loss of oxidative capacity during the time course of the respirometric assay, Information from: Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »Bioblast link« |
Antunes Diana (talk) 11:13, 22 June 2020 (CEST) Comments and suggestions relevant for checking:
- For 'insufficient uncoupler concentrations' would not be more accurate 'insufficient uncoupler titrations' since we recommend always a titration approach with small volumes to avoid over titration? Maybe by writing 'insufficient uncoupler concentrations' it would lead the user towards using high U concentration per titration instead of using low volumes in each titration?
- include relevant links (e.g., for ce and mtprep)?
- Not sure if you agree in including the E and P icons within the alert - I thought it would be good just for a quick visualization of the terms.
Suggestion 2:
Did you know that if ET capacity is lower than OXPHOS capacity ( P>E, P>E) in living cells or mitochondrial preparations, then you have encountered an experimental artefact? The possible triggers of this experimental artefact are: - loss of oxidative capacity during the time course of the respirometric assay, Information from: Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »Bioblast link« |
Doerrier Carolina (talk) 12:19, 22 June 2020 (CEST)
- For 'insufficient uncoupler concentrations': I think is better 'insufficient uncoupler concentrations' than 'insufficient uncoupler titrations'. Of course, we need to titrate the uncoupler to reach E, but from my point of view 'insufficient uncoupler concentrations' includes: 1) if the uncoupler is not titrated, you have insufficient uncoupler concentration and 2) it can be that we use insufficient uncoupler concentration (for example low uncoupler stock concentration) for a specific sample preparation.
- We can add links to ce and mtprep.
- To include the icons for E and P could be good for visualization.
Then, the entry will be:
Did you know that if ET capacity is lower than OXPHOS capacity (P>E) in living cells or mitochondrial preparations, then you have encountered an experimental artefact? The possible triggers of this experimental artefact are: - loss of oxidative capacity during the time course of the respirometric assay, Information from: Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »Bioblast link« |
Next
- Jul-09 Th (to be confirmed)
Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?
O2k-Brief alert
Next
O2k-Publications alert
Next
- Jun-30 Tu
The antihyperglycemic Empagliflozin protects cardiac mitochondria against high-fat diet-induced dysfunction
- from the O2k-Network »LV Riga Makrecka-Kuka M«
- Makrecka-Kuka M, Korzh S, Videja M, Vilks K, Cirule H, Kuka J, Dambrova M, Liepinsh E (2020) Empagliflozin protects cardiac mitochondrial fatty acid metabolism in a mouse model of diet-induced lipid overload. Cardiovasc Drugs Ther [Epub ahead of print]. »Bioblast link«
O2k-Open Support alert
Next
- While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.
- Jun-29 Mo
or
(NOTE for 4-eyes: QC1: Oxygen sensor test is the O2k-Support page for sensor test, and Noise of 'O2 slope neg.' is the updated version by Erich that belongs to MitoPedia:_DatLab#Calibration. What to do with this pages? Shall the info remain in those locations or shall be linked instead?)
Removed for correction
Do you perform the leaky chamber test after chamber assembly?
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- »Leaky chamber test« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«
O2k-Open Support alert circulars
O2k-Open Support alert circular 2020(01): in prep
New A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
See how you can check that the two blue OroboPOS-Holders are intact.
- »O2k-OroboPOS-Holder«
New Calibration parameters are transferred to experimental DatLab files
Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files.
- » Oxygen calibration - DatLab«
Do you perform O2k-Chamber volume calibration?
The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module).
- »O2k-Chamber MitoPedia«
Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
The O2 solubility factor varies according to the medium used and influences the air calibration.
- »Oxygen solubility factor«
In preparation
For discussion
Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions: - Proven -80 °C solid stability for more than 3 years (3 years 7 months)
- Proven -20 °C solid stability for 1 year
- Proven DMSO stability at room temperature for 1 week
- Proven DMSO stability at -20 °C for 6 months (ongoing)
2021
- 2021-01-06 https://www.jstor.org/stable/1706346?seq=1